Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice

Abstract

The aim of the present study is to investigate the role and potential mechanisms of peripheral serotonin in postoperative intra-abdominal adhesion formation in mice. The caecum-rubbing operations were conducted for intra-abdominal adhesion formation modelling in wild-type and Tph1−/− mice. The deficiency of serotonin significantly decreased the adhesion scores, weight loss, and adhesion thickness as well as levels of collagen fibres and hydroxyproline in the adhesive tissues. The Tph1−/− mice exhibited a milder inflammatory response and oxidative stress in the adhesive tissues than did the wild-type mice. Moreover, the deficiency of serotonin reduced the levels of PAI-1 and fibrinogen, and raised the t-PA and t-PA/PAI levels in the peritoneal fluids. Moreover, the expressions of CD34, VEGF, TGF-β and 5-HT2B receptor in the adhesive tissues were significantly decreased in the Tph1−/− group mice. Furthermore, the Tph1−/− +5-HTP group showed more severe adhesions than did the Tph1−/− group mice, and the p-chlorophenylalanine (PCPA) could markedly alleviated the adhesion formation in the WT mice. In conclusion, the present study showed that peripheral serotonin regulated postoperative intra-abdominal adhesion formation by facilitating inflammation, oxidative stress, disorder of the fibrinolytic system, angiopoiesis and TGF-β1 expression via the 5-HT2B receptor in the adhesive tissues.

Application of Traditional Chinese Medicines in Postoperative Abdominal Adhesion

“…On the other hand, fibrin deposition contributes to the injury healing to a certain degree. However, disorders of the fibrinolytic process, such as the imbalance of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1), could result in the formation of abdominal adhesion [7]. Collagen deposition is similar to that of fibrin.…”

Intraabdominālu saaugumu veidošanās un norises kompleksie morfopatoģenētiskie aspekti bērniem līdz gada vecumam. Promocijas darbs

“…Nav zināmas proporcijas starp saaugumu veidiem, taču kā biežākais veids tiek aprakstīti postoperatīvie saaugumi (Liakakos et al, 2001). Postoperatīvu intraabdominālu saaugumu veidošanās ir visbiežākā komplikācija pēc intraabdominālām operācijām (Bi et al, 2017).…”

“…Šī matrice ietver leikocītus, eritrocītus, trombocītus, endoteliocītus, epiteliocītus, tuklās šūnas un šūnu atliekas. Iekaisumam ir būtiska nozīme postoperatīvo intraabdominālo saaugumu patoģenēzē(Bi et al, 2017). Piemēram, pirmā morfoloģiskā reakcija pēc seroza bojājuma ir bieza fibrīna slāņa veidošanās, kas tālāko 12 stundu laikā tiek infiltrēts ar neitrofilajiem leikocītiem(Brochhausen et al, 2012).…”

The Complex Morphopathogenetic Aspects of Intraabdominal Adhesions Development and Course in Infants. Summary of the Doctoral Thesis

“…Intensive vascular endothelial growth factor (VEGF) expression is characteristic in adhesions (Bi et al, 2017), which regulates angiogenesis and oxygen delivery to damaged tissue. Nerve fibers are identified in peritoneal adhesions, but general neuroendocrine system markers have not been identified yet.…”

“…Marked neoangiogenesis was detected and it was considered that VEGF might be indirectly involved in the vasculature changes. This is supported by the fact that VEGF is the main mediator in angiogenesis, regulating vessel remodelation in case of tissue injury and inflammation(Wu et al, 2010).There is data on VEGF increase in adhesion being the central factor in the development of adhesions(Bi et al, 2017). The increase of VEGF is characterised as a compensatory mechanism, regulating angiogenesis and promoting nutrients and oxygen to injured tissues(Rout et al, 2000).…”