Peripheral Neuron Survival and Outgrowth on Graphene

Convertino, Domenica; Luin, Stefano; Marchetti, Laura; Coletti, Camilla

doi:10.3389/fnins.2018.00001

Cited by 150 publications

(249 citation statements)

References 57 publications

Supporting

Mentioning

227

Contrasting

Order By: Relevance

“…No signiﬁcant influence of household preferences in the higher food aversion in ASD group than sibling groups Thullen 2017 43 62% of participants report at least one FS behavior as problematic.No significant association between FS and parenting stress. Association between child disruptive behavior at mealtime and quality of co-parenting ( p <0.05) Berding 2018 17 Lower rate of exclusive breastfeeding (20%, p<0.05) Higher percentage of children eating less than 20 foods (54%, p <0.05). No differences in picky eating and repetitive eating pattern.…”

Section: Resultsmentioning

confidence: 99%

“… 17 , 20 , 33 , 34 , 36 , 34 , 42 – 49 Most studies found a higher prevalence of FS in ASD children compared with TD controls. 17 , 32 , 36 , 40 50 – 56 However, one study found that a difference between ASD and TD children exists only when considering severe eating selectiveness, while no difference emerged about EP of mild severity. 41…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

<p>Eating and Mealtime Behaviors in Patients with Autism Spectrum Disorder: Current Perspectives</p>

Margari¹,

Marzulli²,

Gabellone³

et al. 2020

NDT

View full text Add to dashboard Cite

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impaired social and communication skills and repetitive and restrictive behaviors. Children and adolescents with ASD are more likely to present feeding problems than their typically developing peers are. The present narrative review of literature aims to highlight the most recent evidence about epidemiology and presentations of eating and mealtime behavioral abnormalities in ASD from infancy to adolescence. Abnormalities in breastfeeding and acceptance of complementary foods have been described by most of the studies evaluating ASD early feeding history. Among the various eating and mealtime behaviors identified in ASD children and adolescents, the most common was food selectivity. The present review also provides brief overviews of the various aspects of food that may influence food acceptance by ASD patients and of the correlation between eating problems and ASD core symptoms, as well as with cognitive level, language skills, and family environment. However, studies evaluating eating problems in ASD children and adolescents are very heterogeneous and they show methodological differences. Moreover, the absence of unique definitions of eating and mealtime behaviors in ASD further limits the comparability of studies.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

<p>Eating and Mealtime Behaviors in Patients with Autism Spectrum Disorder: Current Perspectives</p>

Margari¹,

Marzulli²,

Gabellone³

et al. 2020

NDT

View full text Add to dashboard Cite

show abstract

“…Convertino presented a surface analysis with PLL, PDL and collagen layers, performed by atomic force microscope. Polylysine modified surface is more homogeneous, while network aggregates can be observed in the collagen coating [45]. Therefore, it can be assumed that the heterogeneity of the surface affects the stronger attachment of cells to the surface.…”

Section: Discussionmentioning

confidence: 99%

PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions

Wiatrak

Kubis-Kubiak

Piwowar

et al. 2020

Cells

226

126

View full text Add to dashboard Cite

The PC12 cell line is one of the most commonly used in neuroscience research, including studies on neurotoxicity, neuroprotection, neurosecretion, neuroinflammation, and synaptogenesis. Two types of this line are available in the ATCC collection: traditional PC12 cells grown in suspension and well-attached adherent phenotype. PC12 cells grown in suspension tend to aggregate and adhere poorly to non-coated surfaces. Therefore, it is necessary to modify the surface of culture vessels. This paper aims to characterise the use of two distinct variants of PC12 cells as well as describe their differentiation and neuronal outgrowth with diverse NGF concentrations (rat or human origin) on various surfaces. In our study, we evaluated cell morphology, neurite length, density and outgrowth (measured spectrofluorimetrically), and expression of neuronal biomarkers (doublecortin and NeuN). We found that the collagen coating was the most versatile method of surface modification for both cell lines. For adherent cells, the coating was definitely less important, and the poly-d-lysine surface was as good as collagen. We also demonstrated that the concentration of NGF is of great importance for the degree of differentiation of cells. For suspension cells, we achieved the best neuronal characteristics (length and density of neurites) after 14 days of incubation with 100 ng/mL NGF (change every 48 h), while for adherent cells after 3–5 days, after which they began to proliferate. In the PC12 cell line, doublecortin (DCX) expression in the cytoplasm and NeuN in the cell nucleus were found. In turn, in the PC12 Adh line, DCX was not expressed, and NeuN expression was located in the entire cell (both in the nucleus and cytoplasm). Only the traditional PC12 line grown in suspension after differentiation with NGF should be used for neurobiological studies, especially until the role of the NeuN protein, whose expression has also been noted in the cytoplasm of adherent cells, is well understood.

show abstract

“…How cannabinoids modulate neurogenesis in physiological, epileptic, and other pathological conditions (in both in vitro and in vivo) is further reviewed in References [ 43 , 243 , 251 , 252 ].…”

Section: Cannabinoids and The Endocannabinoid Systemmentioning

confidence: 99%

Neural Stem Cells and Cannabinoids in the Spotlight as Potential Therapy for Epilepsy

Lourenço

Ribeiro-Rodrigues

Sebastião

et al. 2020

IJMS

View full text Add to dashboard Cite

Epilepsy is one of the most common brain diseases worldwide, having a huge burden in society. The main hallmark of epilepsy is the occurrence of spontaneous recurrent seizures, having a tremendous impact on the lives of the patients and of their relatives. Currently, the therapeutic strategies are mostly based on the use of antiepileptic drugs, and because several types of epilepsies are of unknown origin, a high percentage of patients are resistant to the available pharmacotherapy, continuing to experience seizures overtime. Therefore, the search for new drugs and therapeutic targets is highly important. One key aspect to be targeted is the aberrant adult hippocampal neurogenesis (AHN) derived from Neural Stem Cells (NSCs). Indeed, targeting seizure-induced AHN may reduce recurrent seizures and shed some light on the mechanisms of disease. The endocannabinoid system is a known modulator of AHN, and due to the known endogenous antiepileptic properties, it is an interesting candidate for the generation of new antiepileptic drugs. However, further studies and clinical trials are required to investigate the putative mechanisms by which cannabinoids can be used to treat epilepsy. In this manuscript, we will review how cannabinoid-induced modulation of NSCs may promote neural plasticity and whether these drugs can be used as putative antiepileptic treatment.

show abstract

Peripheral Neuron Survival and Outgrowth on Graphene

Cited by 150 publications

References 57 publications

<p>Eating and Mealtime Behaviors in Patients with Autism Spectrum Disorder: Current Perspectives</p>

<p>Eating and Mealtime Behaviors in Patients with Autism Spectrum Disorder: Current Perspectives</p>

PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions

Neural Stem Cells and Cannabinoids in the Spotlight as Potential Therapy for Epilepsy

Contact Info

Product

Resources

About