Permeable tubes increase the length of the gap that regenerating axons can span

Jenq, Chung Bii; Coggeshall, Richard E.

doi:10.1016/0006-8993(87)90379-9

Cited by 95 publications

(54 citation statements)

References 12 publications

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“…Some reports demonstrate benefits of using porous nerve conduits, which are hypothesized to facilitate the flow of nutrients and factors that enhances cell migration and fibrin cable formation, 74,75 while others show the benefits of using nonporous conduits, which better contain the fluid and cells secreted by the nerve stumps at the injury site, in direct association with the regenerating axons. 76 However, very few studies directly compare porous to nonporous nerve conduits.…”

mentioning

confidence: 99%

Enhanced Femoral Nerve Regeneration After Tubulization with a Tyrosine-Derived Polycarbonate Terpolymer: Effects of Protein Adsorption and Independence of Conduit Porosity

Ezra

Bushman

Shreiber

et al. 2013

Tissue Engineering Part A

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Following complete nerve transection, entubulation of the nerve stumps helps guide axons to reconnect distally. In this study, a biodegradable and noncytotoxic tyrosine-derived polycarbonate terpolymer composed of 89.5 mol% desaminotyrosyl tyrosine ethyl ester (DTE), 10 mol% desaminotyrosyl tyrosine (DT), and 0.5 mol% poly(ethylene glycol) (PEG, molecular weight [Mw] = 1 kDa) [designated as E10-0.5(1K)] was used to fabricate conduits for peripheral nerve regeneration. These conduits were evaluated against commercially available nonporous polyethylene (PE) tubes. The two materials are characterized in vitro for differences in surface properties, and the conduits are then evaluated in vivo in a critical-sized nerve defect in the mouse femoral nerve model. Conduits were fabricated from E10-0.5(1K) in both porous [P-E10-0.5(1K)] and nonporous [NP-E10-0.5(1K)] configurations. The results illustrate that adsorption of laminin, fibronectin, and collagen type I was enhanced on E10-0.5(1K) compared to PE. In addition, in vivo the E10-0.5(1K) conduits improved functional recovery over PE conduits, producing regenerated nerves with a fivefold increase in the number of axons, and an eightfold increase in the percentage of myelinated axons. These increases were observed for both P-E10-0.5(1K) and NP-E10-0.5(1K) after 15 weeks. When conduits were removed at 7 or 14 days following implantation, an increase in Schwann cell proteins and fibrin matrix formation was observed in E10-0.5(1K) conduits over PE conduits. These results indicate that E10-0.5(1K) is a pro-regenerative material for peripheral nerves and that the porosity of P-E10-0.5(1K) conduits was inconsequential in this model of nerve injury.

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mentioning

confidence: 99%

Enhanced Femoral Nerve Regeneration After Tubulization with a Tyrosine-Derived Polycarbonate Terpolymer: Effects of Protein Adsorption and Independence of Conduit Porosity

Ezra

Bushman

Shreiber

et al. 2013

Tissue Engineering Part A

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“…Es importante señalar que nuestros hallazgos son contrarios a varios informes sobre otros modelos de tubos impermeables, en los cuales es mayor el número de fibras a nivel distal (18)(19)(20)(21)(22)(23)(24)(25). Los datos existentes en la literatura muestran que la entrada de células a las cámaras permeables incrementa el número de fibras CAMARA PERONEAL mielínicas y estimula la regeneración (6,28,29). pm (p<0,001), que en las cámaras de 10 pm (p<0,05).…”

Section: Regeneracion De Nervio Perlferico En Camaras Permeablesunclassified

Estudio de la regeneración de nervio periférico a través de cámaras permeables de silicona con poros de 10 y 60 micras

Alzate

Sutachán²,

Duque³

et al. 1999

biomedica

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ResumenSe entubó el nervio ciático de ratas, luego de un corte transverso, utilizando una cámara de silicona de 14 mm de largo que tenía dos ventanas de 8 mm de largo cubiertas con malla de 10 o 60 pm. Después de 6 meses, los animales se perfundieron con una mezcla de glutaraldehído y paraformaldehído, y se evaluó el aspecto microscópico de los nervios regenerados en dos niveles dentro de la cámara (3 mm y nivel medio) y 5 mm distal a ésta. Se contó el número de fibras mielínicas presentes en cada nivel y tratamiento, y se realizó una comparación entre ellos. Se encontró una importante desorganización del endoneurio a nivel medio de la cámara. Distalmente, los conteos de fibras mielínicas fueron muy bajos. No se observaron diferencias significativas entre los grupos con mallas de 10 y 60 pm. En conclusión, la presencia de poros de 10 o 60 pm no impide la entrada masiva de fibroblastos a la cámara, llevando a una marcada desorganización del nervio regenerado.Palabras clave: regeneración, nervio periférico, entubación, fibroblasto, permeabilidad.A study of peripheral nerve regeneration in permeable silicone chambers with 10 and 60 micrometer pores Abstract Rat sciatic nerves were transected and intubated with a fourteen millimeter lengthsilicone chamber, with two 8 mm windows covered with either a 10 or 60 pm mesh. After six months, the animals were perfused with a glutaraldehyde and paraformaldehyde mixture and the regenerated nerve histology was evaluated at two levels within the chamber (3 mm and midlevel) and 5 mm distal to the chamber. The myelinated nerve fibers were counted and compared for al1 levels and treatments. We found a disorganized endoneurium at midchamber level. Distally, a very low myelinated nerve fibers count was registered. No significant differences were observed between 10 and 60 vm meshes.We conclude that 10 or 60 pm meshes do not control the massive invasion of the chamber by fibroblasts, leading to a highly disorganized regenerated nerve.

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“…The distance from the tube wall to the center of the site of injury (d1) and the distance of the tube wall itself (d2) are important factors that affect radial permeability. Hollow tubular constructs with permeable outer tube walls perform significantly better than with impermeable outer tube walls 50 . Distinct tubular constructs have been made with either fully-permeable or semipermeable tube walls or luminal fillers, where the latter prevents the entry of myofibroblasts into the site of injury 47 .…”

Section: Biomimetic Design Is a Leading Approach In The Development Omentioning

confidence: 99%

“…Historically, hollow tubular constructs composed of non-permeable silicone were easily surpassed in terms of repair capacity when their outer walls were replaced by permeable silicone walls 50 . To further understand the effects of permeability, in 2005 the role of structural parameters such as wall thickness and porosity were assessed using a tubular construct with both the tube wall and luminal filler composed of collagen 51 .…”

Section: Biomimetic Design Is a Leading Approach In The Development Omentioning

confidence: 99%

Advances in Nerve Guidance Conduit-Based Therapeutics for Peripheral Nerve Repair

Lackington

Ryan

O’Brien

2017

ACS Biomater. Sci. Eng.

103

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Permeable tubes increase the length of the gap that regenerating axons can span

Cited by 95 publications

References 12 publications

Enhanced Femoral Nerve Regeneration After Tubulization with a Tyrosine-Derived Polycarbonate Terpolymer: Effects of Protein Adsorption and Independence of Conduit Porosity

Enhanced Femoral Nerve Regeneration After Tubulization with a Tyrosine-Derived Polycarbonate Terpolymer: Effects of Protein Adsorption and Independence of Conduit Porosity

Estudio de la regeneración de nervio periférico a través de cámaras permeables de silicona con poros de 10 y 60 micras

Advances in Nerve Guidance Conduit-Based Therapeutics for Peripheral Nerve Repair

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