Periostin facilitates eosinophil tissue infiltration in allergic lung and esophageal responses

Blanchard, Carine; Mingler, M K; McBride, Melissa; Putnam, Philip E.; Collins, Margaret H.; Chang, Geen-Dong; Stringer, Keith; Abonia, J. Pablo; Molkentin, Jeffery D.; Rothenberg, Marc E.

doi:10.1038/mi.2008.15

Cited by 246 publications

(247 citation statements)

References 44 publications

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“…Accordingly, recombinant periostin was found to directly enhance adhesion of eosinophils and monocytes to fibronectin and function as a chemoattractant to primary monocytes and macrophages in migration assays. 24,25 Chronic inflammation plays a central role in development of fibrosis and kidney damage. Concomitantly to the attenuation of inflammation, periostin deficiency diminished the expression of collagens and TGF-b1, and improved renal structure and function.…”

Section: Discussionmentioning

confidence: 99%

NFκB-Induced Periostin Activates Integrin-β3 Signaling to Promote Renal Injury in GN

Prakoura

Kavvadas

Kormann

et al. 2016

JASN

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De novo expression in the kidney of periostin, a protein involved in odontogenesis and osteogenesis, has been suggested as a biomarker of renal disease. In this study, we investigated the mechanism(s) of induction and the role of periostin in renal disease. Using a combination of bioinformatics, reporter assay, and chromatin immunoprecipitation analyses, we found that NFkB and other proinflammatory transcription factors induce periostin expression in vitro and that binding of these factors on the periostin promoter is enriched in glomeruli during experimental GN. Mice lacking expression of periostin displayed preserved renal function and structure during GN. Furthermore, delayed administration of periostin antisense oligonucleotides in wild-type animals with GN reversed already established proteinuria, diminished tissue inflammation, and improved renal structure. Lack of periostin expression also blunted the de novo renal expression of integrin-b3 and phosphorylation of focal adhesion kinase and AKT, known mediators of integrin-b3 signaling that affect cell motility and survival, observed during GN in wild-type animals. In vitro, recombinant periostin increased the expression of integrin-b3 and the concomitant phosphorylation of focal adhesion kinase and AKT in podocytes. Notably, periostin and integrin-b3 were highly colocalized in biopsy specimens from patients with inflammatory GN. These results demonstrate that interplay between periostin and renal inflammation orchestrates inflammatory and fibrotic responses, driving podocyte damage through downstream activation of integrin-b3 signaling. Targeting periostin may be a novel therapeutic strategy for treating CKD.

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Section: Discussionmentioning

confidence: 99%

NFκB-Induced Periostin Activates Integrin-β3 Signaling to Promote Renal Injury in GN

Prakoura

Kavvadas

Kormann

et al. 2016

JASN

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“…Although periostin has been considered a mediator of inflammation and fibrosis in allergic diseases, these studies have focused on periostin derived from fibroblasts (22,23); to our knowledge, the expression of periostin by epithelial cells has not been considered in mechanisms of airway fibrosis in asthma.…”

Section: Mmp-9mentioning

confidence: 99%

Roles of epithelial cell-derived periostin in TGF-β activation, collagen production, and collagen gel elasticity in asthma

Sidhu

Yuan

Innes

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

356

303

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Periostin is considered to be a matricellular protein with expression typically confined to cells of mesenchymal origin. Here, by using in situ hybridization, we show that periostin is specifically up-regulated in bronchial epithelial cells of asthmatic subjects, and in vitro, we show that periostin protein is basally secreted by airway epithelial cells in response to IL-13 to influence epithelial cell function, epithelial-mesenchymal interactions, and extracellular matrix organization. In primary human bronchial epithelial cells stimulated with periostin and epithelial cells overexpressing periostin, we reveal a function for periostin in stimulating the TGF-β signaling pathway in a mechanism involving matrix metalloproteinases 2 and 9. Furthermore, conditioned medium from the epithelial cells overexpressing periostin caused TGF-β-dependent secretion of type 1 collagen by airway fibroblasts. In addition, mixing recombinant periostin with type 1 collagen in solution caused a dramatic increase in the elastic modulus of the collagen gel, indicating that periostin alters collagen fibrillogenesis or cross-linking and leads to stiffening of the matrix. Epithelial cell-derived periostin in asthma has roles in TGF-β activation and collagen gel elasticity in asthma.airway fibrosis | fibroblasts | epithelial to mesenchymal transition | MMP-2 | MMP-9

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“…These inflammatory mediators are known to induce various effects such as up‐regulation of adhesion molecules to modulate cellular trafficking and recruitment, mucus secretion, tissue remodeling, and impairment of barrier integrity,24, 25 which can contribute to disease progression. For example, eosinophil‐derived TGF‐β is linked to tissue remodeling and induction of the extracellular matrix protein periostin in patient biopsy specimens which increases eosinophil infiltration in the mucosal layer, thus facilitating disease pathogenesis 26, 27. Furthermore, as indicated above, granule proteins such as eosinophil cationic protein, Epx, and major basic protein released by activated eosinophils during inflammation can contribute to tissue damage (alter barrier function) and dysfunction (diarrhea with bleeding) 9, 10, 11, 12.…”

Section: Discussionmentioning

confidence: 99%

Assessment of eosinophils in gastrointestinal inflammatory disease of dogs

Baştan

Seelig

Day

et al. 2018

Veterinary Internal Medicne

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BackgroundAccurate identification of eosinophils in the gastrointestinal (GI) tract of dogs with eosinophilic GI disease (EGID) by histological evaluation is challenging. The currently used hematoxylin and eosin (H&E) staining method detects intact eosinophils but does not detect degranulated eosinophils, thus potentially underrepresenting the number of infiltrating eosinophils.ObjectiveTo develop a more sensitive method for identifying and quantifying both intact and degranulated eosinophils to diagnose EGID more accurately.MethodsEndoscopically obtained paraffin‐embedded intestinal biopsy specimens from dogs with GI signs were examined. The study groups were dogs with eosinophilic enteritis (EE), lymphoplasmacytic and mixed enteritis, and control dogs with GI signs but no histologic changes on tissue sections. Consecutive sections were immunolabeled with monoclonal antibodies (mAbs) against the eosinophil granule protein eosinophil peroxidase (Epx) and stained by H&E, respectively. The number of eosinophils was manually quantified and classified as intact or degranulated.ResultsThe number of intact eosinophils detected in Epx mAb‐labeled duodenal sections was significantly higher compared with that in H&E‐stained sections, with a similar relationship noted in the colon and stomach. The Epx mAb allowed the unique assessment of eosinophil degranulation. The number of intact and degranulated eosinophils was significantly higher in duodenal lamina propria of the EE and mixed group compared to the control group.ConclusionImmunohistochemical detection of Epx provides a more precise method to detect GI tract eosinophils compared to H&E staining and could be used as an alternative and reliable diagnostic tool for assessment of biopsy tissues from dogs with EGID.

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Periostin facilitates eosinophil tissue infiltration in allergic lung and esophageal responses

Cited by 246 publications

References 44 publications

NFκB-Induced Periostin Activates Integrin-β3 Signaling to Promote Renal Injury in GN

NFκB-Induced Periostin Activates Integrin-β3 Signaling to Promote Renal Injury in GN

Roles of epithelial cell-derived periostin in TGF-β activation, collagen production, and collagen gel elasticity in asthma

Assessment of eosinophils in gastrointestinal inflammatory disease of dogs

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