Performing DNA nanotechnology operations on a zebrafish

Yang, Jian; Meng, Zhuojun; Liu, Qing; Olsthoorn, René C. L.; Spaink, Herman P.; Herrmann, Andreas; Kros, Alexander

doi:10.1039/c8sc01771a

Cited by 21 publications

(14 citation statements)

References 40 publications

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“…In 2018, Kros and co‐workers demonstrated the immobilization of oligonucleotides on 2 day old zebrafish and achieved a dramatic improvement of fluorescent labeling efficiency. [ 90 ]…”

Section: Dna Nanostructures On Cell Membranes/synthetic Lipid Bilayermentioning

confidence: 99%

“…The fascinating programmability of DNA origami provides a powerful tool for studying the membrane‐fusion process. In fact, except DNA origami, ssDNA also provides a powerful tool for studying the membrane‐fusion process [ 57,88–90 ] and the exchange of material between liposomes and cells. [ 131,132 ] Our group reported a DNA‐programmed membrane fusion strategy for guiding the efficient intracellular delivery of proteins into live cells in 2018.…”

Section: Application Of Dna Nanostructures In Cell Membrane Engineeringmentioning

confidence: 99%

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Recent Advances of DNA Nanostructure‐Based Cell Membrane Engineering

Feng

Sun

et al. 2021

Adv Healthcare Materials

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Materials that can regulate the composition and structure of the cell membrane to fabricate engineered cells with defined functions are in high demand. Compared with other biomolecules, DNA has unique advantages in cell membrane engineering due to its excellent programmability and biocompatibility. Especially, the near‐atomic scale precision of DNA nanostructures facilitates the investigation of structure–property relations on the cell membrane. In this review, first the state of the art of functional DNA nanostructures is summarized, and then the overview of the use of DNA nanostructures to engineer the cell membrane is presented. Subsequently, applications of DNA nanostructures in modifying cell membrane morphology, controlling ions transport, and synthesizing high precise liposomes are highlighted. Finally, the challenges and outlook on using DNA nanostructures for cell membrane engineering are discussed.

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“…In 2018, Kros and co‐workers demonstrated the immobilization of oligonucleotides on 2 day old zebrafish and achieved a dramatic improvement of fluorescent labeling efficiency. [ 90 ]…”

Section: Dna Nanostructures On Cell Membranes/synthetic Lipid Bilayermentioning

confidence: 99%

Section: Application Of Dna Nanostructures In Cell Membrane Engineeringmentioning

confidence: 99%

Recent Advances of DNA Nanostructure‐Based Cell Membrane Engineering

Feng

Sun

et al. 2021

Adv Healthcare Materials

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“…Interesting research has been carried out employing membrane fusion to directly study and influence biological processes in cells. In terms of drug development, toxicology and nanoparticle characterization, zebrafish have emerged as a simplified in vivo model system and an effective alternative to animals [67] . The Kros group has thoroughly evaluated peptide‐mediated fusion models and has recently implemented them onto zebrafish platforms.…”

Section: Application Of Membrane Fusion Modelsmentioning

confidence: 99%

Membrane Fusion Models for Bioapplications

Mazur

Chandrawati

2021

ChemNanoMat

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Membrane fusion is a highly evolved and significant physiological process involving the mixing of two initially distinct lipid bilayer membranes. It is concerned with communication between and within cells and plays a key role in various processes such as exocytosis, endocytosis, fertilization, viral infection, and carcinogenesis. Due to its significant role, artificial model systems using lipid membranes have been developed over the last few decades which study the membrane fusion mechanism on a molecular level. More recently, this field of research has shifted its attention towards the use of these developed models for a diverse range of biomolecular and biomedical applications. This review will focus on current applications which stem from these models including drug delivery, sensing, protocell proliferation, and others. Our opinions on the future advancements of this field will also be included.

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“…Similar as native protein clusters on cell membranes, DNA nanostructures (not limited to nanopores) might act as artificial gate for intracellular/extracellular transportation, as means for cellular environment regulation and as tool to regulate cellular signaling. From the perspective of synthetic biology, the exciting examples of interfacing DNA amphiphiles with membranes will fuel further activities regarding artificial cell engineering, cell assembly and novel tissue formation . Moreover, DNA amphiphiles might find potential applications in cell‐based therapy.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

DNA Nanotechnology Enters Cell Membranes

Huo

Boersma

et al. 2019

Advanced Science

Self Cite

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DNA is more than a carrier of genetic information: It is a highly versatile structural motif for the assembly of nanostructures, giving rise to a wide range of functionalities. In this regard, the structure programmability is the main advantage of DNA over peptides, proteins, and small molecules. DNA amphiphiles, in which DNA is covalently bound to synthetic hydrophobic moieties, allow interactions of DNA nanostructures with artificial lipid bilayers and cell membranes. These structures have seen rapid growth with great potential for medical applications. In this Review, the current state of the art of the synthesis of DNA amphiphiles and their assembly into nanostructures are first summarized. Next, an overview on the interaction of these DNA amphiphiles with membranes is provided, detailing on the driving forces and the stability of the interaction. Moreover, the interaction with cell surfaces in respect to therapeutics, biological sensing, and cell membrane engineering is highlighted. Finally, the challenges and an outlook on this promising class of DNA hybrid materials are discussed.

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Performing DNA nanotechnology operations on a zebrafish

Abstract: Nanoscale engineering of biological surfaces is becoming an indispensable technique to modify membranes and, thus cellular behaviour.

Cited by 21 publications

References 40 publications

Recent Advances of DNA Nanostructure‐Based Cell Membrane Engineering

Recent Advances of DNA Nanostructure‐Based Cell Membrane Engineering

Membrane Fusion Models for Bioapplications

DNA Nanotechnology Enters Cell Membranes

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