Periostin Expression is Altered in Aortic Valves in Smad6 Mutant Mice

Sugi, Yukiko; Kern, Michael J.; Markwald, Roger R.; Burnside, Jessica L.

doi:10.4172/2167-0897.1000101

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…Because the QRT-PCR primers we used for the present study are not isoform specific but designed to detect all isoforms of chick versican it is of future interest to examine whether BMP-2 induces chick versican mRNA in an isoform-specific manner in post-EMT AV CMCs. In addition, because our mouse developmental data indicate that versican protein expression and HA deposition remain intense in all four cardiac valves at the newborn stage [40], subsequent investigations are needed to determine whether HA and versican play significant roles in later valve leaflet formation and stratification of mature valves. …”

Section: Discussionmentioning

confidence: 99%

BMP-2 Induces Versican and Hyaluronan That Contribute to Post-EMT AV Cushion Cell Migration

et al. 2013

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Distal outgrowth and maturation of mesenchymalized endocardial cushions are critical morphogenetic events during post-EMT atrioventricular (AV) valvuloseptal morphogenesis. We explored the role of BMP-2 in the regulation of valvulogenic extracellular matrix (ECM) components, versican and hyaluronan (HA), and cell migration during post-EMT AV cushion distal outgrowth/expansion. We observed intense staining of versican and HA in AV cushion mesenchyme from the early cushion expansion stage, Hamburger and Hamilton (HH) stage-17 to the cushion maturation stage, HH stage-29 in the chick. Based on this expression pattern we examined the role of BMP-2 in regulating versican and HA using 3D AV cushion mesenchymal cell (CMC) aggregate cultures on hydrated collagen gels. BMP-2 induced versican expression and HA deposition as well as mRNA expression of versican and Has2 by CMCs in a dose dependent manner. Noggin, an antagonist of BMP, abolished BMP-2-induced versican and HA as well as mRNA expression of versican and Has2. We further examined whether BMP-2-promoted cell migration was associated with expression of versican and HA. BMP-2- promoted cell migration was significantly impaired by treatments with versican siRNA and HA oligomer. In conclusion, we provide evidence that BMP-2 induces expression of versican and HA by AV CMCs and that these ECM components contribute to BMP-2-induced CMC migration, indicating critical roles for BMP-2 in distal outgrowth/expansion of mesenchymalized AV cushions.

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Section: Discussionmentioning

confidence: 99%

BMP-2 Induces Versican and Hyaluronan That Contribute to Post-EMT AV Cushion Cell Migration

et al. 2013

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“…TGF-β/ Smads signaling pathway is involved in cardiac development, and POSTN is the downstream response factor. That exogenous treatment of primary cardiac broblasts and vascular smooth muscle cells (VSMCs) with recombinant TGF-β1 promoted the expression of Periostin via canonical SMADdependent signaling [14,15]. Periostin -/mice were susceptible to matrix strati cation disorder, reduced TGF signal transduction, protein-polysaccharide aggregation, discontinuous valve lobules, and delamination defects [16].…”

Section: Discussionmentioning

confidence: 99%

Gravida Serum POSTN and PAPPA as the Potential Biomarkers for Fetal Congenital Heart Disease Based on Quantitative and Qualitative Proteomics

Xia

Cheng

Duan

et al. 2021

Preprint

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Background: Fetal congenital heart disease is the most common congenital defect worldwide. It has some missed diagnoses and a lack of disease biomarkers. We aim to seek objective biomarkers for noninvasive prenatal diagnosis of fetal congenital heart disease to reduce the missed diagnosis and explore its mechanism,Methods: This study used data-independent acquisition and parallel reaction monitoring to explore potential protein biomarkers that co-exist in gravida serum and fetal amniotic fluid. Moreover, logistic regression and ROC curve to establish the diagnostic model of fetal congenital heart disease potential biomarkers and molecular biology experiments were performed to validate proteomics results and explore the mechanism. Results: Proteomics and bioinformatics results show that 12 proteins co-exist in gravida serum and amniotic fluid. We identified POSTN and PAPPA in GS as candidate biomarkers and established the diagnostic model with a sensitivity of 100%, a specificity of 95% and the AUC value of 0.968 to diagnose congenital heart disease. In addition, the results of ELISA, IHC, and RT-PCR were consistent with those of proteomics. Moreover, POSTN may involve in fetal congenital heart disease through the TGF-beta signaling pathway. Conclusion: It is the first time to find that POSTN and PAPPA in GS are related to fetal congenital heart disease, contributing to developing a novel noninvasive prenatal method to diagnose fetal congenital heart disease and reduce congenital disabilities.

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“…ECM components are deposited in the maturing AV endocardial cushions and reported to play significant roles in valvulogenesis and valve disease development (Review: de Vlaming et al ., 2012; Camenisch et al ., 2000; Camenisch et al ., 2002; Dupuis et al ., 2011; Hinton et al ., 2006; Hinton and Yutzey, 2011; Lincoln et al ., 2006b; Norris et al ., 2008; Peacock et al ., 2008; Sugi et al ., 2012; Yamamura et al ., 1997). Furthermore, our previous work with 3-D chick AV cushion mesenchymal cell aggregate cultures has indicated that BMP2 induces mRNA expression and deposition of ECM components, periostin (Inai et al ., 2008), hyaluronan and versican (Inai et al ., 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Anti-mouse periostin antibodies were a kind gift from Drs. Hoffman and Krug (Medical University of South Carolina) and immunohistochemistry was performed as previously described (Sugi et al ., 2012). For the secondary antibody, either fluorescein conjugated goat anti-mouse IgG (MP Biomedicals, #55514) or -rabbit IgG (MP Biomedicals, # 55662) was used.…”

Section: Methodsmentioning

confidence: 99%

BMP2 expression in the endocardial lineage is required for AV endocardial cushion maturation and remodeling

Saxon

Baer

Barton

et al. 2017

Developmental Biology

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Distal outgrowth, maturation and remodeling of the endocardial cushion mesenchyme in the atrioventricular (AV) canal are the essential morphogenetic events during four-chambered heart formation. Mesenchymalized AV endocardial cushions give rise to the AV valves and the membranous ventricular septum (VS). Failure of these processes results in several human congenital heart defects. Despite this clinical relevance, the mechanisms governing how mesenchymalized AV endocardial cushions mature and remodel into the membranous VS and AV valves have only begun to be elucidated. The role of BMP signaling in the myocardial and secondary heart forming lineage has been well studied; however, little is known about the role of BMP2 expression in the endocardial lineage. To fill this knowledge gap, we generated Bmp2 endocardial lineage-specific conditional knockouts (referred to as Bmp2 cKOEndo) by crossing conditionally-targeted Bmp2flox/flox mice with a Cre-driver line, Nfatc1Cre, wherein Cre-mediated recombination was restricted to the endocardial cells and their mesenchymal progeny. Bmp2 cKOEndo mouse embryos did not exhibit failure or delay in the initial AV endocardial cushion formation at embryonic day (ED) 9.5–11.5; however, significant reductions in AV cushion size were detected in Bmp2 cKOEndo mouse embryos when compared to control embryos at ED13.5 and ED16.5. Moreover, deletion of Bmp2 from the endocardial lineage consistently resulted in membranous ventricular septal defects (VSDs), and mitral valve deficiencies, as evidenced by the absence of stratification of mitral valves at birth. Muscular VSDs were not found in Bmp2 cKOEndo mouse hearts. To understand the underlying morphogenetic mechanisms leading to a decrease in cushion size, cell proliferation and cell death were examined for AV endocardial cushions. Phospho-histone H3 analyses for cell proliferation and TUNEL assays for apoptotic cell death did not reveal significant differences between control and Bmp2 cKOEndo in AV endocardial cushions. However, mRNA expression of the extracellular matrix components, versican, Has2, collagen 9a1, and periostin was significantly reduced in Bmp2 cKOEndo AV cushions. Expression of transcription factors implicated in the cardiac valvulogenesis, Snail2, Twist1 and Sox9, was also significantly reduced in Bmp2 cKOEndo AV cushions. These data provide evidence that BMP2 expression in the endocardial lineage is essential for the distal outgrowth, maturation and remodeling of AV endocardial cushions into the normal membranous VS and the stratified AV valves.

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Periostin Expression is Altered in Aortic Valves in Smad6 Mutant Mice

Cited by 6 publications

References 46 publications

BMP-2 Induces Versican and Hyaluronan That Contribute to Post-EMT AV Cushion Cell Migration

BMP-2 Induces Versican and Hyaluronan That Contribute to Post-EMT AV Cushion Cell Migration

Gravida Serum POSTN and PAPPA as the Potential Biomarkers for Fetal Congenital Heart Disease Based on Quantitative and Qualitative Proteomics

BMP2 expression in the endocardial lineage is required for AV endocardial cushion maturation and remodeling

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