Persistent attenuation of brain oxidative stress through aging in perinatal maternal separated rat pups supplemented with choline and docosahexaenoic acid or Clitoria ternatea aqueous root extract

Almeida, Prathibha Maria D; Kamath, Shobha; Shenoy, Pooja R; Bernhardt, Liegelin Kavitha; Kishore, Anoop; Sesappa, Kiranmai

doi:10.5114/fn.2018.78702

Cited by 10 publications

(5 citation statements)

References 49 publications

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“…The method is suitable for MDA quantification from a rodent’s brain and in studies that aim for the measurement and estimation of oxidative stress in different treatments and induced pathologies. Unlike the classic spectrophotometric methods [ 24 , 25 ], this method is superior in terms of sensitivity and specificity; the interferences of other compounds capable of absorption at 532 nm in VIS is avoided. Moreover, this method is simple and cost-effective; it does not imply the multiple steps of preparation for analytical extraction that may lead to interactions.…”

Section: Discussionmentioning

confidence: 99%

A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain

et al. 2021

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In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 6) (B). Isocratic elution was 14% for (A) and 86% for (B). The injection volume (loop mode) was 100 μL with an analysis time of 1.5 min. Flow rate was set at 1 mL/min. The eluted compound was detected at 532 nm by a DAD detector by keeping the column oven at room temperature. The results indicated that the method has good linearity in the range of 0.2–20 μg/g. Both intra- and inter-day precision, expressed as RSD, were ≤15% and the accuracies ranged between ±15%. The lower limit of quantification (LLOQ), stability, and robustness were evaluated and satisfied the validation criteria. The method was successfully applied in a study of chronic toxicology following different treatment regimens with haloperidol and metformin.

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Section: Discussionmentioning

confidence: 99%

A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain

et al. 2021

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“…We found no damage to lipids in the hippocampus and hypothalamus of early‐weaned female pups; however, the levels of lipid peroxidation in the hippocampus of female pups are higher than male. Studies have shown that the brain of male pups subjected to maternal separation early in life presents an imbalance of redox state and increased lipid peroxidation (Almeida et al., 2018; Daniels, Marais, Stein, & Russell, 2012; Uysal et al., 2005). Taking together, these findings suggest that male offspring are more susceptible than female, but we cannot rule out that other biomolecules can be damaged by reactive species.…”

Section: Discussionmentioning

confidence: 99%

Early weaning alters redox status in the hippocampus and hypothalamus of rat pups

Klein

August

Santos

et al. 2020

Intl J of Devlp Neuroscience

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Exposure to environmental factors can program the metabolism, conferring resistance or increasing the risk to chronic disease development in childhood and adulthood. In this sense, lactation is an important period in this window of development. Herein, we investigated the effect of early weaning on neurochemical and behavioral changes in offspring at weaning and adulthood. Female and male pups were divided into four groups: (1) Control weaning (weaning on the PND21, pups were kept with the biological mother); (2) Early Weaning Bromocriptine group (EWB) (pharmacological weaning on PND16); (3) Early Weaning Cross-Fostering group (EWCF) (pups housed with a foster mother on PND16 up to PND21); (4) Early Weaning Without Care group (EWWC) (weaning on PND16, maternal separation). Weight control of pups was recorded from postnatal Day 16 to 59. On the 21st day, part of the pups was euthanized and the hippocampus and hypothalamus were removed for biochemical evaluation. The remaining pups were submitted to behavioral tests on the 60th postnatal day. Early weaning reduced the pups' body weight, in a sexdependent way. At 60 days of age, male pups of EWCF and EWWC groups have lower body weight compared to control male, and female body weight was lower than male pups. In relation to biochemical changes in the brain, weaning altered the levels of oxidants, increased the enzymatic activity of superoxide dismutase (SOD), and glutathione peroxidase (GPx), as well as induced lipid peroxidation. Weaning was also able to alter long-term memory and induce anxious behavior in pups. Our results demonstrate that the different types of early weaning changed the parameters of redox status in the hippocampus and hypothalamus of pups (21 days old), suggesting a prooxidative profile, in addition, to alter learning/memory and inducing an anxious behavior in male offspring (60 days old).

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“… 19 Among malnourished pigs, addition of dietary DHA, methyl donor nutrients including choline, or both attenuated losses in fetal brain weight compared with controls. 103 Combined choline and DHA administration decreases brain inflammation 104 and oxidative stress 105 in mouse models. In fact, these nutrients may work synergistically: offspring of dams supplemented with choline and DHA had more hippocampal neurons than those given either nutrient alone.…”

Section: Methodsmentioning

confidence: 99%

Choline and docosahexaenoic acid during the first 1000 days and children’s health and development in low- and middle-income countries

2021

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Choline and DHA are nutrients that, when provided during the first 1000 days from conception to age 2 years, may have beneficial effects on child neurodevelopment as well as related health factors, including birth outcomes and child growth, morbidity, and inflammation. Because these nutrients are found mainly in animal-source foods, they may be lacking in the diets of pregnant and lactating women and young children in low- and middle-income countries, potentially putting children at risk for suboptimal development and health. Prior reviews of these nutrients have mainly focused on studies from high-income countries. Here, a narrative review is presented of studies describing the pre- and postnatal roles of choline, docosahexaenoic acid, and a combination of the 2 nutrients on child neurodevelopment, birth outcomes, growth, morbidity, and inflammation in low- and middle-income countries. More studies are needed to understand the specific, long-term effects of perinatal choline and docosahexaenoic acid intake in various contexts.

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Persistent attenuation of brain oxidative stress through aging in perinatal maternal separated rat pups supplemented with choline and docosahexaenoic acid or Clitoria ternatea aqueous root extract

Cited by 10 publications

References 49 publications

A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain

A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain

Early weaning alters redox status in the hippocampus and hypothalamus of rat pups

Choline and docosahexaenoic acid during the first 1000 days and children’s health and development in low- and middle-income countries

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