Peroxidase binding to cell-bound Concanavalin A

Huet, Christian

doi:10.1016/0014-4827(74)90816-7

Cited by 14 publications

(1 citation statement)

References 10 publications

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“…The supernatant fluid, separated by centrifugation, was examined for its hemagglutinating activity against papain-treated erythrocytes as compared to the unadsorbed preparation. Test for Peroxidase Binding.-Peroxidase binding to the surface of protozoa by means of the Pseudomonas mannosephilic lectins was examined according to the method of Huet & Bernadac (12). The horseradish peroxidase was removed from the cell surface by mannose, and its activity was determined with o-dianisidine.…”

Section: Methodsmentioning

confidence: 99%

Interactions of Pseudomonas Aeruginosa Hemagglutinins With Euglena Gracilis, Chlamydomonas Reinhardi, and Tetrahymena Pyriformis

Sharabi

Gilboa‐Garber

1980

The Journal of Protozoology

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The galactosephilic and mannosephilic hemagglutinins of Pseudomonas aeruginosa adsorbed onto Euglena gracilis, Chlamydomonas reinhardi, and Tetrahymena pyriformis. Furthermore, peroxidase binding to the 3 protozoan species was shown to be mediated by these lectins. Binding of Pseudomonas lectins to E. gracilis and C. reinhardi caused their specific agglutination, whereas no agglutination was observed with T. pyriformis, even after treatment by papain or by NaF. Added to the culture medium, the Pseudomonas hemagglutins stimulated growth of E. gracilis and T. pyriformis due to their binding to these protozoa; this effect was partly inhibited by the specific sugar.

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Section: Methodsmentioning

confidence: 99%