Periodontal tissue engineering: current strategies and the role of platelet rich hemoderivatives

Babo, Pedro S.; Reis, Rui L.; Gomes, Manuela E.

doi:10.1039/c7tb00010c

Cited by 23 publications

(14 citation statements)

References 115 publications

(180 reference statements)

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“…Another important drawback associated with PL--based gels is the lack of control over dose and spatio--temporal delivery of specific platelet--derived biomolecules due to the low stability of the gel fibrillar matrix 9,10 . In an in vivo scenario, fibrin(ogen) is known for its ability to bind GFs through its heparin--binding domain and uses this affinity based mechanism to control the sequestering and subsequent release of signaling biomolecules…”

Section: Resultsmentioning

confidence: 99%

Human-based fibrillar nanocomposite hydrogels as bioinstructive matrices to tune stem cell behavior

et al. 2018

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The extracellular matrix (ECM)-biomimetic fibrillar structure of platelet lysate (PL) gels along with their enriched milieu of biomolecules has drawn significant interest in regenerative medicine applications. However, PL-based gels have poor structural stability, which severely limits their performance as a bioinstructive biomaterial. Here, rod-shaped cellulose nanocrystals (CNC) are used as a novel approach to modulate the physical and biochemical microenvironment of PL gels enabling their effective use as injectable human-based cell scaffolds with a level of biomimicry that is difficult to recreate with synthetic biomaterials. The incorporation of CNC (0 to 0.61 wt%) into the PL fibrillar network during the coagulation cascade leads to decreased fiber branching, increased interfiber porosity (from 66 to 83%) and modulates fiber (from 1.4 ± 0.7 to 27 ± 12 kPa) and bulk hydrogel (from 18 ± 4 to 1256 ± 82 Pa) mechanical properties. As a result of these physicochemical alterations, nanocomposite PL hydrogels resist the typical extensive clot retraction (from 76 ± 1 to 24 ± 3 at day 7) and show favored retention of PL bioactive molecules. The feedback of these cues on the fate of human adipose-derived stem cells is evaluated, showing how it can be explored to modulate the commitment of encapsulated stem cells toward different genetic phenotypes without the need for additional external biological stimuli. These fibrillar nanocomposite hydrogels allow therefore the exploration of the outstanding biological properties of human-based PL as an efficient engineered ECM which can be tailored to trigger specific regenerative pathways in minimal invasive strategies.

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Section: Resultsmentioning

confidence: 99%

Human-based fibrillar nanocomposite hydrogels as bioinstructive matrices to tune stem cell behavior

et al. 2018

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“…Next, PL is void of platelet debris and does not form fibrin glue easily, which is known to trap some of the growth factors present in these blood derivatives [96]. Unlike PRP pools, growth factor concentrations from different pools of PL show insignificant variations, contributing to predictable outcomes when used to guide cell behaviour [97]. Both PL and PRP also contain cell adhesion molecules to support cell adhesion to the ECM [98].…”

Section: Molecules Referencesmentioning

confidence: 99%

“…Blood derivatives such as PL not only contain factors that are capable of inducing cell migration, but also stimulants that drive cell differentiation [97]. The most abundant products from the α-granules of platelets are PDGF, IGF and TGF-β, which have been linked to the recruitment of progenitor cells as well as the differentiation of pre-osteoblast cells.…”

Section: Driving Stem Cell Differentiationmentioning

confidence: 99%

“…Additionally, other important factors such as VEGF and BMP are known to be potent inducers of vascularization and bone formation [112]. BMP along with IGF and TGF-β belong to the same superfamily of proteins, which play significant roles in the differentiation of cells to form hard tissue [97,113]. BMPs are perhaps the most popular of them all, widely recognised for their potential in inducing ectotopic osteogenesis, and have received both credit and criticism for their use as a recombinant protein [114][115][116].…”

Section: Driving Stem Cell Differentiationmentioning

confidence: 99%

“…Though each GF mentioned above has been associated with differentiation when isolated, whether the same effects would be observed via blood derivatives is questionable. Nonetheless, it is clear that GFs in blood derivatives provide a nurturing microenvironment for cells, while effectively recruiting progenitor cells [97,122]. Hence, the use of osteoinductive materials in combination with blood derivatives could possibly have synergistic effects towards cell differentiation and periodontal tissue formation [97,117].…”

Section: Driving Stem Cell Differentiationmentioning

confidence: 99%

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Attachment and spatial organisation of human mesenchymal stem cells on poly(ethylene glycol) hydrogels

Chahal

Schweikle

Heyward

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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Strategies that enable hydrogel substrates to support cell attachment typically incorporate either entire extracellular matrix proteins or synthetic peptide fragments such as the RGD (arginine-glycine-aspartic acid) motif. Previous studies have carefully analysed how material characteristics can affect single cell morphologies. However, the influence of substrate stiffness and ligand presentation on the spatial organisation of human mesenchymal stem cells (hMSCs) have not yet been examined. In this study, we assessed how hMSCs organise themselves on soft (E = 7.4-11.2 kPa) and stiff (E = 27.3-36.8 kPa) poly(ethylene glycol) (PEG) hydrogels with varying concentrations of RGD (0.05-2.5 mM). Our results indicate that hMSCs seeded on soft hydrogels clustered with reduced cell attachment and spreading area, irrespective of RGD concentration and isoform. On stiff hydrogels, in contrast, cells spread with high spatial coverage for RGD concentrations of 0.5 mM or higher. In conclusion, we identified that an interplay of hydrogel stiffness and the availability of cell attachment motifs are important factors in regulating hMSC organisation on PEG hydrogels. Understanding how cells initially interact and colonise the surface of this material is a fundamental prerequisite for the design of controlled platforms for tissue engineering and mechanobiology studies.

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Multifunctional Surfaces for Improving Soft Tissue Integration

Vilaça

Domingues

Tiainen

et al. 2021

Adv Healthcare Materials

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Metallic implants are widely used in diverse clinical applications to aid in recovery from lesions or to replace native hard tissues. However, the lack of integration of metallic surfaces with soft tissue interfaces causes the occurrence of biomaterial-associated infections, which can trigger a complicated inflammatory response and, ultimately, implant failure. Here, a multifunctional implant surface showing nanoscale anisotropy, based on the controlled deposition of cellulose nanocrystals (CNC), and biological activity derived from platelet lysate (PL) biomolecules sequestered and presented on CNC surface, is proposed. The anisotropic radial nanopatterns are produced on polished titanium surfaces by spin-coating CNC at high speed. Furthermore, CNC surface chemistry allows to further sequester and form a coating of bioactive molecules derived from PL. The surface anisotropy provided by CNC guides fibroblasts growth and alignment up to 14 days of culture. Moreover, PL-derived biomolecules polarize macrophages toward the M2-like anti-inflammatory phenotype. These results suggest that the developed multifunctional surfaces can promote soft tissue integration to metallic implants and, at the same time, prevent bacterial invasion, tissue inflammation, and failure of biomedical metallic implants.

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Periodontal tissue engineering: current strategies and the role of platelet rich hemoderivatives

Cited by 23 publications

References 115 publications

Human-based fibrillar nanocomposite hydrogels as bioinstructive matrices to tune stem cell behavior

Human-based fibrillar nanocomposite hydrogels as bioinstructive matrices to tune stem cell behavior

Attachment and spatial organisation of human mesenchymal stem cells on poly(ethylene glycol) hydrogels

Multifunctional Surfaces for Improving Soft Tissue Integration

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