Peripheral Blood-Based Biopsy for Breast Cancer Risk Prediction and Early Detection

Nassar, Farah; Chamandi, Ghada; Tfaily, Mohamad Ali; Zgheib, Nathalie K.; Nasr, Rihab

doi:10.3389/fmed.2020.00028

Cited by 30 publications

(20 citation statements)

References 80 publications

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“…Mammography-based screening significantly reduces BC-related mortality, but intrinsic and practical limitations call for novel screening approaches [ 17 – 20 ]. Blood-based biomarkers exploiting CTC, cancer-derived DNA or RNA, are being explored, but so far none reached clinical routine practice [ 22 , 43 ]. Similarly, there are no validated biomarkers for monitoring patients’ response to treatment or detecting relapses before they become symptomatic.…”

Section: Discussionmentioning

confidence: 99%

Circulating immune cell populations related to primary breast cancer, surgical removal, and radiotherapy revealed by flow cytometry analysis

Cattin

Fellay

Calderoni³

et al. 2021

Breast Cancer Res

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Background Advanced breast cancer (BC) impact immune cells in the blood but whether such effects may reflect the presence of early BC and its therapeutic management remains elusive. Methods To address this question, we used multiparametric flow cytometry to analyze circulating leukocytes in patients with early BC (n = 13) at the time of diagnosis, after surgery, and after adjuvant radiotherapy, compared to healthy individuals. Data were analyzed using a minimally supervised approach based on FlowSOM algorithm and validated manually. Results At the time of diagnosis, BC patients have an increased frequency of CD117+CD11b+ granulocytes, which was significantly reduced after tumor removal. Adjuvant radiotherapy increased the frequency of CD45RO+ memory CD4+ T cells and CD4+ regulatory T cells. FlowSOM algorithm analysis revealed several unanticipated populations, including cells negative for all markers tested, CD11b+CD15low, CD3+CD4−CD8−, CD3+CD4+CD8+, and CD3+CD8+CD127+CD45RO+ cells, associated with BC or radiotherapy. Conclusions This study revealed changes in blood leukocytes associated with primary BC, surgical removal, and adjuvant radiotherapy. Specifically, it identified increased levels of CD117+ granulocytes, memory, and regulatory CD4+ T cells as potential biomarkers of BC and radiotherapy, respectively. Importantly, the study demonstrates the value of unsupervised analysis of complex flow cytometry data to unravel new cell populations of potential clinical relevance.

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Section: Discussionmentioning

confidence: 99%

Circulating immune cell populations related to primary breast cancer, surgical removal, and radiotherapy revealed by flow cytometry analysis

Cattin

Fellay

Calderoni³

et al. 2021

Breast Cancer Res

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show abstract

“…In recent years, the incidence of BC has continued to rise. This is a heavy burden for some developing countries, in which screening and awareness programs are seriously lacking ( Nassar et al, 2020 ). BC is usually called “stone breast” in TCM theory, and in which the pathogenesis of BC is considered to be related to the intermingling of factors such as emotional disorder, Liver-kidney Yin Deficiency, Qi stagnation and blood stasis, and irregular diet ( Xie and Long, 2019 ; Xu and Luo, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Efficacy and Safety of Chinese Herbal Injections Combined With Cyclophosphamide and 5-Fluorouracil Chemotherapies in Treatment of Breast Cancer: A Bayesian Network Meta-Analysis

Wang

et al. 2021

Front. Pharmacol.

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Background: Given the limitations of chemotherapy for the treatment of breast cancer (BC) and the wide exploration of Chinese herbal injections (CHIs), this network meta-analysis (NMA) was conducted to analyze the comparative efficacy and safety of nine CHIs combined with CF (Cyclophosphamide and 5-Fluorouracil) chemotherapy regimens in the treatment of BC.Methods: Several electronic databases were searched to identify randomized controlled trials (RCTs) from inception to January 6, 2020. RCTs were screened by pre-established eligibility criteria, and the quality of which was assessed using the Cochrane risk of bias tool. Outcomes such as the clinical effectiveness rate, performance status, peripheral hemogram, and detection of T-lymphocyte subsets were analyzed using the Winbugs 1.4.3 and Stata 13.0 software. Surface under the cumulative ranking curve (SUCRA) probability values were applied to rank the examined treatments. Cluster analysis was performed to compare the effect of CHIs between two or three different outcomes.Results: A total of 84 RCTs involving 7855 patients and nine CHIs were included. The results showed that compared to CF chemotherapy regimens alone, the ones injected along with Aidi, Shenmai, Shenqi Fuzheng, Kangai, Kanglaite, or Shengmai combined with CF can improve the clinical effectiveness rate. Aidi, Shenmai, Shenqi Fuzheng, Compound Kushen, Kangai, and Kanglaite injection combined with CF can improve the performance status. Shenqi Fuzheng injection was considered as a favorable choice for relieving adverse reactions. According to the results of cluster analysis, Aidi injection and Compound Kushen injection plus CF were more favorable for the clinical effectiveness rate and performance status.Conclusion: In conclusion, Shenqi Fuzheng, Compound Kushen, Aidi, and Kangai injection combined with CF chemotherapy regimen have more significant effects for patients with BC. However, more high-quality clinical RCTs, especialy which correctly use blinding and allocation concealment, are required to support the conclusions.

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“…Mammography-based screening signi cantly reduces BC-related mortality, but intrinsic and practical limitations call for novel screening approaches (17,19,20,42). Blood based biomarkers exploiting cancerderived CTC, DNA or RNA are being explored, but so far none reached clinical routine practice (22,43).…”

Section: Discussionmentioning

confidence: 99%

Circulating Immune Cell Populations Related to Primary Breast Cancer, Surgical Removal and Radiotherapy Revealed by Flow Cytometry Analysis

Cattin

Fellay

Calderoni³

et al. 2021

Preprint

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Background: Advanced breast cancer (BC) impact immune cells in the blood but whether such effects may reflect the presence of early BC and its therapeutic management remains elusive. Methods: To address this question, we used multiparametric flow cytometry to analyse circulating leukocytes in patients with early BC (n=13) at time of diagnosis, after surgery and after adjuvant radiotherapy, compared to healthy individuals. Data were analysed using a minimally supervised approach based on FlowSOM algorithm and validated manually. Results: At time of diagnosis, BC patients have an increased frequency of CD117+ Granulocytic-Myeloid Derived Suppressor Cells (G-MDSC), which was significantly reduced after tumor removal. Adjuvant radiotherapy increased the frequency of CD45RO+ memory CD4+ T cells and CD4+ regulatory T cells. FlowSOM algorithm analysis revealed several unanticipated populations, including cells negative for all markers tested, CD11b+CD15low, CD3+CD4-CD8-, CD3+CD4+CD8+, CD3+CD8+CD127+CD45RO+ cells, associated with BC or radiotherapy. Conclusions: This study revealed changes in blood leukocytes associated with primary BC, surgical removal and adjuvant radiotherapy. Specifically, it identified increased levels of CD117+ G-MDSC, memory and regulatory CD4+ T cells as potential biomarkers of BC and radiotherapy, respectively. Importantly, the study demonstrates the value of unsupervised analysis of complex flow cytometry data to unravel new cell populations of potential clinical relevance.

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Peripheral Blood-Based Biopsy for Breast Cancer Risk Prediction and Early Detection

Cited by 30 publications

References 80 publications

Circulating immune cell populations related to primary breast cancer, surgical removal, and radiotherapy revealed by flow cytometry analysis

Circulating immune cell populations related to primary breast cancer, surgical removal, and radiotherapy revealed by flow cytometry analysis

Comparative Efficacy and Safety of Chinese Herbal Injections Combined With Cyclophosphamide and 5-Fluorouracil Chemotherapies in Treatment of Breast Cancer: A Bayesian Network Meta-Analysis

Circulating Immune Cell Populations Related to Primary Breast Cancer, Surgical Removal and Radiotherapy Revealed by Flow Cytometry Analysis

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