Peripheral circulating insulin-like growth factor-I and -II in cattle

Nikolić, Jasmina B.; Nedić, Olgica; Šamanc, Horea; Aleksić, S.; Miscevic, B.; Kulcsár, Margit

doi:10.1556/004.49.2001.1.7

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…Leptin was determined by a ruminant-specific, homologous, double-antibody, non-equilibrium 125 I-RIA method (Delavaud et al, 2002) modified and validated for bovine plasma in our lab (assay sensitivity: 0.032 nmol/l; intra-and interassay CVs: 4.6-10.1% and 5.6-12.2%, respectively; Kulcsar et al, 2006). Plasma cortisol was determined by a direct 3 H-RIA method developed for human (Csernus, 1982) and equine (Nagy et al, 1998) samples and validated for bovine plasma without modification (sensitivity: 0.26 nmol/l, intra-and interassay CVs: ≤ 5.6% and ≤ 9.5%, respectively; Nikolic et al, 1998;Janosi et al, 2003).…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Interrelationships of growth hormone AluI polymorphism, insulinresistance, milk production and reproductive performance in Holstein-Friesian cos

et al. 2008

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Healthy multiparous Holstein-Friesian cows (n = 22, parity: 2-4) from a large-scale dairy herd in Hungary were subjected to an intravenous glucose tolerance test 10-15 days after calving. AluI genotype of growth hormone, several plasma metabolites and metabolic hormones were determined, and current and previous lactation yields were recorded. We also used the Revised Quantitative Insulin Sensitivity Check Index (RQUICKI) and its modified version (RQUICKI BHB ) for the estimation of peripheral insulin sensitivity. The majority of cows (n = 18) was leucine homozygous (LL), four were heterozygous (LV) and there were no valine homozygous (VV) animals in the population. Current average milk production was not different between AluI genotypes, but LV cows tended to have higher 305-day previous lactation yields (P = 0.13). AluI polymorphism was not associated with any of the calculated glucose and leptin parameters of the intravenous glucose tolerance test (P > 0.58). Heterozygous cows were prone to higher basal insulin levels (P = 0.064), longer time to reach half of the maximal and basal insulin concentrations (P = 0.035 and P = 0.054, respectively) and larger insulin area under the curve (P = 0.032). Both RQUICKI and RQUICKI BHB estimated decreased insulin sensitivity in LV compared to LL cows (P = 0.055 and P = 0.044, respectively). Higher plasma NEFA and BHB levels accounted for slower glucose disappearance and lower insulin release and insulin clearance rate (P < 0.05). Average yield was inversely related to glucose area under the curve (P = 0.040) and time to reach baseline concentration (P = 0.005). Plasma cortisol lowered glucose clearance rate (P = 0.040) and prolonged time to reach basal levels (P = 0.006). More weight loss was associated with higher glucose peak and prolonged glucose disappearance time (P = 0.055 and P = 0.024, respectively). All cows became cyclic and showed signs of estrus during the study period. There were no differences between leucine homozygous and heterozygous animals in the onset of ovarian activity and in the time of first observed estrus (P > 0.540). We conclude that Holstein-Friesian cows heterozygous for AluI polymorphism of the growth hormone gene may be more likely to develop insulin resistance during early lactation than leucine homozygous cows. Decreased insulin sensitivity could be part of a homeorhetic adaptation process that supports nutrient partioning for the use of the mammary gland and may allow LV cows to reach higher yields throughout lactation.

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Section: Laboratory Proceduresmentioning

confidence: 99%

Interrelationships of growth hormone AluI polymorphism, insulinresistance, milk production and reproductive performance in Holstein-Friesian cos

et al. 2008

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“…Neutralised acid-ethanol extracts of plasma were analysed for IGF-1 using a heterologous 125 I-RIA system modified for bovine samples (Nikolic et al, 2001), after validating this assay for ovine plasma without any further changes (sensitivity: 0.20 nmol/l, intra-and interassay CVs: ≤ 6.2 and ≤ 12.0%). The other laboratory procedures were based on the use of commercially available kits (Table 1).…”

Section: Handling Of Samples Laboratory Procedures Statisticsmentioning

confidence: 99%

Endocrine characteristics of late pregnant hyperketonaemic ewes and their reproductive performance following the induction of ovarian cyclicity out of the breeding season

Kulcsár¹,

Dankó²,

Delavaud³

et al. 2006

Acta Veterinaria Hungarica

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Ketosis was diagnosed in a flock of Merino ewes that conceived from synchronised oestrus in the early autumn period. On day 140 of pregnancy the ewes were sampled for determination of βOH-butyrate (BHB), AST, glucose, nonesterified fatty acids (NEFA), total cholesterol (TCH), insulin, T 4 , T 3 , cortisol, IGF-1 and leptin. The results were evaluated according to the number of fetuses born some days later and the presence of hyperketonaemia (BHB: ≥ 1.60 mmol/l). In May, about 3 months after lambing, cyclic ovarian function was induced (Cronolone + eCG), and the ewes were inseminated artificially (AI) 48 h after the removal of gestagen-containing sponge. At the time of AI and 10 days later blood samples were collected again to check the plasma levels of the same constituents as previously (in samples taken at AI), and to monitor the ovarian response by assaying progesterone (in both samples). On day 140 of gestation significantly lower BHB levels were detected in dams with single (n = 41) than in those with twin (n = 57) pregnancies. Hyperketonaemia was found only in ewes bearing twins (n = 27). These animals had higher NEFA and cortisol, and lower TCH, insulin, IGF-1, leptin and T 3 levels than their normoketonaemic twin-bearing flockmates, and those with single pregnancy. The blood glucose concentrations varied within a wide range, and the means of groups did not exhibit any significant differences. The formerly hyperketonaemic individuals were characterised by lower leptin level 3 months after lambing, and they showed a poorer response to the cycle-induction procedure than the others. The non-responders had lower IGF-1 and leptin levels than those ovulated after this treatment. It was concluded that the subclinical form of ovine ketosis is characterised by complex endocrine altera-

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“…The insulin assay was calibrated with bovine (b) insulin (WHO 83/511) and included b‐insulin working standards. Neutralized acid–ethanol extracts of plasma were assayed for IGF‐I and IGF‐II, using classical competitive binding systems, as modified for bovine samples (Nikolić et al, 2001a). In the IGF‐I assay h‐IGF‐II (4 ng/tube) was added to take up any interfering residual binding proteins.…”

Section: Analysesmentioning

confidence: 99%

“…In the IGF‐I assay h‐IGF‐II (4 ng/tube) was added to take up any interfering residual binding proteins. Partially purified b‐IGF‐II was used as the working standard in the IGF‐II assay (Nikolić et al, 2001a). As the assay was calibrated against reference h‐IGF‐II (WHO 96/538), the results are expressed as human equivalents (HE).…”

Section: Analysesmentioning

confidence: 99%

Periparturient Endocrine and Metabolic Changes in Healthy Cows and in Cows Affected by Mastitis

Nikolić

Kulcsár

Kátai

et al. 2003

Journal of Veterinary Medicine Series A

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Transition from pregnancy to lactation in dairy cows involves considerable metabolic adaptation. Additional stress is incurred during infections such as periparturient mastitis. Multiparous Holstein-Friesian cows kept under normal production conditions (n = 15) were used to evaluate changes in circulating metabolite and hormone concentrations from 5 days before to 5 days after calving. Insulin-like growth factor binding protein (IGFBP) profiles were also monitored. Marked time-related changes were observed for plasma thyroid hormone, IGF, cortisol, insulin, beta-hydroxybutyrate (BHB) and non-esterified fatty acid (NEFA) concentrations but not for plasma leptin. A decrease in IGF-II concentration and maximal intensity of the putative IGFBP-1 band occurred at parturition. When compared with the five healthy cows,low IGF-II levels were prolonged to day 2 post-partum in five cows with Escherichia coli-associated mastitis. However, marked decreases in IGFBP-2 band intensity were evident only in two of the four cases examined. Individual total ligand (IGF-I + IGF-II) concentration and IGFBP pattern prepartum were largely regained 5 days post-partum in all cows. Hormone and metabolite concentrations in the two cows with Staphylococcus aureus-associated mastitis were very similar to those in the five healthy cows. Plasma thyroxine (T4) was lower 2 days prepartum in the cows, which later developed Gram-negative mastitis. Multiregression analysis showed that variance in T4 concentration was significantly and independently associated with triiodothyronine (T3) and IGF-I positively and with cortisol negatively (R2 = 0.648). This study confirms the close inter-relationship between the thyroid hormone and IGF axes in cattle and indicates possible effects of Gram-negative mastitis infection on IGF-II metabolism.

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Peripheral circulating insulin-like growth factor-I and -II in cattle

Cited by 6 publications

References 24 publications

Interrelationships of growth hormone AluI polymorphism, insulinresistance, milk production and reproductive performance in Holstein-Friesian cos

Interrelationships of growth hormone AluI polymorphism, insulinresistance, milk production and reproductive performance in Holstein-Friesian cos

Endocrine characteristics of late pregnant hyperketonaemic ewes and their reproductive performance following the induction of ovarian cyclicity out of the breeding season

Periparturient Endocrine and Metabolic Changes in Healthy Cows and in Cows Affected by Mastitis

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