Pericardial fluid and vascular tissue engineering: A preliminary study

Abstract

BACKGROUND: The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE: In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for coating material. In addition to using PFS for surface coating, MED and fetal bovine serum (FBS) were also used for comparison. METHODS: Pericardial fluid cells (PFSc) isolated from PF were cultured on coated PCL scaffolds for 1, 3 and 5 days. Cell viability was determined using 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The MTT assay results showed that the viability of cells on PCL scaffold coated with PFS increased over time (P < 0.005), and cell viability was significantly different between PCL scaffolds coated with PFS and non-coated PCL scaffolds. However, cell viability was significantly higher in the PCL scaffolds coated with PFS than non-coated and coated with FBS, MED and PCL scaffolds. Scanning electron microscopy (SEM) microscopy images and MTT assay indicated that PFSc are attached, proliferated, and spread on PCL scaffolds, especially on PCL scaffolds coated with PFS. CONCLUSIONS: These results suggest that PFS is a biocompatible material for surface modification of PCL scaffolds, which can be used as a suitable material for tissue engineering applications.

A native extracellular matrix material for tissue engineering applications: Characterization of pericardial fluid

“…The harvesting of PF from bovine hearts was described in our previous studies. 42,[46][47][48] Briefly, the thoracic cavity was opened carefully to avoid damaging the pericardium. The PFs were aspirated from the pericardial cavities without removing any organs, by the side of the heart apex with 50-mL sterile syringes, maintained at room temperature, and transported to the laboratory.…”

“…With SEM images and MTT analysis, it was concluded that cell viability and cell adhesion were better in PCL scaffolds coated with PFS on days 1, 3, and 5. 42 The effect of PFS on cell viability and cell retention is that PF contains complex protein structures such as albumin, globulins, macroglobulins, and fibrinogen. 27,42,43 The PCL scaffold is widely used in biomedical research with its biocompatible, mechanically durable, and biodegradable properties; its hydrophobic features negatively affect cell adhesion.…”

“…42 The effect of PFS on cell viability and cell retention is that PF contains complex protein structures such as albumin, globulins, macroglobulins, and fibrinogen. 27,42,43 The PCL scaffold is widely used in biomedical research with its biocompatible, mechanically durable, and biodegradable properties; its hydrophobic features negatively affect cell adhesion. To solve this problem, many natural proteins, such as collagen, gelatin, and fibronectin, are used to increase cell adhesion with surface modification of the PCL scaffold.…”

“…To solve this problem, many natural proteins, such as collagen, gelatin, and fibronectin, are used to increase cell adhesion with surface modification of the PCL scaffold. [41][42][43][44][45] Taken together with all this characteristic of the PF, we hypothesized that PF can be used for tissue engineering applications such as using for scaffold production materials, coating material, using for ideal ECM mimetic, and increasing biocompatibility of biomaterials.…”

Investigation of the Interaction of Adipose-Derived Mesenchymal Stem Cells with ε-Polycaprolactone and Egg White Scaffolds

“…Biodegradable, low-molecular-weight (10 kDa), and high-molecular-weight (85 kDa) ε-PCLs, approved by the Food and Drug Administration (FDA), have been synthesized from ε-caprolactone monomers under tin octoate catalyst following earlier studies [26,27]. The synthesized ε-PCL scaffolds were prepared by mixing low-molecular-weight (80%) and high-molecular-weight polymers (20%).…”