Performance of halotolerant bacteria associated with Sahara-inhabiting halophytes Atriplex halimus L. and Lygeum spartum L. ameliorate tomato plant growth and tolerance to saline stress: from selective isolation to genomic analysis of potential determinants

Dif, Guendouz; Belaouni, Hadj Ahmed; Yekkour, Amine; Goudjal, Yacine; Djemouai, Nadjette; Peňázová, Eliška; Čechová, Jana; Berraf-Tebbal, Akila; Eichmeier, Aleš; Zitouni, Abdelghani

doi:10.1007/s11274-021-03203-2

Cited by 6 publications

(7 citation statements)

References 126 publications

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“…These strains are originated from soils with the highest conductivity values. It has been demonstrated in several studies that actinomycetes comprise species that are resistant to high NaCl levels (Dif et al, 2022). Considering this capacity, Sreevidya et al (2016) have demonstrated the capacity of rhizospheric Streptomyces isolates to grow at 8% NaCl and exclusively the isolate SAI-13 to tolerate a concentration of 10% NaCl.…”

Section: In Vivo Bioassay Against S Scabies On Radish Seedlingmentioning

confidence: 99%

“…Our result revealed the siderophores elaboration by three isolates. These findings are of interest, because they have established a positive correlation between plant development and siderophores secretion, and as a result this factor must be included in the selection process of PGP agents (Sreevidya et al, 2016;Ebrahimi-Zarandi et al, 2021;Dif et al, 2022).…”

Section: In Vivo Bioassay Against S Scabies On Radish Seedlingmentioning

confidence: 99%

“…Atriplex halimus L. has been the subject of numerous recent studies around the world, by exploring its related endophytic, rhizospheric, and non-rhizopspheric microbial populations (Tahtamouni et al, 2016;Bona et al, 2021). From the Algerian arid rhizosphere of Atriplex halimus, Dif et al (2022) isolated Gramnegative bacteria able to promote tomato growth through phosphate solubilization, phytohormones and siderophores production.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

et al. 2023

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Biocontrol is considered as an effective alternative to the application of agrochemicals, which are harmful to the environment, human, and animal health. In this study, twenty-six strains of actinomycetes were isolated from rhizospheric arid soil of the halophyte Atriplex halimus L. ῾Guettaf’ in Biskra province, Algeria. The six isolates that have inhibited at least three phytopathogenic fungi among the five tested (Fusarium oxysporum, Alternaria alternata, Fusarium solani, Aspergillus flavus and Botrytis cinerea) were selected, and have been tested in vitro against phytopathogenic bacteria (Pectobacterium carotovorum and Streptomyces scabies). They were also evaluated for their ability to hydrolyze phosphate, elaborate siderophores, produce indole-3-acetic acid (IAA), and to antagonize S. scabies in vivo (on radish seedlings). Based on the physicochemical analyses, soil samples were categorized as alkaline and extremely-saline. The antagonism results revealed varying antifungal potential among the selected isolates (Act11, Act16, Act17, Act18, Act23 and Act24), about 50% were able to inhibit the growth of F. solani and A. flavus, followed by 33.33% of those having antagonized F. oxysporum, while A. alternata was found to be the most sensitive. Only Act18 has antagonized S. scabies in vitro with an inhibition diameter zone of 19 ± 0.41 mm. However, in vivo trials showed that four isolates have counteracted S. scabies. Among them, Act18 and Act24 have significantly and positively affected the root surface (P = 0.0062) and prevented common scab. IAA was detected in all selected isolates with Act24 being the highest producer (77.45 μg mL−1). Additionally, degradation ability revealed that four isolates were able to hydrolyze phosphate while three exhibited the capacity of elaborating siderophores. The six isolates were assigned to Streptomyces genius according to their morphological, physiological and chemotaxonomical traits. Based on this study, Streptomyces sp. Act18 and Streptomyces sp. Act24 that tolerate 7.5% NaCl concentration, prevent common scab and exhibit some plant growth attributes, may be considered as promising biocontrol agents to be applied in arid and saline soils.

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Section: In Vivo Bioassay Against S Scabies On Radish Seedlingmentioning

confidence: 99%

Section: In Vivo Bioassay Against S Scabies On Radish Seedlingmentioning

confidence: 99%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

et al. 2023

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“…SA187 ( Andres-Barrao et al, 2017 ), Bacillus paralicheniformis ES-1 ( Iqbal et al, 2022 ), Brevibacterium sediminis MG-1 ( Lutfullin et al, 2022 ), Bacillus sp. BH32 ( Belaouni et al, 2022 ), Pseudomonas chloritidismutans 6L11 ( Zhou et al, 2022 ), Stenotrophomonas rhizophila IS26 ( Dif et al, 2022 ), Pseudomonas sp. UW4 ( Duan et al, 2013 ), Achromobacter xylosoxidans SQU-1 ( Jana and Yaish, 2021 ), and Stenotrophomonas 169 ( Ulrich et al, 2021 ) are assessed and these genes prevalence are characterized.…”

Section: Introductionmentioning

confidence: 99%

Salinity stress endurance of the plants with the aid of bacterial genes

et al. 2023

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The application of plant growth-promoting bacteria (PGPB) is vital for sustainable agriculture with continuous world population growth and an increase in soil salinity. Salinity is one of the severe abiotic stresses which lessens the productivity of agricultural lands. Plant growth-promoting bacteria are key players in solving this problem and can mitigate salinity stress. The highest of reported halotolerant Plant growth-promoting bacteria belonged to Firmicutes (approximately 50%), Proteobacteria (40%), and Actinobacteria (10%), respectively. The most dominant genera of halotolerant plant growth-promoting bacteria are Bacillus and Pseudomonas. Currently, the identification of new plant growth-promoting bacteria with special beneficial properties is increasingly needed. Moreover, for the effective use of plant growth-promoting bacteria in agriculture, the unknown molecular aspects of their function and interaction with plants must be defined. Omics and meta-omics studies can unreveal these unknown genes and pathways. However, more accurate omics studies need a detailed understanding of so far known molecular mechanisms of plant stress protection by plant growth-promoting bacteria. In this review, the molecular basis of salinity stress mitigation by plant growth-promoting bacteria is presented, the identified genes in the genomes of 20 halotolerant plant growth-promoting bacteria are assessed, and the prevalence of their involved genes is highlighted. The genes related to the synthesis of indole acetic acid (IAA) (70%), siderophores (60%), osmoprotectants (80%), chaperons (40%), 1-aminocyclopropane-1-carboxylate (ACC) deaminase (50%), and antioxidants (50%), phosphate solubilization (60%), and ion homeostasis (80%) were the most common detected genes in the genomes of evaluated halotolerant plant growth-promoting and salinity stress-alleviating bacteria. The most prevalent genes can be applied as candidates for designing molecular markers for screening of new halotolerant plant growth-promoting bacteria.

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“…Since then, HAS strategies employing various halophytes from different saline habitats have been explored to relieve salt stress in salt-sensitive plant species. Some examples of HAS species that confer resistance to salt stress in plants are the halophilic bacteria Stenotrophomonas rhizophila , which inhabits the halophyte Lygeum spartum that is native to the desert environments of Algeria; Bacillus atrophaeus , which is an endophyte of the halophytes Suaeda mollis and S alsola tetrandra that are native to Algeria Salt Lakes; Jejubacter calystegia e, which is found in the halophyte Calystegia soldanella that is found on sandy beaches in South Korea; and many fungus-like microorganisms like Neocamarosporium chichastianum , N. goegapense , and Periconia macropinosa , which has been isolated from the desert halophyte Seidlitzia osmarinus collected at Hoz-e Soltan Salt Lake in Iran ( Jiang et al, 2021 ; Kerbab et al, 2021 ; Moghaddam et al, 2021 ; Dif et al, 2022 ). Some of these HAS species have been successfully employed in PB formulations.…”

Section: Introductionmentioning

confidence: 99%

Habitat-adapted heterologous symbiont Salinispora arenicola promotes growth and alleviates salt stress in tomato crop plants

et al. 2022

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To ensure food security given the current scenario of climate change and the accompanying ecological repercussions, it is essential to search for new technologies and tools for agricultural production. Microorganism-based biostimulants are recognized as sustainable alternatives to traditional agrochemicals to enhance and protect agricultural production. Marine actinobacteria are a well-known source of novel compounds for biotechnological uses. In addition, former studies have suggested that coral symbiont actinobacteria may support co-symbiotic photosynthetic growth and tolerance and increase the probability of corals surviving abiotic stress. We have previously shown that this activity may also hold in terrestrial plants, at least for the actinobacteria Salinispora arenicola during induced heterologous symbiosis with a wild Solanaceae plant Nicotiana attenuata under in vitro conditions. Here, we further explore the heterologous symbiotic association, germination, growth promotion, and stress relieving activity of S. arenicola in tomato plants under agricultural conditions and dig into the possible associated mechanisms. Tomato plants were grown under normal and saline conditions, and germination, bacteria-root system interactions, plant growth, photosynthetic performance, and the expression of salt stress response genes were analyzed. We found an endophytic interaction between S. arenicola and tomato plants, which promotes germination and shoot and root growth under saline or non-saline conditions. Accordingly, photosynthetic and respective photoprotective performance was enhanced in line with the induced increase in photosynthetic pigments. This was further supported by the overexpression of thermal energy dissipation, which fine-tunes energy use efficiency and may prevent the formation of reactive oxygen species in the chloroplast. Furthermore, gene expression analyses suggested that a selective transport channel gene, SlHKT1,2, induced by S. arenicola may assist in relieving salt stress in tomato plants. The fine regulation of photosynthetic and photoprotective responses, as well as the inhibition of the formation of ROS molecules, seems to be related to the induced down-regulation of other salt stress response genes, such as SlDR1A-related genes or SlAOX1b. Our results demonstrate that the marine microbial symbiont S. arenicola establishes heterologous symbiosis in crop plants, promotes growth, and confers saline stress tolerance. Thus, these results open opportunities to further explore the vast array of marine microbes to enhance crop tolerance and food production under the current climate change scenario.

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Performance of halotolerant bacteria associated with Sahara-inhabiting halophytes Atriplex halimus L. and Lygeum spartum L. ameliorate tomato plant growth and tolerance to saline stress: from selective isolation to genomic analysis of potential determinants

Cited by 6 publications

References 126 publications

Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

Salinity stress endurance of the plants with the aid of bacterial genes

Habitat-adapted heterologous symbiont Salinispora arenicola promotes growth and alleviates salt stress in tomato crop plants

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