Peroxidases from Phaeophyceae. I: Extraction and Detection of the Peroxidases

Vilter, H.; Glombitza, Karl‐Werner; Gräwe, Alexander

doi:10.1515/botm.1983.26.7.331

Cited by 52 publications

(32 citation statements)

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“…The first example of this class was a VBrPO isolated from the marine alga A. nodosum [94,95]. Single-crystal X-ray diffraction analysis and X-ray absorption spectroscopy (XAS) revealed that the active sites of bromoperoxidase from A. nodosum [12,96] and chloroperoxidase from C. inaequalis [11,97] are very similar, with vanadium in the center of a slightly distorted trigonal bipyramid and a histidine imidazole as the sole protein bound to the metal ion.…”

Section: Application Of Dft Methods To Vanadium Proteins: Vbrpomentioning

confidence: 99%

Application of DFT methods to the study of the coordination environment of the VO2+ ion in V proteins

et al. 2012

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Density functional theory (DFT) methods were used to simulate the environment of vanadium in several V proteins, such as vanadyl-substituted carboxypeptidase (sites A and B), vanadyl-substituted chloroplast F(1)-ATPase (CF(1); site 3), the reduced inactive form of vanadium bromoperoxidase (VBrPO; low- and high-pH sites), and vanadyl-substituted imidazole glycerol phosphate dehydratase (IGPD; sites α, β, and γ). Structural, electron paramagnetic resonance, and electron spin echo envelope modulation parameters were calculated and compared with the experimental values. All the simulations were performed in water within the framework of the polarizable continuum model. The angular dependence of [Formula: see text] and [Formula: see text] on the dihedral angle θ between the V=O and N-C bonds and on the angle φ between the V=O and V-N bonds, where N is the coordinated aromatic nitrogen atom, was also found. From the results it emerges that it is possible to model the active site of a vanadium protein through DFT methods and determine its structure through the comparison between the calculated and experimental spectroscopic parameters. The calculations confirm that the donor sets of sites B and A of vanadyl-substituted carboxypeptidase are [[Formula: see text], H(2)O, H(2)O, H(2)O] and [N(His)(||), N(His)(⊥), [Formula: see text], H(2)O], and that the donor set of site 3 of CF(1)-ATPase is [[Formula: see text], OH(Thr), H(2)O, H(2)O, [Formula: see text]]. For VBrPO, the coordination modes [N(His)(||), N(His)(∠), OH(Ser), H(2)O, H(2)O(ax)] for the low-pH site and [N(His)(||), N(His)(∠), OH(Ser), OH(-), H(2)O(ax)] or [N(His)(||), N(His)(∠), [Formula: see text], H(2)O] for the high-pH site, with an imidazole ring of histidine strongly displaced from the equatorial plane, can be proposed. Finally, for sites α, β, and γ of IGPD, the subsequent deprotonation of one, two, and three imidazole rings of histidine and the participation of a carboxylate group of a glutamate residue ([N(His)(||), [Formula: see text], H(2)O, H(2)O], [N(His)(||), N(His)(||), [Formula: see text], H(2)O], and [N(His)(||), N(His)(||), [Formula: see text], OH(-), [Formula: see text]], respectively) seems to be the most plausible hypothesis.

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Section: Application Of Dft Methods To Vanadium Proteins: Vbrpomentioning

confidence: 99%

Application of DFT methods to the study of the coordination environment of the VO2+ ion in V proteins

et al. 2012

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“…The IPO activity was determined by following the conversion at 350 nm of I − into I − 3 (ε M =26,400 cm −1 M −1 ) in the presence of H 2 O 2 (Vilter et al 1983). Algal samples (∼0.2 gFW) were frozen in liquid nitrogen and ground together with sterilised sand.…”

Section: Haloperoxidase Activity Analysismentioning

confidence: 99%

Effects of hydrogen peroxide on the content of major volatile halogenated compounds in the red alga Asparagopsis taxiformis (Bonnemaisoniaceae)

et al. 2010

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The genus Asparagopsis is a prolific source of halogenated metabolites. Due to its commercial applications, it has been intensively cultivated in southern Portugal. In the present study, we assess if the internal levels of the major halogenated metabolites (bromoform and dibromoacetic acid) in Asparagopsis taxiformis can be increased with hydrogen peroxide (H 2 O 2 ) addition. Previous studies with red algae showed that the production/release of bromoform can be enhanced by exogenously supplying H 2 O 2 . However, no study has assessed if H 2 O 2 supply enhances the content of secondary metabolites within the biomass. This detail is important as the objective of the proposed research is to enhance the content of these valuable metabolites in the produced biomass. Both the activity of the haloperoxidase enzyme and the metabolite content were assessed on shortterm and long-term incubation periods to H 2 O 2 . To determine the susceptibility of A. taxiformis photosynthetic performance to the imposed oxidative stress, the in vivo fluorescence of photosystem II was monitored. A. taxiformis was shown to be physiologically vulnerable to H 2 O 2 , given the observed decrease of the maximum quantum yield of photosynthesis (F v /F m ). Contrary to what was expected, the presence of H 2 O 2 inhibited the activity of the iodoperoxidase enzyme. Nevertheless, the extracted halogenated metabolites were higher over the first hours of exposure to H 2 O 2 , decreasing after 48 h. These results are probably related to the prosthetic group of the halogenated enzyme in A. taxiformis and the long-term oxidative stress damage of H 2 O 2 exposure. Considering the objective of the proposed research, addition of H 2 O 2 to the cultures, prior (3 h) to biomass harvesting, increases the metabolite content.

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“…One is located inside the thallus, particularly around the conceptacles, and the presence of this enzyme shows a seasonal variation (Wever, 1988;Vilter et al, 1983); maxmal activity is found in winter and spring, when fruiting bodies are present, and a rapid decrease in activity is observed in the summer. Another bromoperoxidase is located at the thallus surface and can be considered to be extracellular (Krenn et aL, 1989b).…”

Section: Tv Amsterdam the Netherlandsmentioning

confidence: 99%

Effects of solar ultraviolet radiation on biogeochemical dynamics in aquatic environments : report of a workshop, Marine Biological Laboratory, Woods Hole, Massachusetts, October 23-26, 1989

Blough¹,

Zepp²

1990

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Peroxidases from Phaeophyceae. I: Extraction and Detection of the Peroxidases

Cited by 52 publications

References 0 publications

Application of DFT methods to the study of the coordination environment of the VO2+ ion in V proteins

Application of DFT methods to the study of the coordination environment of the VO2+ ion in V proteins

Effects of hydrogen peroxide on the content of major volatile halogenated compounds in the red alga Asparagopsis taxiformis (Bonnemaisoniaceae)

Effects of solar ultraviolet radiation on biogeochemical dynamics in aquatic environments : report of a workshop, Marine Biological Laboratory, Woods Hole, Massachusetts, October 23-26, 1989

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