Permanent cannulation of the hepatic portal vein in rats

Sablé-Amplis, R.; Abadie, Delphine

doi:10.1152/jappl.1975.38.2.358

Cited by 16 publications

(3 citation statements)

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“…The subsequent decline in blood glucose after a hyperglycemic period observed in the chronically catheterized rats also has been reported in rats (16) and dogs (21). This decline in the blood glucose concentrations probably is secondary to an increase in insulin concentrations after a hyperglycemic period (1 6, 21, 22).…”

Section: Discussionsupporting

confidence: 54%

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Portal Venous and Aortic Glucose and Lactate Changes in a Chronically Catheterized Rat

1988

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ABSTRACI'. A method for portal venous, aortic, and gastric cannulation in the rat is described. Changes in mean portal venous and aortic blood glucose and lactate concentrations after an intragastric infusion of d-glucose to chronically catheterized rats (after regaining preoperative weight) were compared to those of acutely catheterized rats (1 h after catheter placement). Both portal venous and aortic catheters were patent for blood sampling in 67% of rats 8 days after catheter placement and 42% after 14 days. After intragastric infusion of 10 ml of 0.29 M dextrose, mean aortic blood glucose and lactate concentrations in chronically catheterized rats increased 80 and 73%, respectively, 15 min after infusion and decreased to preinfusion concentrations by 60 min. In acutely catheterized rats, mean aortic glucose and lactate concentrations increased linearly for 60 min. The concentration differences between portal venous and aortic blood glucose and lactate concentrations in chronically catheterized rats were not significantly different than baseline differences. The portal venous glucose concentrations were significantly greater than aortic concentrations in acutely catheterized rats 15, 30, and 45 min after the glucose infusion when compared to baseline differences. These data suggest that the rate of glucose uptake and metabolism to lactate is significantly altered in acutely catheterized rats when compared to chronically catheterized animals. (Pediatr Res 23: 235-240,1988) Abbreviation AVC, area under the curve.(4); manual manipulation of the bowel can alter mesenteric blood flow and anesthesia can alter mesenteric blood flow and intestinal perfusion (5). These factors can alter intestinal perfusion and oxygen delivery. In contrast, studies that use the technique of chronic catheterization avoid these artifacts (6).We have developed a rat model in which the portal vein, aorta, and stomach are catheterized chronically. Using this preparation, we serially determined in vivo portal venous and aortic blood concentrations of glucose, lactate, and alanine after a bolus intragastric infusion of glucose in nonstressed animals. We also determined the effects of surgery and anesthesia on portal venous and aortic blood glucose, lactate, and alanine concentrations. MATERIALS AND METHODS Animals.All experiments were performed in vivo using adult male albino Sprague-Dawley rats (200-300 g body weight). These animals were obtained from Simonsen Labs (Gilroy, CA) and allowed free access to water and rat food.Catheters. The portal vein catheter consisted of two 1.5 cm segments of PE 60 tubing (Clay Adams, Parsippany, NJ; ID 0.76 mm, OD 1.22 mm) connected to the ends of a 4.5-cm silastic tube (Dow Corning, Midland, MI; silastic ID 0.64 mm, OD 1.19 mm). The silastic tubing was stretched securely over the segment of PE 60 tubing. The ends of the PE 60 tubing were cut to a sharp point at an angle of approximately 45". The catheter was then placed over an 8.89-cm 25-gauge spinal needle (Becton Dickinson, Rutherford, NJ) which s...

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Section: Discussionsupporting

confidence: 54%

“…Previous techniques of portal venous catheterization have included ligation of fourth generation mesenteric veins (14) or interruption of the portal vein with placement of a T connector into the portal vein (15,16). All other methods for cannulation of the portal vein involve cutting the portal vein and inserting a catheter (17).…”

Section: Discussionmentioning

confidence: 99%