Peroxidase-modified electrodes: Fundamentals and application

Ruzgas, Tautgirdas; Csöregi, Elisabeth; Emnéus, Jenny; Gorton, Lo; Markó-Varga, György

doi:10.1016/0003-2670(96)00169-9

Cited by 514 publications

(372 citation statements)

References 167 publications

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“…Other research groups reported significant different potentials (E pa ∼ 160 mV and E pc ∼ 190 mV) using GC (which often shows high overpotential to electron transfer) as the base electrode. 61 Our results are closer to half of these values suggesting more efficient ET of HRP modified-GFNs. The DET reaction between cofactor of HRP and the electrode surface was due to the favored orientation of HRP molecule acting as the conducting channels for the electron transfer.…”

Section: Enzyme: Horseradish Peroxidase (Hrp)supporting

confidence: 74%

Probing the nature of electron transfer in metalloproteins on graphene-family materials as nanobiocatalytic scaffold using electrochemistry

Gupta

Irihamye

2015

AIP Advances

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Graphene-based nanomaterials have shown great promise not only in nanoelectronics due to ultrahigh electron mobility but also as biocatalytic scaffolds owing to irreversible protein surface adsorption and facilitating direct electron transfer. In this work, we synthesized stable dispersions of graphene using liquid-phase exfoliation approach based on non-covalent interactions between graphene and 1-pyrenesulfonic acid sodium salt (Py–1SO3), 1-pyrenemethylamine salt (Py − Me-NH2) and Pluronic® P-123 surfactant using only water as solvent compatible with biomolecules. The resulting graphene nanoplatelets (Gr_LPE) are characterized by a combination of analytical (microscopy and spectroscopy) techniques revealing mono- to few-layer graphene displaying that the exfoliation efficiency strongly depends upon the type of pyrene-based salts and organic surfactants. Moreover being completely water-based approach, we build robust nanoscaffolds of graphene-family nanomaterials (GFNs) namely, monolayer graphene, Gr_LPE (the one prepared with Pluronic® P-123), graphene oxide (GO) and its reduced form (rGO) on glassy carbon electrode surface with three important metalloproteins include cytochrome c (Cyt c) [for electron transfer], myoglobin (Mb) [for oxygen storage] and horseradish peroxidase (HRP) [for catalyzing the biochemical reaction]. In order to demonstrate the nanobiocatalytical activity of these proteins, we used electrochemical interfacial direct electron transfer (DET) kinetics and attempt to determine the rate constant (kET) using two different analytical approaches namely, linear sweep voltammetry and Laviron’s theory. We elucidated that all of the metalloproteins retain their structural integrity (secondary structure) upon forming mixtures with GFNs confirmed through optical and vibrational spectroscopy and biological activity using electrochemistry. Among the GFNs studied, Gr-LPE, GO and rGO support the efficient electrical wiring of the redox centers (with an increase in catalytic efficiency of Cyt c and Mb in the presence of GFNs attributed partially to the surface functional (carboxyl, epoxide and hydroxyl) groups on GO and rGO facilitating rapid charge transfer.

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Section: Enzyme: Horseradish Peroxidase (Hrp)supporting

confidence: 74%

Probing the nature of electron transfer in metalloproteins on graphene-family materials as nanobiocatalytic scaffold using electrochemistry

Gupta

Irihamye

2015

AIP Advances

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“…O procedimento é simples e a regeneração da superfície é feita através da renovação da pasta da ponta do biossensor. Excelentes revisões sobre eletrodos de pasta de carbono e eletrodos modificados com peroxidase foram recentemente publicadas [14][15][16][17] . A peroxidase na presença de peróxido de hidrogênio catalisa a oxidação de alguns substratos doadores de prótons como mono e difenóis, polifenóis, aminofenóis entre outros.…”

Section: Introductionunclassified

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Vieira¹,

Lupetti²,

Fatibello‐Filho³

2003

Quím. Nova

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were investigated as the source of peroxidase (POD: EC 1.11.1.7). Among those, zucchini (Cucurbita pepo) crude extract was found to be the best one. This enzyme in the presence of hydrogen peroxide catalyses the oxidation of paracetamol to N-acetylp-benzoquinoneimine which the electrochemical reduction back to paracetamol was obtained at a peak potential of -0.10V. A cyclic voltammetric study was performed by scanning the potential from + 0.5 to -0.5 V. The recovery of paracetamol from two samples ranged from 97.3 to 106% and a rectilinear calibration curve for paracetamol concentration from 1.2x10 -4 to 2.5x10 -3 mol L -1 (r=0.9965) were obtained. The detection limit was 6.9x10 -5 mol L -1 and the relative standard deviation was less than 1.1% for a solution containing 2.5x10 -3 mol L -1 paracetamol and 2.0x10 -3 mol L -1 hydrogen peroxide (n=12). The results obtained for paracetamol in pharmaceutical products using the proposed biosensor and Pharmacopoeial procedures are in agreement at the 95% confidence level.

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“…An HRP-modified spectrographic graphite electrode (HRP-SPGE) was used for electrochemical detection of hydrogen peroxide (Alpeeva et al, 2005;Ruzgas et al, 1996). Working electrodes with an outside diameter of 3.05 mm were prepared from rods of solid spectroscopic graphite type RW001 (Ringsdorff Werke GmbH, Bonn, Germany).…”

Section: Preparation Of H 2 O 2 Sensitive Hrp-modified Graphite Electmentioning

confidence: 99%

Simultaneous use of electrochemistry and chemiluminescence to detect reactive oxygen species produced by human neutrophils

Shleev

Wetterö

Magnusson

et al. 2008

Cell Biology International

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A novel approach for the simultaneous optical and electrochemical detection of biologically produced reactive oxygen species has been developed and applied. The set-up consists of a luminol-dependent chemiluminescence assay combined with two amperometric •-sensitive amperometric response. By contrast, the H 2 O 2 sensitive biosensor, based on an HRP-modified graphite electrode, was able to reflect the bulk concentration of H 2 O 2 , produced by stimulated neutrophils, and would be very useful in modestly equipped biomedical research laboratories. In summary, the system would also be appropriate for assessment of several other metabolites in different cell types, and tissues of varying complexity, with only minor electrode modifications. _____________________________________________________________________________

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Peroxidase-modified electrodes: Fundamentals and application

Cited by 514 publications

References 167 publications

Probing the nature of electron transfer in metalloproteins on graphene-family materials as nanobiocatalytic scaffold using electrochemistry

Probing the nature of electron transfer in metalloproteins on graphene-family materials as nanobiocatalytic scaffold using electrochemistry

Determinação de paracetamol em produtos farmacêuticos usando um biossensor de pasta de carbono modificado com extrato bruto de abobrinha (Cucurbita pepo)

Simultaneous use of electrochemistry and chemiluminescence to detect reactive oxygen species produced by human neutrophils

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