Periurethral Bulking Agent Injection in the Treatment of Female Stress Urinary Incontinence

Lightner, Deborah J.; Knoedler, John J.; Linder, Brian J.

doi:10.1007/978-3-319-49855-3_27

Cited by 2 publications

(1 citation statement)

References 67 publications

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“…However, current UI therapies have limitations, including repeated procedures, short-lived therapeutic effects, and a lack of effect on the restoration of bladder or urinary SMCs. The most frequent complications from bulking-agent-injection therapy are dysuria, hematuria, or UI due to other causes [ 27 ]. Therefore, the development of alternative therapeutic strategies is required.…”

Section: Discussionmentioning

confidence: 99%

Combination of PD98059 and TGF-β1 Efficiently Differentiates Human Urine-Derived Stem Cells into Smooth Muscle Cells

Hwang

Cha

Kim

et al. 2021

IJMS

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Pluripotent adult stem cells have potential applications in cell therapy and tissue engineering. Urine-derived stem cells (UDSCs) differentiate into various cell types. Here, we attempted to differentiate human UDSCs (hUDSCs) into smooth muscle cells (SMCs) using transforming growth factor-beta 1 (TGF-β1) and/or PD98059, an extracellular signal-regulated kinase (ERK) inhibitor. Both quantitative polymerase chain reaction (qPCR) and Western blot analysis showed that the expression of messenger ribonucleic acid (mRNA) and proteins for alpha-smooth muscle actin (α-SMA), calponin (CNN1), and smooth muscle myosin heavy chain (SM-MHC), which are specific markers for SMCs, increased on day 9 after differentiation and again on day 14. The differentiated cells from human UDSCs (hUDSCs) with a combination of TGF-β1 and PD98059 showed the highest expression of SMC marker proteins. Immunocytochemical staining performed to assess the molecular expression revealed CNN and α-SMA colocalizing in the cytoplasm. The cells that differentiated from hUDSCs with a combination of TGF-β1 and PD98059 showed the strongest expression for CNN1, α-SMA, and SM-MHC. Functional testing of the differentiated cells revealed a stronger contractile capacity for the cells differentiated with a combination of PD98059 and TGF-β1 than those differentiated with a single factor. These results suggest the combination of PD98059 and TGF-β1 to be a more effective differentiation method and that differentiated SMCs could be used for restoring the functions of the sphincter muscle or bladder.

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Section: Discussionmentioning

confidence: 99%