Peroxiredoxin 4, a new oxidative stress marker in follicular fluid, may predict in vitro fertilization and embryo transfer outcomes

Abstract

Background: Peroxiredoxin 4 (Prdx4), a member of the Prdx family, can catalyze the reduction of reactive oxygen species. This study aims to explore whether Prdx4 can serve as an effective marker in follicular fluid (FF) for predicting in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycle outcomes.Methods: In this prospective study, all participants were recruited from the center of clinical reproductive medicine from 2017 September to 2018 December. Women with tubal or male factor infertility undergoing their first IVF/ICSI cycle were recruited (n=138). FF samples from each patient were collected on the day of oocyte retrieval. Prdx4 concentrations were measured, and the correlation between Prdx4 levels and IVF outcomes was analyzed. Results:The results showed that pregnant women had higher levels of Prdx4 than nonpregnant women.Prdx4 was positively correlated with the oocyte fertilization rate (r =0.334; P=0.011) and good quality embryo rate (r =0.326; P=0.013). Furthermore, we found that the clinical pregnancy rate was positively correlated with Prdx4 levels in a concentration-dependent manner in the Prdx4 quartiles (<13.38, 13.83-16.93, 16.93-22.93, >22.93 ng/mL). The fertilization rates, clinical pregnancy rates and live pregnancy rates were all significantly higher in the highest Prdx4 quartile group than in the lowest quartile. Moreover, the results indicated that Prdx4 had an area under the receiver operating characteristic curve (AUC) of 0.754, corresponding to an optimal cutoff point of 22.30 ng/mL. Conclusions: Our results provide evidence that higher expression of antioxidants, such as Prdx4, in the FF of IVF patients tends to indicate a higher likelihood of pregnancy through an oocyte quality mechanism.

Development and characterization of human fetal female reproductive tract organoids to understand Müllerian duct anomalies

“…NEDD4 is a known modulator of Wnt signaling and controls proliferation and self-renewal of intestinal stem cells ( 36 ). Peroxiredoxin-4 (PRDX4) is an antioxidant protein that is highly enriched in human follicular fluid ( 37 ). Expectedly, PRDX4 was down-regulated in fetal FT organoids compared with the adult ones ( Fig.…”

Association of Prx4, Total Oxidant Status, and Inflammatory Factors with Insulin Resistance in Polycystic Ovary Syndrome

“…Peroxiredoxins (Prxs) were first identified in 1994, as a new class of peroxidase; they belong to the family of cysteine-dependent peroxidase enzymes. They scavenge peroxynitrite and peroxide in the mammalian cells [ 18 , 19 ]. Prxs have six members; they can remove more than 90% of cellular peroxides [ 20 ].…”

Gene Sequence of Rubella Virus Infection in Serum of Wives That Correlate to Intrauterine Insemination Success Rate in Baghdad's Population Couples

“…In spite of substantial progresses in the assisted reproductive technology (ART), there is a remarkable low rate of success in this method. Several factors are shown to affect the outcomes of IUI and ART (17). Oxidative stress is regarded as one of the most essential factors which influence different IUI and ART procedure steps and its unavoidable outcomes (4).…”