Performance of a Nanoribbon Biosensor Sensitized with Aptamers and Antibodies upon Detection of HCVcoreAg<strong> </strong>

Abstract

The performance of the nanoribbon biosensor upon the use of two different types of molecular probes &amp;mdash; the antibodies and the aptamers against HCVcoreAg &amp;mdash; has been tested. The sensor chips employed are based on &amp;ldquo;silicon-on-insulator structures&amp;rdquo;. Two different HCVcoreAg preparations have been tested: recombinant b-galactosidase-conjugated HCVcoreAg (&amp;ldquo;Virogen&amp;rdquo;, USA) and recombinant HCVcoreAg (&amp;ldquo;Vector-Best&amp;rdquo;, Russia). Upon the detection of either type of the antigen preparation, the lowest concentration of the antigen detectable in buffer with pH 5.1 has been found to be approximately equal, amounting to ~10&amp;ndash;14 M. This value has been found to be similar upon the use of either type of molecular probes.