Performance of Microflex LT Biotyper and VITEK MS for Routine Identification of Yeasts

Byun, Jung Hyun; Yu, Aiying; Kim, Myung Sook; Lee, Kyungwon

doi:10.3343/alm.2018.38.5.487

Cited by 6 publications

(4 citation statements)

References 10 publications

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“…The clinical strains, apart from the reference strains, were previously identified to the species level by using ITS [ 24 – 26 ] sequencing with the primers ITS1/ITS4(5’-TCCGTAGGTGAACCTGCGG-3’/5’-TCCTCCGCTTATTGATATGC-3’). Additionally, the b-tubulin and calmodulin sequencing techniques were also employed.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Autof ms1000 mass spectrometry for rapid clinical identification of filamentous fungi

Ao,

Li,

Zhang

et al. 2023

BMC Microbiol

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Background Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized microbial identification. However, there is a lack of data on its performance in identifying filamentous fungi. The objective of our study was to evaluate the accuracy of the Autof ms1000 mass spectrometry for identifying filamentous fungi in the clinical microbiology laboratory. Results Among 106 samples tested using the Autof ms1000 system, 101 (95.28%) were identified at the genus or species level, and 81 (76.41%) were accurately identified at the species level. Additionally, we developed a new rapid formic acid extraction method with simple pretreatment for filamentous fungi that saved time and provided accurate results. Conclusions The Autof ms1000 mass spectrometer proved to be a valuable tool for identifying filamentous fungi. However, upgrading the database is recommended for correctly identifying rare strains.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the Autof ms1000 mass spectrometry for rapid clinical identification of filamentous fungi

Ao,

Li,

Zhang

et al. 2023

BMC Microbiol

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“…MALDI Biotyper (Bruker Daltonik Bremen, Germany), and VITEK MS (bioMérieux, Marcy l’Étoile, France) are the two commercially available MALDI-TOF MS systems. Comparison studies regarding the performance of both platforms can be found in the literature [ 119 , 120 , 121 ].…”

Section: Non-conventional Ast Methodsmentioning

confidence: 99%

Rapid Methods for Antimicrobial Resistance Diagnostics

et al. 2021

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Antimicrobial resistance (AMR) is one of the most challenging threats in public health; thus, there is a growing demand for methods and technologies that enable rapid antimicrobial susceptibility testing (AST). The conventional methods and technologies addressing AMR diagnostics and AST employed in clinical microbiology are tedious, with high turnaround times (TAT), and are usually expensive. As a result, empirical antimicrobial therapies are prescribed leading to AMR spread, which in turn causes higher mortality rates and increased healthcare costs. This review describes the developments in current cutting-edge methods and technologies, organized by key enabling research domains, towards fighting the looming AMR menace by employing recent advances in AMR diagnostic tools. First, we summarize the conventional methods addressing AMR detection, surveillance, and AST. Thereafter, we examine more recent non-conventional methods and the advancements in each field, including whole genome sequencing (WGS), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry, Fourier transform infrared (FTIR) spectroscopy, and microfluidics technology. Following, we provide examples of commercially available diagnostic platforms for AST. Finally, perspectives on the implementation of emerging concepts towards developing paradigm-changing technologies and methodologies for AMR diagnostics are discussed.

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“…Additionally, it requires purification, cultivation as well as sample preparation that are not suitable for studying mixed samples ( Wang et al, 2018 ). This being said, MALDI Biotyper and VITEK MS are two commercially available MALDI-TOF MS systems ( Marko Daniel et al, 2012 ; Martiny et al, 2012 ; Byun et al, 2018 ).…”

Section: Relevant Diagnostic Tools For Detecting Lateral Gene Transfe...mentioning

confidence: 99%

Antibiotic resistance in plant growth promoting bacteria: A comprehensive review and future perspectives to mitigate potential gene invasion risks

et al. 2022

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Plant growth-promoting bacteria (PGPB) are endowed with several attributes that can be beneficial for host plants. They opened myriad doors toward green technology approach to reduce the use of chemical inputs, improve soil fertility, and promote plants’ health. However, many of these PGPB harbor antibiotic resistance genes (ARGs). Less attention has been given to multi-resistant bacterial bioinoculants which may transfer their ARGs to native soil microbial communities and other environmental reservoirs including animals, waters, and humans. Therefore, large-scale inoculation of crops by ARGs-harboring bacteria could worsen the evolution and dissemination of antibiotic resistance and aggravate the negative impacts on such ecosystem and ultimately public health. Their introduction into the soil could serve as ARGs invasion which may inter into the food chain. In this review, we underscore the antibiotic resistance of plant-associated bacteria, criticize the lack of consideration for this phenomenon in the screening and application processes, and provide some recommendations as well as a regulation framework relating to the development of bacteria-based biofertilizers to aid maximizing their value and applications in crop improvement while reducing the risks of ARGs invasion.

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Performance of Microflex LT Biotyper and VITEK MS for Routine Identification of Yeasts

Cited by 6 publications

References 10 publications

Evaluation of the Autof ms1000 mass spectrometry for rapid clinical identification of filamentous fungi

Evaluation of the Autof ms1000 mass spectrometry for rapid clinical identification of filamentous fungi

Rapid Methods for Antimicrobial Resistance Diagnostics

Antibiotic resistance in plant growth promoting bacteria: A comprehensive review and future perspectives to mitigate potential gene invasion risks

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