Peroxisome development in yeast is associated with the formation of Pex3-dependent peroxisome-vacuole contact sites

Wu, Huala; Boer, Rinse de; Krikken, Arjen M; Akşit, Arman; Yuan, Wei; Klei, Ida J. van der

doi:10.1016/j.bbamcr.2018.08.021

Cited by 33 publications

(36 citation statements)

References 51 publications

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“…Cells were grown in media containing glucose (peroxisome repressing conditions). At these conditions the cells generally contain a single small peroxisome, associated to the ER (Wu et al, 2018). As shown in Fig.…”

Section: All H Polymorpha Pex23 Family Members Localize To the Ermentioning

confidence: 88%

Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation

Wu¹,

Boer²,

Krikken³

et al. 2020

Preprint

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Two Hansenula polymorpha ER proteins, Pex24 and Pex32, are tethers at peroxisome-ER contacts and function together with the peroxisomal protein Pex11. Their absence disturbs these contacts leading to multiple peroxisomal defects, which can be restored by an artificial tether. AbstractWe analyzed all four Pex23 family proteins of the yeast Hansenula polymorpha, which localize to the ER. Of these Pex24 and Pex32, but not Pex23 and Pex29, accumulate at peroxisome-ER contacts, where they are important for normal peroxisome biogenesis and proliferation and contribute to organelle positioning and segregation.Upon deletion of PEX24 and PEX32 -and to a lesser extent of PEX23 and PEX29peroxisome-ER contacts are disrupted, concomitant with peroxisomal defects. These defects are suppressed upon introduction of an artificial peroxisome-ER tether.

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Section: All H Polymorpha Pex23 Family Members Localize To the Ermentioning

confidence: 88%

Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation

Wu¹,

Boer²,

Krikken³

et al. 2020

Preprint

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“…Recent studies in H. polymorpha revealed that, at conditions of rapid expansion of the peroxisomal membrane, extensive contacts are formed with vacuoles . FM revealed that, under these conditions, Pex3‐GFP accumulates in patches at peroxisome–vacuole contacts.…”

Section: Alternative Functions Of Pex3 and Pex19mentioning

confidence: 99%

The peroxisome biogenesis factors Pex3 and Pex19: multitasking proteins with disputed functions

Jansen

Klei

2019

FEBS Letters

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The peroxisomal membrane protein (PMP) Pex3 and its cytosolic interaction partner Pex19 have been implicated in peroxisomal membrane biogenesis. Although these peroxins have been extensively studied, no consensus has been reached yet on how they operate. Here, we discuss two major models of their function, namely, in direct insertion of proteins into the peroxisomal membrane or in formation of PMP‐containing vesicles from the endoplasmic reticulum (ER). Pex3 can also recruit other proteins to the peroxisomal membrane (e.g., Inp1, Atg30, Atg36), thereby fulfilling roles in other processes such as autophagy and organelle retention. Recent studies indicate that Pex3 and Pex19 can also facilitate sorting of certain membrane proteins to other cellular organelles, including the ER, lipid droplets, and mitochondria.

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“…In N. crassa , WSC plays a role in cortical association of WBs . We recently showed that in glucose‐grown H. polymorpha WT cells, peroxisomes associate with the plasma membrane and cortical ER . Electron microscopy (EM) analysis revealed that in Hp32g403‐deficient cells, peroxisomes remain localized in close vicinity to the plasma membrane and cortical ER (Fig.…”

Section: Resultsmentioning

confidence: 97%

Hansenula polymorpha Pex37 is a peroxisomal membrane protein required for organelle fission and segregation

et al. 2019

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Here, we describe a novel peroxin, Pex37, in the yeast Hansenula polymorpha. H. polymorpha Pex37 is a peroxisomal membrane protein, which belongs to a protein family that includes, among others, the Neurospora crassa Woronin body protein Wsc, the human peroxisomal membrane protein PXMP2, the Saccharomyces cerevisiae mitochondrial inner membrane protein Sym1, and its mammalian homologue MPV17. We show that deletion of H. polymorpha PEX37 does not appear to have a significant effect on peroxisome biogenesis or proliferation in cells grown at peroxisome‐inducing growth conditions (methanol). However, the absence of Pex37 results in a reduction in peroxisome numbers and a defect in peroxisome segregation in cells grown at peroxisome‐repressing conditions (glucose). Conversely, overproduction of Pex37 in glucose‐grown cells results in an increase in peroxisome numbers in conjunction with a decrease in their size. The increase in numbers in PEX37‐overexpressing cells depends on the dynamin‐related protein Dnm1. Together our data suggest that Pex37 is involved in peroxisome fission in glucose‐grown cells. Introduction of human PXMP2 in H. polymorpha pex37 cells partially restored the peroxisomal phenotype, indicating that PXMP2 represents a functional homologue of Pex37. H.polymorpha pex37 cells did not show aberrant growth on any of the tested carbon and nitrogen sources that are metabolized by peroxisomal enzymes, suggesting that Pex37 may not fulfill an essential function in transport of these substrates or compounds required for their metabolism across the peroxisomal membrane.

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Peroxisome development in yeast is associated with the formation of Pex3-dependent peroxisome-vacuole contact sites

Cited by 33 publications

References 51 publications

Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation

Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation

The peroxisome biogenesis factors Pex3 and Pex19: multitasking proteins with disputed functions

Hansenula polymorpha Pex37 is a peroxisomal membrane protein required for organelle fission and segregation

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