Periodontal tissue generation using autologous dental ligament micro-grafts: Case report with 6 months follow-up

Graziano, Antonio; Carinci, Francesco; Scolaro, S; d’Aquino, Riccardo

doi:10.13172/2052-7837-1-2-711

Cited by 36 publications

(49 citation statements)

References 33 publications

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“…This preliminary study showed that micro-grafts obtained by this protocol can be combined with collagen sponges, as already reported in other clinical applications, to optimize the efficacy of micro-grafts implants 9,10 . In particular, this study reported the capacity of bio-complexes, constituted by micro-grafts and collagen sponges, to adjuvant the wound healing of a leg lesion after 30 days from clinical application.…”

Section: Discussionsupporting

confidence: 67%

“…Collect skinsamples of the patient through a biopsy punch ( Figure 1B) and disaggregate adding 1 ml of saline solution for each piece to obtain autologous micro-grafts 6,7,9 (or see step 2.1).…”

Section: Bio-complex Building For Clinical Applicationmentioning

confidence: 99%

“…A case presentation is also herein described as a clinical application of autologous micro-grafts obtained by this protocol in combination with collagen sponges. This approach has already been reported to be efficient in the mechanical disaggregation of human tissues 5 and it has been used clinically for grafts and regeneration of dermal tissues 6,7 as well as for regenerative therapies of connective tissues in oralmaxillofacial surgery [8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

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Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Purpura¹,

Bondioli²,

Graziano³

et al. 2016

JoVE

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Several new methods have been developed in the field of biotechnology to obtain autologous cellular suspensions during surgery, in order to provide one step treatments for acute and chronic skin lesions. Moreover, the management of chronic but also acute wounds resulting from trauma, diabetes, infections and other causes, remains challenging. In this study we describe a new method to create autologous micrografts from cutaneous tissue of a single patient and their clinical application. Moreover, in vitro biological characterization of cutaneous tissue derived from skin, de-epidermized dermis (Ded) and dermis of multi-organ and/or multi-tissue donors was also performed. All tissues were disaggregated by this new protocol, allowing us to obtain viable micro-grafts. In particular, we reported that this innovative protocol is able to create bio-complexes composed by autologous micro-grafts and collagen sponges ready to be applied on skin lesions. The clinical application of autologous bio-complexes on a leg lesion was also reported, showing an improvement of both re-epitalization process and softness of the lesion. Additionally, our in vitro model showed that cell viability after mechanical disaggregation with this system is maintained over time for up to seven (7) days of culture. We also observed, by flow cytometry analysis, that the pool of cells obtained from disaggregation is composed of several cell types, including mesenchymal stem cells, that exert a key role in the processes of tissue regeneration and repair, for their high regenerative potential. Finally, we demonstrated in vitro that this procedure maintains the sterility of micro-grafts when cultured in Agar dishes. In summary, we conclude that this new regenerative approach can be a promising tool for clinicians to obtain in one step viable, sterile and ready to use micrografts that can be applied alone or in combination with most common biological scaffolds. Video LinkThe video component of this article can be found at

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Section: Discussionsupporting

confidence: 67%

Section: Bio-complex Building For Clinical Applicationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Purpura¹,

Bondioli²,

Graziano³

et al. 2016

JoVE

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“…In line with this regenerative potential of the Rigenera protocol, in a previous paper, we demonstrated that micro-grafts combined with collagen sponges to form a bio-complex applied on a leg lesion successfully repaired the lesion, promoting the re-epithelialisation and the softness of the tissue (17). Furthermore, the Rigenera protocol is also successfully applied in the dentistry field where the regeneration of both atrophic maxilla (13) and periodontal tissue (14) was reported and in aesthetic surgery promoting the engraftment of the transplanted hair (15) and the improvement of pathological scars (18). The capacity of these micro-grafts to improve wound healing was also supported by in vitro results showing that these maintain a high cell viability despite the mechanical disaggregation that is performed to obtain them (17,19).…”

Section: Discussionmentioning

confidence: 56%

Rigenera protocol in the treatment of surgical wound dehiscence

Marcarelli¹,

Trovato

Novarese³

et al. 2016

International Wound Journal

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The effective management of post-operative wounds is important to prevent potential complications such as surgical-site infections and wound dehiscence. The purpose of this study was to treat wound dehiscence in elderly patients who were subjected to orthopaedic surgical interventions. The dehisced wounds were treated with autologous micro-grafts obtained using a promising CE-certified medical device called Rigeneracons. This instrument is a biological disruptor of human tissues able to specifically select progenitor cells that, as already reported in previous studies, maintain high cell viability but mainly have a high regenerative potential, allowing the repair of damaged tissues. Autologous micro-grafts obtained by Rigeneracons are ready to use and can be applied alone or in combination with biological scaffolds directly on the injured area. We observed in our patients a complete remission of dehisced wounds, on average, after 30 days from micro-grafts application and a total wound re-epithelialisation after 1 year from the surgical intervention. In conclusion, although we reported only three patients, autologous micro-grafts can be considered a promising approach for the treatment of dehisced wounds, improving the wound-healing process and in general the patient's quality of life without using other dressings.

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“…To this regard, we recently demonstrated the efficacy of Rigeneracons® device in the obtaining progenitors cells from dental pulp (Brunelli et al, ) and adipose tissue recovered from the discard of follicular slicing (Zanzottera et al, ). Furthermore, we showed the Rigeneracons® device was able to provide autologous micro‐grafts from connective tissues to improve the periodontal tissue generation (Graziano et al, ). On the basis of these evidences, we aimed to demonstrate in vitro the capacity of Rigeneracons® medical device to provide, from three different samples of human tissues, a high percentage of viable progenitors cells.…”

mentioning

confidence: 99%

A New Medical Device Rigeneracons Allows to Obtain Viable Micro‐Grafts From Mechanical Disaggregation of Human Tissues

Trovato

Monti

Fante

et al. 2015

Journal Cellular Physiology

107

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Autologous graft is considered the gold standard of graft materials; however, this approach is still limited due to both small amount of tissue that can be collected and to reduced cell viability of cells that can be obtained. The aim of this preliminary study was to demonstrate the efficacy of an innovative medical device called Rigeneracons® (CE certified Class I) to provide autologous micro-grafts immediately available to be used in the clinical practice. Moreover, Rigeneracons® is an instrument able to create micro-grafts enriched of progenitors cells which maintain their regenerative and differentiation potential. We reported preliminary data about viability cell of samples derived from different kind of human tissues, such as periosteum, cardiac atrial appendage biopsy, and lateral rectus muscle of eyeball and disaggregated by Rigeneracons®. In all cases we observed that micro-grafts obtained by Rigeneracons® displayed high cell viability. Furthermore, by cell characterization of periosteum samples, we also evidenced an high positivity to mesenchymal cell markers, suggesting an optimal regenerative potential.

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Periodontal tissue generation using autologous dental ligament micro-grafts: Case report with 6 months follow-up

Cited by 36 publications

References 33 publications

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Rigenera protocol in the treatment of surgical wound dehiscence

A New Medical Device Rigeneracons Allows to Obtain Viable Micro‐Grafts From Mechanical Disaggregation of Human Tissues

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