Periodic Acid Oxidation

Jackson, Elizabeth B.

doi:10.1002/0471264180.or002.08

Cited by 14 publications

(7 citation statements)

References 132 publications

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“…Therefore, GMS staining is highly identifiable, revealing the fungal cell wall as brown-black, while other histological structures are barely visible. In contrast, due to the "weak" oxidation of periodic acid, PAS staining can only oxidize hydroxyl groups to aldehyde groups (Jackson, 2011). It results in a large number of positive tissues and cells (Layton and Bancroft, 2019), which explains the defects of the principle of PAS staining of fungi; the present findings are consistent with this staining principle.…”

Section: Discussionsupporting

confidence: 80%

Grocott Methenamine Silver Staining Is the Optimal Approach to Histological Diagnosis of Pulmonary Cryptococcosis

Wang

Lai

et al. 2022

Front. Microbiol.

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BackgroundHistological staining methods for Cryptococcus identification vary in accuracy. This study aimed to investigate the clinical value of Grocott methenamine silver (GMS), periodic acid-Schiff (PAS), and Alcian blue (AB) staining in the diagnosis of pulmonary cryptococcosis (PC).MethodsFrom April 2004 to June 2021, the clinical and pathological data of 152 patients with PC were collected from the Department of Pathology, Sun Yat-sen University Cancer Center. The sensitivity and identifiability of GMS, PAS, and AB staining for histological diagnosis were systematically evaluated using statistical methods combined with the microscopic characteristics of PC cases.ResultsStatistical analysis showed that the detection rates of GMS, PAS, and AB staining were 100.0% (152/152), 94.7% (144/152), and 81.6% (124/152), respectively. McNemar’s test showed that the sensitivity of GMS was significantly higher than those of PAS (P = 0.008) and AB stains (P < 0.001). Both PAS and AB stains had obvious non-specific staining, which interfered with the detection of Cryptococcus, and increased diagnostic difficulties. In contrast, in GMS staining, Cryptococcus spores were prominent with a clean background and were clearly observed at low or medium power magnification, with the identifiability significantly better than those of PAS or AB staining.ConclusionGMS staining had sensitivity up to 100%, and identifiability that was better than those of PAS and AB staining. GMS is the best method for histological diagnosis of PC.

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Section: Discussionsupporting

confidence: 80%

Grocott Methenamine Silver Staining Is the Optimal Approach to Histological Diagnosis of Pulmonary Cryptococcosis

Wang

Lai

et al. 2022

Front. Microbiol.

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“…Although it would be useful to know the exact amino acids in β that are crosslinked, which we have achieved with other PhK crosslinkers, that would not be trivial with periodate, given that it likely modifies numerous residues not involved in crosslinking. Periodate has been reported to oxidize the side chains of Tyr, Met, Trp, Cys, Asp, Asn, Arg, and His but the only crosslink that has been characterized is a 3,3′‐dityrosine that it formed in ovotransferrin . A variety of crosslinking reagents has been successfully used to study subunit interactions in PhK, but only two of these have been zero‐length, forming α–α, α–β, and γ–δ dimers .…”

Section: Discussionmentioning

confidence: 99%

A model for activation of the hexadecameric phosphorylase kinase complex deduced from zero‐length oxidative crosslinking

2015

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Phosphorylase kinase (PhK) is a hexadecameric (abcd) 4 enzyme complex that upon activation by phosphorylation stimulates glycogenolysis. Due to its large size (1.3 MDa), elucidating the structural changes associated with the activation of PhK has been challenging, although phosphoactivation has been linked with an increased tendency of the enzyme's regulatory b-subunits to self-associate. Here we report the effect of a peptide mimetic of the phosphoryltable N-termini of b on the selective, zero-length, oxidative crosslinking of these regulatory subunits to form b-b dimers in the nonactivated PhK complex. This peptide stimulated b-b dimer formation when not phosphorylated, but was considerably less effective in its phosphorylated form. Because this peptide mimetic of b competes with its counterpart region in the nonactivated enzyme complex in binding to the catalytic c-subunit, we were able to formulate a structural model for the phosphoactivation of PhK. In this model, the nonactivated state of PhK is maintained by the interaction between the nonphosphorylated N-termini of b and the regulatory C-terminal domains of the c-subunits; phosphorylation of b weakens this interaction, leading to activation of the c-subunits.

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“…A solution containing 1 mg/ml of the polysaccharide was oxidized with 0.02M NalO^ in the dark at 5 C. At,intervals up to 220 hours, 1 ml aliquots were removed for measurement of periodate consumption by the arsenite method of Jackson (1944), using 0.005M for the titrations.…”

Section: Periodate Oxidation and Borohydride Reductionmentioning

confidence: 99%

The stalk polysaccharide of the diatom Gomphonema olivaceum

Huntsman

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Periodic Acid Oxidation

Cited by 14 publications

References 132 publications

Grocott Methenamine Silver Staining Is the Optimal Approach to Histological Diagnosis of Pulmonary Cryptococcosis

Grocott Methenamine Silver Staining Is the Optimal Approach to Histological Diagnosis of Pulmonary Cryptococcosis

A model for activation of the hexadecameric phosphorylase kinase complex deduced from zero‐length oxidative crosslinking

The stalk polysaccharide of the diatom Gomphonema olivaceum

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