Performance of LED-Fluorescence microscopy for the detection of acid-fast bacilli from respiratory samples in peripheral laboratories in Argentina

Imaz, María Susana; Allassia, Sonia; Aranibar, Mónica; Gunia, Alba Marisa; Poggi, Susana; Togneri, A; Wolff, Lidia

doi:10.7705/biomedica.v37i2.3276

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…All Sputum samples were examined by the Light emitting diode fluorescence microscope (LED FM) for AFB detection [7]. The AFB appeared as bright yellow against dark back ground materials.…”

Section: Sputum Microscopy For Afbmentioning

confidence: 99%

Bacterial profile and drug susceptibility among adult patients with community acquired lower respiratory tract infection at tertiary hospital, Southern Ethiopia

2021

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Background Lower respiratory tract infection is a global problem accounting over 50 million deaths annually. Here, we determined the bacterial profile and antimicrobial susceptibility pattern of lower respiratory tract infections among adult patients attending at Tertiary Hospital, Southern Ethiopia. Methods A cross sectional study was conducted among adult patients with lower respiratory infection at the medical outpatient department of the Hospital. A sputum sample was collected and processed for bacterial culture and antimicrobial susceptibility test. Semi structured questionnaires were used to collect data. SPSS version 22 software was used for statistical analysis and a p value of < 0.05 was considered as statistically significant. Results Out of 406 sputum samples of participants 136(33.5%) were culture positive for 142 bacterial isolates. Klebsiella pneumoniae 36(25.4%) was the predominant isolate followed by Pseudomonas species 25(17.6%). Gram-negative bacteria were sensitive to cefepime (86.0%) and ciprofloxacin (77.8%) antibiotics while gram-positive (76.5%) to clindamycin. Conclusion Community acquired lower respiratory tract Infection was highly prevalent in the study area and the isolates showed resistant to common antibiotics such as ampicillin, augmentin, ceftazidime and tetracycline. Therefore, culture and susceptibility test is vital for appropriate management of lower respiratory tract infection in the study area.

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“…All Sputum samples were examined by the Light emitting diode fluorescence microscope (LED FM) for AFB detection [7]. The AFB appeared as bright yellow against dark back ground materials.…”

Section: Sputum Microscopy For Afbmentioning

confidence: 99%

Bacterial profile and drug susceptibility among adult patients with community acquired lower respiratory tract infection at tertiary hospital, Southern Ethiopia

2021

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“…All Sputum samples were examined by the Light emitting diode uorescence microscope (LED FM) for AFB detection [7]. The AFB appeared as bright yellow against dark back ground materials.…”

Section: Sputum Microscopy For Afbmentioning

confidence: 99%

Bacterial Profile and Drug Susceptibility among Adult Patients with Community Acquired Lower Respiratory Tract Infection at Tertiary Hospital, Southern Ethiopia

Gebre

Alemayehu

Ormago

2021

Preprint

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Background: Lower respiratory tract infection is a global problem accounting over 50 million deaths annually. Here, we determined the bacterial profile and antimicrobial susceptibility pattern of lower respiratory tract infections among adult patients attending at Tertiary Hospital, Southern Ethiopia. Methods: A cross sectional study was conducted among adult patients with lower respiratory infection at the medical outpatient department of the Hospital. A sputum sample was collected and processed for bacterial culture and antimicrobial susceptibility test. Semi structured questionnaires were used to collect data. SPSS version 22 software was used for statistical analysis and a p value of < 0.05 was considered as statistically significant. Results: Out of 406 sputum samples of participants 136(33.5%) were culture positive for 142 bacterial isolates. Klebsiella pneumoniae 36(25.4%) was the predominant isolate followed by Pseudomonas species 25(17.6%). Gram-negative bacteria were sensitive to cefepime (86.0%) and ciprofloxacin (77.8%) antibiotics while gram-positive (76.5%) to clindamycin. Conclusion: Community acquired lower respiratory tract Infection was highly prevalent in the study area and the isolates showed resistant to common antibiotics such as ampicillin, augmentin, ceftazidime and tetracycline. Therefore, culture and susceptibility test is vital for appropriate management of lower respiratory tract infection in the study area

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“…However, the complexity of the microscope, the requirement for a dark room, the perceived health risk associated with UV light exposure, and the fact that mercury lamps lighting systems are relatively expensive, have a short life span, require stable electrical supply, and new bulbs may be difficult to procure are all downsides of fluorescent microscopes. 7,8 The gold standard for microbiological diagnosis of tuberculosis in underdeveloped countries is culture on Lowenstein Jensen solid medium, which needs roughly 1 bacilli/ml of samples for mycobacterium recovery. Due to the pathogen's delayed growth rate, a definite diagnosis might take up to 4-6 weeks.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Ziehl-Neelsen Stain and Fluorescent Stain Microscopy in the Diagnosis of Pulmonary Tuberculosis

Masali¹,

Takpere²,

Shahapur³

2021

J. Pure Appl. Microbiol.

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Tuberculosis remains a global disease, killing over 2 million people each year. India accounts for two-third of the global total i.e. 26%. A good, fast, and economical test to diagnose a disease is urgently needed so that effective treatment can begin as soon as possible. Various investigations can be done for TB diagnosis and these include clinical suspicion, Chest imaging, acid-fast bacilli staining, MTB culture, serological methods and Assays for amplification of nucleic acids. For developing countries like India due to the enormous number of patients and budget restrictions, the assessment of a quick and low-cost diagnostic procedure is critical. A present prospective study was undertaken to compare Ziehl-Neelsen stain with Fluorescent stain microscopy in detecting of Mycobacterium tuberculosis in TB patient’s sputum samples. Among 274 sputum samples collected from suspected TB cases, 50 (18.2%) were positive by ZN and 116 (42.3%) were positive by fluorescent microscopy. The sensitivity, specificity, Positive predictive value (PPV) & Negative predictive value (NPV) of FM microscopy was 98%, 70%, 42%, and 99% respectively. FM microscopy was superior to ZN microscopy in detecting pulmonary tuberculosis cases as it could detect 24.1% more positive cases than ZN staining. Fluorescence staining provides better sensitivity and specificity and can detect accurately more paucibacillary cases hence is of diagnostic value and can help in the early treatment of pulmonary tuberculosis.

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Performance of LED-Fluorescence microscopy for the detection of acid-fast bacilli from respiratory samples in peripheral laboratories in Argentina

Cited by 4 publications

References 20 publications

Bacterial profile and drug susceptibility among adult patients with community acquired lower respiratory tract infection at tertiary hospital, Southern Ethiopia

Bacterial profile and drug susceptibility among adult patients with community acquired lower respiratory tract infection at tertiary hospital, Southern Ethiopia

Bacterial Profile and Drug Susceptibility among Adult Patients with Community Acquired Lower Respiratory Tract Infection at Tertiary Hospital, Southern Ethiopia

A Comparative Study of Ziehl-Neelsen Stain and Fluorescent Stain Microscopy in the Diagnosis of Pulmonary Tuberculosis

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