Persistent Baculovirus Infection Results from Deletion of the Apoptotic Suppressor Gene p35

Lee, Jin‐Ching; Chen, Hong Hwa; Chao, Yu-Chan

doi:10.1128/jvi.72.11.9157-9165.1998

Cited by 28 publications

(9 citation statements)

References 44 publications

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“…Otherwise, we used another virus whose whole genome had been sequenced to represent that family. We also included viruses known to be able to infect S. frugiperda , such as Sf-rhabdovirus, as well as viruses known to contaminate insect cell cultures, such as Bombyx mori macula-like latent virus [ 27 – 30 ].…”

Section: Resultsmentioning

confidence: 99%

A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production

Geisler¹

2018

BMC Biotechnol

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BackgroundAdventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively parallel sequencing technology. Typically, this involves mapping or BLASTN searching individual reads against viral nucleotide databases. Although extremely sensitive for known viruses, this approach can easily miss viruses that are too dissimilar to viruses in the database. Moreover, it is computationally intensive and requires reference cell genome databases. To avoid these drawbacks, we set out to develop an alternative approach. We reasoned that searching genome and transcriptome assemblies for adventitious viral contaminants using TBLASTN with a compact viral protein database covering extant viral diversity as the query could be fast and sensitive without a requirement for high performance computing hardware.ResultsWe tested our approach on Spodoptera frugiperda Sf-RVN, a recently isolated insect cell line, to determine if it was contaminated with one or more adventitious viruses. We used Illumina reads to assemble the Sf-RVN genome and transcriptome and searched them for adventitious viral contaminants using TBLASTN with our viral protein database. We found no evidence of viral contamination, which was substantiated by the fact that our searches otherwise identified diverse sequences encoding virus-like proteins. These sequences included Maverick, R1 LINE, and errantivirus transposons, all of which are common in insect genomes. We also identified previously described as well as novel endogenous viral elements similar to ORFs encoded by diverse insect viruses.ConclusionsOur results demonstrate TBLASTN searching massively parallel sequencing (MPS) assemblies with a compact, manually curated viral protein database is more sensitive for adventitious virus detection than BLASTN, as we identified various sequences that encoded virus-like proteins, but had no similarity to viral sequences at the nucleotide level. Moreover, searches were fast without requiring high performance computing hardware. Our study also documents the enhanced biosafety profile of Sf-RVN as compared to other Sf cell lines, and supports the notion that Sf-RVN is highly suitable for the production of safe biologicals.Electronic supplementary materialThe online version of this article (doi: 10.1186/s12896-017-0412-z) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production

Geisler¹

2018

BMC Biotechnol

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“…Insect cell culture. The Spodoptera frugiperda IPLB-Sf21 (Sf21) insect cell line was cultured as a monolayer in TNM-FH insect medium, containing 8% heat-inactivated fetal bovine serum as described previously [18]. It was used for the propagation and infection of recombinant baculoviruses; titers of viruses were determined by a newly developed quantitative real-time PCR-based method [19].…”

Section: Methodsmentioning

confidence: 99%

Assembly of human severe acute respiratory syndrome coronavirus-like particles

Lin

Liu

et al. 2004

Biochemical and Biophysical Research Communications

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114

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Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. We found that the membrane and envelope proteins are sufficient for the efficient formation of virus-like particles and could be visualized by electron microscopy. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. The construction of engineered virus-like particles bearing resemblance to the authentic one is an important step towards the development of an effective vaccine against infection of SARS CoV.

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“…However, Lee et al . reported that latency can be caused by the infection of Spodoptera frugiperda (Sf) cells with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) that lack the anti-apoptosis gene p 35 [ 143 ]. Notably, even during the latency, infectious AcMNPV particles were continuously produced.…”

Section: Virology Of the Hytrosavirusesmentioning

confidence: 99%

Virology, Epidemiology and Pathology of Glossina Hytrosavirus, and Its Control Prospects in Laboratory Colonies of the Tsetse Fly, Glossina pallidipes (Diptera; Glossinidae)

Kariithi

Oers

Vlak

et al. 2013

Insects

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The Glossina hytrosavirus (family Hytrosaviridae) is a double-stranded DNA virus with rod-shaped, enveloped virions. Its 190 kbp genome encodes 160 putative open reading frames. The virus replicates in the nucleus, and acquires a fragile envelope in the cell cytoplasm. Glossina hytrosavirus was first isolated from hypertrophied salivary glands of the tsetse fly, Glossina pallidipes Austen (Diptera; Glossinidae) collected in Kenya in 1986. A certain proportion of laboratory G. pallidipes flies infected by Glossina hytrosavirus develop hypertrophied salivary glands and midgut epithelial cells, gonadal anomalies and distorted sex-ratios associated with reduced insemination rates, fecundity and lifespan. These symptoms are rare in wild tsetse populations. In East Africa, G. pallidipes is one of the most important vectors of African trypanosomosis, a debilitating zoonotic disease that afflicts 37 sub-Saharan African countries. There is a large arsenal of control tactics available to manage tsetse flies and the disease they transmit. The sterile insect technique (SIT) is a robust control tactic that has shown to be effective in eradicating tsetse populations when integrated with other control tactics in an area-wide integrated approach. The SIT requires production of sterile male flies in large production facilities. To supply sufficient numbers of sterile males for the SIT component against G. pallidipes, strategies have to be developed that enable the management of the Glossina hytrosavirus in the colonies. This review provides a historic chronology of the emergence and biogeography of Glossina hytrosavirus, and includes researches on the infectomics (defined here as the functional and structural genomics and proteomics) and pathobiology of the virus. Standard operation procedures for viral management in tsetse mass-rearing facilities are proposed and a future outlook is sketched.

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Persistent Baculovirus Infection Results from Deletion of the Apoptotic Suppressor Gene p35

Cited by 28 publications

References 44 publications

A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production

A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production

Assembly of human severe acute respiratory syndrome coronavirus-like particles

Virology, Epidemiology and Pathology of Glossina Hytrosavirus, and Its Control Prospects in Laboratory Colonies of the Tsetse Fly, Glossina pallidipes (Diptera; Glossinidae)

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