Periplasmic binding protein-based magnetic isolation and detection of thiamine in complex biological matrices

Edwards, Katie A.; Randall, Eileen A.; Tu‐Maung, Nicole; Sannino, David R.; Feder, Seth; Angert, Esther R.; Kraft, Clifford E.

doi:10.1016/j.talanta.2019.120168

Cited by 9 publications

(8 citation statements)

References 28 publications

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“…The linear regression equation was F = 19.247 + 2.452 × 10 8 × C thiamine with a correlation coefficient of 0.9984. There was a good linear correlation between the fluorescence intensity and the thiamine concentration in the range of 5 × 10 −8 to 5 × 10 −5 M. The lower limit of detection of Hb-Cu 3 (PO 4 ) 2 NFs for thiamine was found to be 4.8 × 10 −8 M, which is similar to the HRP-based fluorescent method for the detection of thiamine [36]. It is almost believable that its ability to detect thiamine is similar to the HRP-based fluorescent method.…”

Section: Calibration Curve For Thiamine Detectionsupporting

confidence: 56%

Fluorometric Detection of Thiamine Based on Hemoglobin–Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity

Zou

Zhang

et al. 2020

Sensors

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Component analysis plays an important role in food production, pharmaceutics and agriculture. Nanozymes have attracted wide attention in analytical applications for their enzyme-like properties. In this work, a fluorometric method is described for the determination of thiamine (TH) (vitamin B1) based on hemoglobin–Cu3(PO4)2 nanoflowers (Hb–Cu3(PO4)2 NFs) with peroxidase-like properties. The Hb–Cu3(PO4)2 NFs catalyzed the decomposition of H2O2 into ·OH radicals in an alkaline solution that could efficiently react with nonfluorescent thiamine to fluoresce thiochrome. The fluorescence of thiochrome was further enhanced with a nonionic surfactant, Tween 80. Under optimal reaction conditions, the linear range for thiamine was from 5 × 10−8 to 5 × 10−5 mol/L. The correlation coefficient for the calibration curve and the limit of detection (LOD) were 0.9972 and 4.8 × 10−8 mol/L, respectively. The other vitamins did not bring about any obvious changes in fluorescence. The developed method based on hybrid nanoflowers is specific, pragmatically simple and sensitive, and has potential for application in thiamine detection.

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Section: Calibration Curve For Thiamine Detectionsupporting

confidence: 56%

Fluorometric Detection of Thiamine Based on Hemoglobin–Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity

Zou

Zhang

et al. 2020

Sensors

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“…SBPs have additional applications as biosensors, 20 and when immobilized, as stringent capture reagents for their specific ligands. 21 Creation of a solid-supported ligand for capturing the cognate SBP involves identifying an appropriate site on the ligand for covalent modification and assessing the tolerance of the SBP for an extended linker. We previously showed that SmoF could bind SQ and various anomeric derivatives, with a constellation of interactions between protein and ligand at the sulfonate group and other non-anomeric sites that are presumably required for SQ specificity.…”

Section: Discussionmentioning

confidence: 99%

“…SBPs have additional applications as biosensors, 20 and when immobilized, as stringent capture reagents for their specific ligands. 21…”

Section: Discussionmentioning

confidence: 99%

Capture-and-release of a sulfoquinovose-binding protein on sulfoquinovose-modified agarose

Arumapperuma,

Snow,

Lee

et al. 2024

Org. Biomol. Chem.

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“…The pET-28 plasmid containing the gene for C. botulinum thiaminase I (BcmE) 50 was kindly provided to us by Dr. Chunhao Li and transformed into E. coli BL21. This His-tagged thiaminase was overexpressed in E. coli and was purified using a Ni-chelate resin using previously reported procedures 51 . The concentration of the purified enzyme was determined using the microBCA assay (ThermoFisher) and purity assessed using SDS-PAGE with Coomassie staining (see Supplementary Fig.…”

Section: Methodsmentioning

confidence: 99%

Dietary factors potentially impacting thiaminase I-mediated thiamine deficiency

Edwards

Randall

Wolfe

et al. 2023

Sci Rep

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Fish population declines from thiamine (vitamin B1) deficiency have been widespread in ecologically and economically valuable organisms, ranging from the Great Lakes to the Baltic Sea and, most recently, the California coast. Thiamine deficiencies in predatory fishes are often attributed to a diet of prey fishes with high levels of thiamine-degrading (e.g., thiaminase) enzymes, such as alewives, rainbow smelt, and anchovies. Since their discovery, thiaminase I enzymes have been recognized for breaking down thiamine into its pyrimidine and thiazole moieties using various nucleophilic co-substrates to afford cleavage, but these studies have not thoroughly considered other factors that could modify enzyme activity. We found the thiaminase I enzyme from Clostridium botulinum efficiently degrades thiamine in the presence of pyridoxine (vitamin B6) as a co-substrate but has relatively limited activity in the presence of nicotinic acid (vitamin B3). Using fluorescence measurements, thiamine degradation in an over-the-counter complete multivitamin formulation was inhibited, and a B-complex formulation required co-substrate supplementation for maximal thiamine depletion. These studies prompted the evaluation of specific constituents contributing to thiaminase I inhibition by both chromatography and fluorescence assays: Cu2+ potently and irreversibly inhibited thiamine degradation; ascorbic acid was a strong but reversible inhibitor; Fe2+, Mn2+ and Fe3+ modulated thiamine degradation to a lesser degree. The enhancement by pyridoxine and inhibition by Cu2+ extended to thiaminase-mediated degradation from Burkholderia thailandensis, Paenibacillus thiaminolyticus, and Paenibacillus apiarius in tryptic soy broth supernatants. These co-substrate limitations and the common presence of inhibitory dietary factors complement recent studies reporting that the intended function of thiaminase enzymes is to recycle thiamine breakdown products for thiamine synthesis, not thiamine degradation.

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Periplasmic binding protein-based magnetic isolation and detection of thiamine in complex biological matrices

Cited by 9 publications

References 28 publications

Fluorometric Detection of Thiamine Based on Hemoglobin–Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity

Fluorometric Detection of Thiamine Based on Hemoglobin–Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity

Capture-and-release of a sulfoquinovose-binding protein on sulfoquinovose-modified agarose

Dietary factors potentially impacting thiaminase I-mediated thiamine deficiency

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