Perisinusoidal fat-storing cells are the main vitamin A storage sites in rat liver

The niche of stellate cells within rat liver #

“…1,27,28 The rats were obtained from the local animal facility of the university, and all animals received care according to the German Animal Welfare Act. Cell culture techniques and the procedures of semiquantitative and quantitative reverse transcription polymerase chain reaction (RT-PCR) as well as western blot and immunofluorescence staining techniques were carried out as described.…”

“…Hepatic stellate cells (HSCs) display characteristic lipid droplets that contain vitamin A mainly as retinyl palmitate. 1 After liver injury, quiescent HSCs become activated, lose their vitamin A stores, and develop into contractile myofibroblast-like cells, which secrete extracellular matrix proteins. It is widely accepted that these HSC-derived myofibroblast-like cells contribute to liver fibrosis.…”

Relaxin receptors in hepatic stellate cells and cirrhotic liver

“…Rat HSC were isolated from SAS-Sprague-Dawley rats (400-500 g) using sequential perfusion with pronase and collagenase, followed by density gradient centrifugation in Nycodenz as described previously [19]. Cell viability was monitored by trypan blue exclusion, and HSC identified by their typical morphology and the presence of fat droplets under UV illumination.…”

Cell cycle protein profile of the hepatic stellate cells (HSCs) in dimethylnitrosamine-induced rat hepatic fibrosis

“…This was loaded onto a 17.2% Nycodenz solution and covered the layer with 1 ml of HBSS. Following centrifugation for 15 min at 3,000 g, the cells at the top of the Nycodenz were collected, and washed by centrifugation (Hendriks et al, 1985;Vyas et al, 1995;Geerts et al, 1998).…”