Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2011–30 September 2011

Abstract

This article documents the addition of 299 microsatellite marker loci and nine pairs of single-nucleotide polymorphism (SNP) EPIC primers to the Molecular Ecology Resources (MER) Database. Loci were developed for the following species: Alosa pseudoharengus, Alosa aestivalis, Aphis spiraecola, Argopecten purpuratus, Coreoleuciscus splendidus, Garra gotyla, Hippodamia convergens, Linnaea borealis, Menippe mercenaria, Menippe adina, Parus major, Pinus densiflora, Portunus trituberculatus, Procontarinia mangiferae, Rhynchophorus ferrugineus, Schizothorax richardsonii, Scophthalmus rhombus, Tetraponera aethiops, Thaumetopoea pityocampa, Tuta absoluta and Ugni molinae. These loci were cross-tested on the following species: Barilius bendelisis, Chiromantes haematocheir, Eriocheir sinensis, Eucalyptus camaldulensis, Eucalyptus cladocalix, Eucalyptus globulus, Garra litaninsis vishwanath, Garra para lissorhynchus, Guindilla trinervis, Hemigrapsus sanguineus, Luma chequen. Guayaba, Myrceugenia colchagüensis, Myrceugenia correifolia, Myrceugenia exsucca, Parasesarma plicatum, Parus major, Portunus pelagicus, Psidium guayaba, Schizothorax richardsonii, Scophthalmus maximus, Tetraponera latifrons, Thaumetopoea bonjeani, Thaumetopoea ispartensis, Thaumetopoea libanotica, Thaumetopoea pinivora, Thaumetopoea pityocampa ena clade, Thaumetopoea solitaria, Thaumetopoea wilkinsoni and Tor putitora. This article also documents the addition of nine EPIC primer pairs for Euphaea decorata, Euphaea formosa, Euphaea ornata and Euphaea yayeyamana.

Reconciling seascape genetics and fisheries science in three codistributed flatfishes

“…(2000) ( Sma5‐111INRA and SmA1‐152INRA ), two EST‐derived markers described in Bouza et al. (2008) ( SmaUSC‐E32 and SmaUSC‐E41 ) and 10 novel markers developed through gDNA pyrosequencing (GS FLX Titanium, Roche; ScoR26 , ScoR28 , ScoR12 , ScoR27 , ScoR16 , ScoR5 , ScoR2 , ScoR11 , ScoR4 and ScoR6 ) (Molecular Ecology Resources Primer Development et al., 2012). Microsatellite markers were combined in three multiplex reactions, each consisting of an initial denaturation step of 7 min at 95°C, followed by 30 cycles of 30 s at 95°C, 90 s at 54°C (multiplex 2 and 3) or 56°C (multiplex 1) and 60 s at 72°C after a final elongation of 30 s at 60°C, and cooled down to 10°C.…”

Human disturbance causes the formation of a hybrid swarm between two naturally sympatric fish species

“…We examined the variation at 21 polymorphic microsatellites: 15 loci developed for alewife ( Ap010, Ap033, Ap037, Ap038, Ap047, Ap058, Ap070, Ap071 ) and blueback herring ( Aa046, Aa070, Aa074, Aa081, Aa082, Aa091, Aa093 ) (A'Hara et al . ) that were common across studies and six additional loci developed for alewife ( Aps1, Aps2A ; Bentzen & Paterson ), blueback herring ( Aa039; A'Hara et al . ) and American shad ( A. sapidissima ) [ AsaD042, AsaC249 (Julian & Bartron ), Asa8 (Waters et al .…”

“…) that were common across studies and six additional loci developed for alewife ( Aps1, Aps2A ; Bentzen & Paterson ), blueback herring ( Aa039; A'Hara et al . ) and American shad ( A. sapidissima ) [ AsaD042, AsaC249 (Julian & Bartron ), Asa8 (Waters et al . )] that were examined by McBride () alone.…”

Genetic stock composition of marine bycatch reveals disproportional impacts on depleted river herring genetic stocks

“…Amplification, size-fragment analysis, and scoring were conducted following (A'Hara et al 2012). To confirm consistency in scoring and reproducibility of genotypes, positive and negative controls were used.…”