Performance of different protocols on in vitro tuberization in potato (Solanum tuberosum)

Abstract

Five protocols of micro tuberization were used to induce large size microtuber in three recommended potato varieties, namely Cardinal, Diamant, and Heera under complete dark condition. Tuberization was the earliest (11.8 days) in the protocol P 2 (MS + 5 mg/l BAP + 500 mg/l CCC + 8% sucrose), which was closely followed by that in P 1 (12.7 days) (MS + 5 mg/l BAP + 50 mg/l coumarin + 8% sucrose) . Maximum number of microtubers/flask (12.8) was obtained from the protocol P 1 followed by that of P 2 (11.6) that contained growth retardant; but higher average weight of microtuber was obtained in the protocols P 5 (30 days old plantlet + MS media containing 40 meq K + 10 mg/l BA + 9% sucrose), P 4 (MS + 10 mg/l BA + 8% sucrose), and P 3 (MS + 5.0 mg/l BAP + 6% sucrose) which contained BA in absence of growth retardant. The average weight of microtuber was the highest (329.0 mg) in protocol P 5, followed by that in P 4 (280.7 mg), while it was the lowest in protocol P 1 . The variety Diamant produced maximum average weight of microtuber (246.3 mg), while Heera produced minimum (226.1 mg), which was statistically similar to Cardinal (228.7 mg). The highest percentage (52.2) of >300 mg size and lowest percentage (19.3) of <150 mg size microtuber was produced in P 5 protocol in the variety Diamant. On overall consideration, all the varieties performed best with the protocol P 5.

Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria)

“…Protacio and Flores (1992); reported that the free polyamines in explants increased the microtuberization in high and low percentage of sucrose concentrations. Zakaria et al (2014); observed that the highest percentage (52.2%) of >300 mg size and lowest percentage (19.3%) of <150 mg size microtuber was produced in 30 days old plantlet with MS media containing 40 mg.l -1 K + 10 mg.l -1 BA + 9% sucrose in the variety Diamant. Dhital and Lim (2012); reported that 1/2 MS liquid medium containing 8% sucrose and 1.22 gl -1 KNO3, 1.41 gl -1 NH4H2PO4,; 0.23 gl -1 Ca(NO3)24H2O,; and 0.74 gl -1 MgSO4 produced the highest percentage (50%) of medium to large-sized (>500 mg) microtubers.…”

“…A relatively higher number of microtubers observed in treatments with sucrose concentration 3% and 8% while in the control treatment (0%) no microtubers were formed (Wazir et al, 2015). Maximum number of microtubers (12.8) was obtained in MS medium containing 5 mg.l -1 BAP, 50 mg.l -1 coumarin and 8% sucrose that followed by MS medium including 5 mg.l -1 BAP, 500 mg.l -1 CCC and 8% sucrose that contained growth retardant (Zakaria et al, 2014). Gami et al (2013); explained that for microtuber production, high level of sucrose (8%) gave promising results than low level of sucrose (3%).…”

“…It was reported that the increase of microtubers performance by using 10 mg.l -1 of BAP 60 days after the beginning of cultivation increased the average weight of microtubers in comparison with the MS mediums without BAP (Hussey and Stacey, 1984). Hoque (2010); reported that MS medium including 4 mg.l -1 of kinetin with 6% sucrose increased the average weight of microtubers up to 397 mg. Zakaria et al (2014); showed that the higher average weight of microtuber was obtained in the 30 days old plantlet with MS media containing 40 mg.l -1 K + 10 mg.l -1 BAP + 9% sucrose, MS media including 10 mg.l -1 BA and 8% sucrose and MS media with 5.0 mg.l -1 BAP and 6% sucrose which contained BA in absence of growth retardant. Momena et al (2014); reported that maximum average weight was found from MS + 8% Sucrose + 4.00 mg.l -1 Kinetin + 1.00 mg.l -1 BAP (93.47, 97.61, 73.26 and 87.69 mg for Granula, Diamond, Sarpoexona and Lal Pakri1 respectively).…”