Peroxidases, Iactoferrin and lysozyme in peripheral blood neutrophils, gingival crevicular fluid and whole saliva of patients with localized juvenile periodontitis

Suomalainen, Kimmo; Saxén, Lauri; Vilja, Pekka; Tenovuo, Jorma

doi:10.1111/j.1601-0825.1996.tb00213.x

Cited by 25 publications

(19 citation statements)

References 30 publications

(20 reference statements)

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“…The higher expression of this glycoprotein in individuals with aggressive periodontitis is in agreement with a previous report 10 , but is in contrast to others 14,32 . The differences in these two studies from the present could be at least in part explained by variations in samples that were analyzed (i.e.…”

Section: Discussionsupporting

confidence: 92%

“…Among other samples, paraffin-stimulated whole saliva was obtained from these individuals and from periodontally healthy controls. Differences between groups and with respect to periodontal therapy were not observed in lactoferrin concentrations in whole saliva 32 .…”

Section: Discussionmentioning

confidence: 74%

“…Several studies reported that the levels of distinct salivary proteins are altered in individuals with PD 10,14-19,32 . Therefore, the present study was undertaken to investigate and compare the pattern of secretion and the expression of MG2 and lactoferrin in individuals with aggressive periodontitis, chronic periodontitis and without periodontitis.…”

Section: Introductionmentioning

confidence: 99%

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Differential expression of salivary glycoproteins in aggressive and chronic periodontitis

et al. 2012

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Objectives The aim of this study was to compare the pattern of secretion and the expression of mucin glycoprotein-2 (MG2) and lactoferrin in individuals with or without periodontitis. Material and Methods Five individuals with aggressive periodontitis (APG), 5 with generalized chronic periodontitis (CPG) and 5 without periodontitis (CG) were enrolled after informed consent. Non-stimulated and stimulated submandibular and sublingual saliva was collected and samples analyzed by Western blot probed with specific antibodies. Results Stimulated and non-stimulated salivary flow rates did not differ among groups. Western blot analysis revealed that stimulation led to: an increase in MG2 expression in all groups, and to lactoferrin expression in APG and CPG. In non-stimulated saliva, CG exhibited the highest expression of both glycoproteins. In stimulated saliva, CG exhibited the highest expression of MG2, whereas APG the highest of lactoferrin. Conclusions The pattern of secretion of MG2 and lactoferrin in health and disease is complex. Although the present study analyzed samples from a limited number of participants, the reduced expression of MG2 and lactoferrin in APG and CPG under non-stimulated condition, the predominant circumstance of salivary secretion during the day, suggests that these salivary constituents may play a role in the etiopathogenesis of these diseases.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

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Differential expression of salivary glycoproteins in aggressive and chronic periodontitis

et al. 2012

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“…Indeed, saliva is swallowed at the rate of ~1 mL per minute. The constituent antimicrobial proteins and peptides of whole saliva include cystatins [34] and histatins [35,36], lysozyme, lactoferrin and lactoperoxidase [37], defensins [38], cathelicidin [39], and calprotectin (S100A8/A9) [40]. The antimicrobial proteins/peptides all likely limit the overgrowth of many species in the dental biofilm.…”

Section: Characteristics Of Different Oral Ecological Nichesmentioning

confidence: 99%

The oral microbiome and the immunobiology of periodontal disease and caries

2014

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The composition of the oral microbiome differs from one intraoral site to another, reflecting in part the host response and immune capacity at each site. By focusing on two major oral infections, periodontal disease and caries, new principles of disease emerge. Periodontal disease affects the soft tissues and bone that support the teeth. Caries is a unique infection of the dental hard tissues. The initiation of both diseases is marked by an increase in the complexity of the microbiome. In periodontitis, pathobionts and keystone pathogens such as Porphyromonas gingivalis appear in greater proportion than in health. As a keystone pathogen, P. gingivalis impairs host immune responses and appears necessary but not sufficient to cause periodontitis. Historically, dental caries had been causally linked to Streptococcus mutans. Contemporary microbiome studies now indicate that singular pathogens are not obvious in either caries or periodontitis. Both diseases appear to result from a perturbation among relatively minor constituents in local microbial communities resulting in dysbiosis. Emergent consortia of the minor members of the respective microbiomes act synergistically to stress the ability of the host to respond and protect. In periodontal disease, host protection first occurs at the level of innate gingival epithelial immunity. Secretory IgA antibody and other salivary antimicrobial systems also act against periodontopathic and cariogenic consortia. When the gingival immune response is impaired, periodontal tissue pathology results when matrix metalloproteinases are released from neutrophils and T cells mediate alveolar bone loss. In caries, several species are acidogenic and aciduric and appear to work synergistically to promote demineralization of the enamel and dentin. Whereas technically possible, particularly for caries, vaccines are unlikely to be commercialized in the near future because of the low morbidity of caries and periodontitis.

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“…Comparison of the behaviour of salivary proteases (not including collagenase) and glycosidases in patients treated for periodontitis revealed that although the levels of both types of enzymes declined as a result of treatment, they did not do so simultaneously [147]. Two smaller studies of the effect of periodontal treatment on salivary antibacterial proteins have yielded inconsistent data [148, 149]. There is, as yet, no evidence that salivary glutathione peroxidase is altered by treatment in periodontal disease [150].…”

Section: Protein Biomarkers Of Periodontitis: Candidate Protein Stmentioning

confidence: 99%

Protein Biomarkers of Periodontitis in Saliva

Taylor

2014

ISRN Inflammation

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Periodontitis is a chronic inflammatory condition of the tissues that surround and support the teeth and is initiated by inappropriate and excessive immune responses to bacteria in subgingival dental plaque leading to loss of the integrity of the periodontium, compromised tooth function, and eventually tooth loss. Periodontitis is an economically important disease as it is time-consuming and expensive to treat. Periodontitis has a worldwide prevalence of 5–15% and the prevalence of severe disease in western populations has increased in recent decades. Furthermore, periodontitis is more common in smokers, in obesity, in people with diabetes, and in heart disease patients although the pathogenic processes underpinning these links are, as yet, poorly understood. Diagnosis and monitoring of periodontitis rely on traditional clinical examinations which are inadequate to predict patient susceptibility, disease activity, and response to treatment. Studies of the immunopathogenesis of periodontitis and analysis of mediators in saliva have allowed the identification of many potentially useful biomarkers. Convenient measurement of these biomarkers using chairside analytical devices could form the basis for diagnostic tests which will aid the clinician and the patient in periodontitis management; this review will summarise this field and will identify the experimental, technical, and clinical issues that remain to be addressed before such tests can be implemented.

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Peroxidases, Iactoferrin and lysozyme in peripheral blood neutrophils, gingival crevicular fluid and whole saliva of patients with localized juvenile periodontitis

Cited by 25 publications

References 30 publications

Differential expression of salivary glycoproteins in aggressive and chronic periodontitis

Differential expression of salivary glycoproteins in aggressive and chronic periodontitis

The oral microbiome and the immunobiology of periodontal disease and caries

Protein Biomarkers of Periodontitis in Saliva

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