Persistence, dissipation behavior and dietary risk assessment of a combi-product of chlorantraniliprole and λ-cyhalothrin in/on pigeonpea (Cajanus cajan L.)

Abstract

Pigeonpea ( Cajanus cajan L.) is an annually cultivated food and forage crop, attacked by a large number of pests mainly pod borer ( Helicoverpa armigera ). For the control of this insect pest, a combination of broad-spectrum insecticides viz., chlorantraniliprole and λ-cyhalothrin have been in use to reduce the pod damage and increase crop production worldwide. Therefore, a field trial was conducted to study dissipation and persistence behavior of insecticides combination (Chlorantraniliprole 9.26 % + λ-cyhalothrin 4.63 % ZC) in/on pigeonpea at the recommended dose (RD) 30 g a.i./ha and double recommended dose 60 g a.i./ha (2RD). The quantitative analysis was performed using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) for chlorantraniliprole and Gas chromatography with electron captured detector (GC-ECD) for λ-cyhalothrin. The Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method was validated for its accuracy, precision and sensitivity. The chlorantraniliprole persisted upto 30 days and λ-cyhalothrin persisted upto 10 days at RD and 20 days at 2RD. The half-lives (DT 50 ) of chlorantraniliprole and λ-cyhalothrin were from 4.95 to 5.78 days and 2.48–4.33 days at recommended and double recommended dose in pigeonpea, respectively. However, residues of both insecticides measured from soil at 30 th day and harvest time were below the limit of quantification (LOQ). The waiting period deciphered for chlorantraniliprole and λ-cyhalothrin applied as combi-product was 9 days at recommended dose. Dietary risk assessment (Risk quotient<1) was performed on the basis of field trial suggested the application of chlorantraniliprole and λ-cyhalothrin as combi-product at recommended dose is safe for the consumers.

UHPLC-MS/MS and QRT-PCR profiling of PGP agents and Rhizobium spp. of induced phytohormones for growth promotion in mungbean (var. Co4)

“…The various concentrations (especially in the linear dynamic range) were recorded to determine the analytical method's limit of detection (LOD) and limit of quantitation (LOQ). The recovery of phytohormones was used to assess the accuracy and precision ( Kansara et al., 2021 ).…”

Diamide Insecticides: Efficacy, Toxicity and Analytical Methods for Residue Monitoring in Food Samples

“…The results showed that chlorantraniliprole persisted up to 30 days at RD and 2RD with the half-lives (t1/2) of chlorantraniliprole ranged from 4.95 to 5.78 days at RD and 2RD in pigeonpea, respectively. Residues of the insecticide were below LOQ when measured from the soil on the 30th day [29].…”

“…RQ was estimated for the residues determined directly on the treated pigeonpea plants sprayed with an insecticide containing a combination from chlorantraniliprole and λ-cyhalothrin at 30 and 60 g a.i. ha -1 [29]. Their results indicated that dietary risk assessment (RQ <1), which suggested that the application of the combination at RD is safe for the consumers.…”

“…Their results indicated that dietary risk assessment (RQ <1), which suggested that the application of the combination at RD is safe for the consumers. In the same study, the estimated daily intake (EDI) was calculated for chlorantraniliprole and λ-cyhalothrin residue by multiplying the average food consumption rate (g day -1 ) with the product of pesticide concentration (mg kg -1 ) divided by the mean body weight of different groups of Indian consumers (kg) [29]. Also, they assessed the long-term risk assessment of intakes compared to pesticide toxicological data by calculating the RQ, dividing the EDI by the relevant acceptable daily intake (ADI) expressed in mg kg -1 bw day -1 .…”

Impact of Partial Shades on Bacopa monnieri L. for Nootropic Parameters

“…The lowest addition level of the analysis method was designated as the limit of quantification (LOQ) of both bacopasides and bacoside throughout the experiment, and the limit of detection (LOD) was estimated as three times the signal-to-noise ratio (S/N). For LOD and LOQ calculation of all three compounds were calculated by using the following formulas: (a) LOD (µg/kg) = (mean of standard deviation/Slope) × 3; (b) LOQ (µg/kg) = (mean of standard deviation/Slope) × 10 [11,12]. A recovery study was used to assess the accuracy and precision.…”