Perineuronal nets in the auditory system

Sonntag, Mandy; Blosa, Maren; Schmidt, Sophie; Rübsamen, Rudolf; Morawski, Markus

doi:10.1016/j.heares.2014.12.012

Cited by 43 publications

(62 citation statements)

References 138 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the expression of PNN and its association with specific cell types have been well characterized in rodent visual and somatosensory cortex (Pizzorusso et al, 2002; McRae et al, 2007; Liu et al, 2013; Takesian and Hensch, 2013) and subcortical auditory areas (Beebe et al, 2016), the expression patterns in A1 are only beginning to be described (reviewed in Sonntag et al, 2015). The relationship between PV and PNN expression in A1 has not been previously characterized.…”

Section: Discussionmentioning

confidence: 99%

Age-Related Deterioration of Perineuronal Nets in the Primary Auditory Cortex of Mice

Brewton

Kokash

Jimenez

et al. 2016

Front. Aging Neurosci.

View full text Add to dashboard Cite

Age-related changes in inhibitory neurotransmission in sensory cortex may underlie deficits in sensory function. Perineuronal nets (PNNs) are extracellular matrix components that ensheath some inhibitory neurons, particularly parvalbumin positive (PV+) interneurons. PNNs may protect PV+ cells from oxidative stress and help establish their rapid spiking properties. Although PNN expression has been well characterized during development, possible changes in aging sensory cortex have not been investigated. Here we tested the hypothesis that PNN+, PV+ and PV/PNN co-localized cell densities decline with age in the primary auditory cortex (A1). This hypothesis was tested using immunohistochemistry in two strains of mice (C57BL/6 and CBA/CaJ) with different susceptibility to age-related hearing loss and at three different age ranges (1–3, 6–8 and 14–24 months old). We report that PNN+ and PV/PNN co-localized cell densities decline significantly with age in A1 in both mouse strains. In the PNN+ cells that remain in the old group, the intensity of PNN staining is reduced in the C57 strain, but not the CBA strain. PV+ cell density also declines only in the C57, but not the CBA, mouse suggesting a potential exacerbation of age-effects by hearing loss in the PV/PNN system. Taken together, these data suggest that PNN deterioration may be a key component of altered inhibition in the aging sensory cortex, that may lead to altered synaptic function, susceptibility to oxidative stress and processing deficits.

show abstract

Section: Discussionmentioning

confidence: 99%

Age-Related Deterioration of Perineuronal Nets in the Primary Auditory Cortex of Mice

Brewton

Kokash

Jimenez

et al. 2016

Front. Aging Neurosci.

View full text Add to dashboard Cite

show abstract

“…8B and D, left panel). Furthermore, Gα i3 expression itself was encircled by the perineuronal net marker brevican [64] (Fig. 8B and D, right panel).…”

Section: Function Of Gα I3 In the Central Auditory Pathway Correlatesmentioning

confidence: 96%

Gαi Proteins are Indispensable for Hearing

Beer‐Hammer

Lee

Mauriac

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: From invertebrates to mammals, Gα_i proteins act together with their common binding partner Gpsm2 to govern cell polarization and planar organization in virtually any polarized cell. Recently, we demonstrated that Gα_i3-deficiency in pre-hearing murine cochleae pointed to a role of Gα_i3 for asymmetric migration of the kinocilium as well as the orientation and shape of the stereociliary (“hair”) bundle, a requirement for the progression of mature hearing. We found that the lack of Gα_i3 impairs stereociliary elongation and hair bundle shape in high-frequency cochlear regions, linked to elevated hearing thresholds for high-frequency sound. How these morphological defects translate into hearing phenotypes is not clear. Methods: Here, we studied global and conditional Gnai3 and Gnai2 mouse mutants deficient for either one or both Gα_i proteins. Comparative analyses of global versus Foxg1-driven conditional mutants that mainly delete in the inner ear and telencephalon in combination with functional tests were applied to dissect essential and redundant functions of different Gα_i isoforms and to assign specific defects to outer or inner hair cells, the auditory nerve, satellite cells or central auditory neurons. Results: Here we report that lack of Gα_i3 but not of the ubiquitously expressed Gα_i2 elevates hearing threshold, accompanied by impaired hair bundle elongation and shape in high-frequency cochlear regions. During the crucial reprogramming of the immature inner hair cell (IHC) synapse into a functional sensory synapse of the mature IHC deficiency for Gα_i2 or Gα_i3 had no impact. In contrast, double-deficiency for Gα_i2 and Gα_i3 isoforms results in abnormalities along the entire tonotopic axis including profound deafness associated with stereocilia defects. In these mice, postnatal IHC synapse maturation is also impaired. In addition, the analysis of conditional versus global Gα_i3-deficient mice revealed that the amplitude of ABR wave IV was disproportionally elevated in comparison to ABR wave I indicating that Gα_i3 is selectively involved in generation of neural gain during auditory processing. Conclusion: We propose a so far unrecognized complexity of isoform-specific and overlapping Gα_i protein functions particular during final differentiation processes.

show abstract

“…PNNs are prevalent in the adult rodent auditory system, including the auditory cortex (Sonntag et al, 2015). Given that these structures play a role in the inhibition of plasticity and closure of ocular dominance critical periods in the visual cortex, it is possible that they play a role in cementing or stabilizing the newly formed auditory memory traces in the auditory cortex, resulting in fear learning and consolidation.…”

Section: Introductionmentioning

confidence: 99%

Perineuronal Nets in the Adult Sensory Cortex Are Necessary for Fear Learning

Banerjee

Gutzeit

Baman

et al. 2017

Neuron

130

106

View full text Add to dashboard Cite

Summary Lattice-like structures known as perineuronal nets (PNNs) are key components of the extracellular matrix (ECM). Once fully crystallized by adulthood, they are largely stable throughout life. Contrary to previous reports that PNNs inhibit processes involving plasticity, here we report that the dynamic regulation of PNN expression in the adult auditory cortex is vital for fear learning and consolidation in response to pure tones. Specifically, after first confirming the necessity of auditory cortical activity for fear learning and consolidation, we observed that mRNA levels of key proteoglycan components of PNNs were enhanced 4 hours after fear conditioning but were no longer different from the control groups 24 hours later. A similar pattern of regulation was observed in numbers of cells surrounded by PNNs and area occupied by them in the auditory cortex. Finally, the removal of auditory cortex PNNs resulted in a deficit in fear learning and consolidation.

show abstract

Perineuronal nets in the auditory system

Cited by 43 publications

References 138 publications

Age-Related Deterioration of Perineuronal Nets in the Primary Auditory Cortex of Mice

Age-Related Deterioration of Perineuronal Nets in the Primary Auditory Cortex of Mice

Gαi Proteins are Indispensable for Hearing

Perineuronal Nets in the Adult Sensory Cortex Are Necessary for Fear Learning

Contact Info

Product

Resources

About