Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

Câmara, Paulo Eduardo Aguiar Saraiva; Souza, Láuren Machado Drumond de; Pinto, Otávio Henrique Bezerra; Convey, Peter; Amorim, Eduardo Toledo de; Carvalho‐Silva, Micheline; Rosa, Luiz Henrique

doi:10.1017/s0954102021000316

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA was used as a DNA barcode for molecular species identification [ 27 , 33 , 34 , 35 ]. PCR amplicons generated using the universal primers ITS3 and ITS4 [ 36 ] were sequenced commercially by Macrogen Inc. (Seoul, Republic of Korea) using high-throughput paired-end sequencing (2 × 300 bp) on a MiSeq System (Illumina, San Diego, CA, USA), using the MiSeq Reagent Kit v3 (600 cycles).…”

Section: Methodsmentioning

confidence: 99%

Endolithic Fungal Diversity in Antarctic Oligocene Rock Samples Explored Using DNA Metabarcoding

Rabelo,

Gonçalves,

Carvalho

et al. 2024

Biology

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In this study, we evaluated the fungal diversity present associated with cores of Oligocene rocks using a DNA metabarcoding approach. We detected 940,969 DNA reads grouped into 198 amplicon sequence variants (ASVs) representing the phyla Ascomycota, Basidiomycota, Mortierellomycota, Chytridiomycota, Mucoromycota, Rozellomycota, Blastocladiomycota, Monoblepharomycota, Zoopagomycota, Aphelidiomycota (Fungi) and the fungal-like Oomycota (Stramenopila), in rank abundance order. Pseudogymnoascus pannorum, Penicillium sp., Aspergillus sp., Cladosporium sp., Aspergillaceae sp. and Diaporthaceae sp. were assessed to be dominant taxa, with 22 fungal ASVs displaying intermediate abundance and 170 being minor components of the assigned fungal diversity. The data obtained displayed high diversity indices, while rarefaction indicated that the majority of the diversity was detected. However, the diversity indices varied between the cores analysed. The endolithic fungal community detected using a metabarcoding approach in the Oligocene rock samples examined contains a rich and complex mycobiome comprising taxa with different lifestyles, comparable with the diversity reported in recent studies of a range of Antarctic habitats. Due to the high fungal diversity detected, our results suggest the necessity of further research to develop strategies to isolate these fungi in culture for evolutionary, physiological, and biogeochemical studies, and to assess their potential role in biotechnological applications.

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Section: Methodsmentioning

confidence: 99%

Endolithic Fungal Diversity in Antarctic Oligocene Rock Samples Explored Using DNA Metabarcoding

Rabelo,

Gonçalves,

Carvalho

et al. 2024

Biology

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“…The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA was used as a DNA barcode for molecular species identification [13][14][15][16]. PCR amplicons generated using the universal primers ITS3 and ITS4 [17] were sequenced commercially by Macrogen Inc. (South Korea) using high-throughput paired-end sequencing (2 × 300 bp) on a MiSeq System (Illumina), using the MiSeq Reagent Kit v3 (600 cycles) and following the manufacturer's protocol.…”

Section: Dna Extraction Library Construction and Sequencingmentioning

confidence: 99%

Fungal and fungal‐like diversity present in ornithogenically influenced maritime Antarctic soils assessed using metabarcoding

Gonçalves,

Pimenta,

Lopes

et al. 2024

J Basic Microbiol

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We assessed soil fungal and fungal‐like diversity using metabarcoding in ornithogenically influenced soils around nests of the bird species Phalacrocorax atriceps, Macronectes giganteus, Pygoscelis antarcticus, and Pygoscelis adelie on the South Shetland Islands, maritime Antarctic. A total of 1,392,784 fungal DNA reads was obtained and assigned to 186 amplicon sequence variants (ASVs). The dominant fungal phylum was Ascomycota, followed by Basidiomycota, Chytridiomycota, Blastocladiomycota, Rozellomycota, Mortierellomycota, Monoblepharomycota, Aphelidiomycota, Basidiobolomycota, Mucoromycota, and the fungal‐like Oomycota (Stramenopila), in rank order. Antarctomyces sp., Blastocladiomycota sp., Pseudogymnoascus pannorum, Microascaceae sp., Mortierella sp., Lobulomycetales sp., Sordariomycetes sp., Fungal sp., Rhizophydiales sp., Pseudeurotiaceae sp., Chytridiomycota sp. 1, Filobasidiella sp., Tausonia pullulans, Betamyces sp., and Leucosporidium sp. were the most abundant assigned taxa. The fungal assemblages present in the different ornithogenically influenced soils displayed different diversity indices. However, in general, we detected high fungal diversity and few taxa shared between the samples. Despite the polyextreme environmental conditions experienced in these Antarctic soils, the metabarcoding approach detected a rich and complex fungal community dominated by saprophytes, but with some pathogenic taxa also present. The community was dominated by psychrophilic and psychrotolerant taxa, some apparently endemic to Antarctica, and those identified only at higher taxonomic levels, which may represent currently undescribed fungi. The mycobiome detected included taxa characterized by different ecological roles, including saprotrophic, human‐ and animal‐associated, phytopathogenic, mutualistic, and cosmopolitan. These fungi may potentially be dispersed by birds or in the air column over great distances, including between different regions within Antarctica and from South America, Africa, and Oceania.

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“…The main routes proposed for such dispersal include (i) the American pathway, with dispersal via the Neotropics and Patagonia to sub-Antarctic South Georgia and maritime Antarctica, (ii) the African pathway via the East and Central African Mountains to sub-Antarctic islands such as Marion Island and the Kerguelen archipelago, and (iii) the Indomalayan-Malesian pathway from south-east Asia to southeast Australia, New Zealand, and associated islands (Ochyra et al, 2008). Molecular data have recently supported the bipolarity of several moss taxa (Biersma et al 2017;overview in Câmara, Carvalho-Silva, Pinto, et al, 2021;Câmara, Convey, Rangel, et al, 2021;Câmara, De Souza, Pinto, et al, 2021). Muñoz et al (2004) showed the importance of air currents as an LDD mechanism in the Southern Hemisphere for non-flowering plant species distributions, demonstrating a pattern of diaspore transfer from South America towards Africa at mid latitudes.…”

Section: Introductionmentioning

confidence: 97%

Investigating aerial diversity of non‐fungal eukaryotes across a 40° latitudinal transect using DNA metabarcoding

et al. 2023

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The high number of bipolar and widespread bryophyte and microbial taxa in the Antarctic flora suggests the effective Long‐Distance Dispersal (LDD) of spores and other propagules from lower latitudes and even the Northern Hemisphere to Antarctica and the sub‐Antarctic regions. However, few studies have attempted to document the transfer mechanisms by which potential newcomers may arrive in Antarctica. Commonly suggested or assumed mechanisms include transport in air currents, adventitious transfer with migrating or vagrant birds, and with human assistance. In this study, we investigated the biodiversity present in the air along a 40° latitudinal transect, from the port city of Rio de Janeiro, Brazil (ca. 22°S) to Comandante Ferraz Station on King George Island (South Shetland Islands, ca. 62°S), maritime Antarctica, to shed light on the potential role of LDD in species distribution. Air samples were collected in October 2021 on the Brazilian polar support vessel Ary Rongel using air filters with membranes of 0.22 μm. Total DNA was extracted from the filters and the Internal Transcribed Spacer (ITS2) DNA sequence was used for metabarcoding. A total of 53 non‐fungal taxa representing three kingdoms (most abundantly Viridiplantae) and six phyla (most abundantly Bryophyta and Magnoliophyta) were assigned from the sequences found. Aerial biodiversity was greater closer to the coast and generally decreased with increasing latitude, although a small increase was apparent in the South Shetland Islands. The taxa assigned are generally present in coastal biomes, although a small proportion of the assignments represented taxa of more distant origin, supporting the occurrence of LDD in the air column.

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Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

Cited by 6 publications

References 43 publications

Endolithic Fungal Diversity in Antarctic Oligocene Rock Samples Explored Using DNA Metabarcoding

Endolithic Fungal Diversity in Antarctic Oligocene Rock Samples Explored Using DNA Metabarcoding

Fungal and fungal‐like diversity present in ornithogenically influenced maritime Antarctic soils assessed using metabarcoding

Investigating aerial diversity of non‐fungal eukaryotes across a 40° latitudinal transect using DNA metabarcoding

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