Periplocin Inhibited Gliomas Migration by Targeting Migrasome Related Gene TSPAN6

Abstract

Glioma is a life-threatening brain tumor characterized by rapid growth and resistance to treatment. Periplocin, the monomeric compound, exhibits anti-tumor activities. However, the action of periplocin in gliomas remain unclear. Migrasome, a vesicle-like structure derived from the tips or intersections of contractile filaments in migrating cells, was investigated in this study to explore the antitumor activity of periplocin in glioma and elucidate its mechanism. The proliferation, apoptosis, migration and MMP in periplocin-treated LN229 and KNS-89 cells were examined. In order to revealed the mechanism of Periplocin inhibition of migration. Additionally, we revealed the relation of Migrasome-related genes with glioma based on TCGA database analysis, and we found TSPAN6 may be the direct target in glioma, Periplocin could inhibited LN229 and KNS-89 cell proliferation and migration, and promoted their apoptosis. Moreover, targeting TSPAN6 resulted in reduced cell viability and migration, as well as increased apoptosis in glioma cells.