Perspectives and Prospects for Whole Cell Catalysis

Domach, Michael M.

doi:10.1007/s10562-014-1389-z

Cited by 9 publications

(18 citation statements)

References 73 publications

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“…For centuries, whole‐cell biotransformations have been used in the production of food and beverages. More recently, whole‐cell biocatalysis has become an active area of research due to its potential for low‐cost synthesis of fine chemicals, including chiral molecules and pharmaceuticals, natural food additives, and applications in bioremediation and energy . Whole‐cell biocatalysis benefits from complex multi‐enzyme reaction steps, applicability at ambient conditions, and attractive properties such as self‐repair and regeneration.…”

Section: Figurementioning

confidence: 99%

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

et al. 2019

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A common kinetic framework for studies of whole‐cell catalysis is vital for understanding and optimizing bioflow reactors. In this work, we demonstrate the applicability of a flow‐adapted version of Michaelis‐Menten kinetics to an electrocatalytic bacterial biofilm. A three‐electrode microfluidic biofilm flow reactor measured increased turnover rates by as much as 50 % from a Geobacter sulfurreducens biofilm as flow rate was varied. Based on parameters from the applied kinetic framework, flow‐induced increases to turnover rate, catalytic efficiency and device reaction capacity could be linked to an increase in catalytic biomass. This study demonstrates that a standardized kinetic framework is critical for quantitative measurements of new living catalytic systems in flow reactors and for benchmarking against well‐studied catalytic systems such as enzymes.

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Section: Figurementioning

confidence: 99%

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

et al. 2019

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“…Asymmetric reduction of prochiral ketone via biocatalysis is one of the most promising routes to prepare optically active alcohols. [1][2][3][4][5][6] Molecules with tetralone rings are used for the synthesis of many biological active and drug precursors. For example, Sertraline with a tetralone ring is used to treat disorder such as obsessive compulsive, post-traumatic stress, premenstrual dysphoric, and social anxiety.…”

Section: Introductionmentioning

confidence: 99%

“…Biocatalysts are increasingly used as efficient and environmentally safe alternatives than traditional catalysts in the production of fine chemicals, difficult to make using conventional organic catalysts. 4,[16][17][18][19] Mohammadi et al found that (S)-1-tetralol (S)-2 was obtained in 75% yield and 91% enantiomeric excess (ee) using Brassica oleracea whole-cell biocatalyst. 20 Similarly, Yadav et al reported that Daucus carota exhibited good performance in the asymmetric reduction of 1 to (S)-2 in terms of enantioselectivity (96% ee), but low conversion (70%) and yield (52%).…”

Section: Introductionmentioning

confidence: 99%

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Biocatalytic asymmetric synthesis of (R)‐1‐tetralol using Lactobacillus paracasei BD101

Kalay

Şahin

2021

Chirality

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Asymmetric bioreduction of ketones is a fundamental process in the production of organic molecules. Compounds containing tetralone rings are found in the structure of many biologically active and pharmaceutical molecules. Biocatalytic reduction of ketones is one of the most promising and significant routes to prepare optically active alcohols. In this study, the reductive capacity of Lactobacillus paracasei BD101 was investigated as whole-cell biocatalyst in the enantioselective reduction of 1-tetralone (1). In biocatalytic reduction reactions, the conversion of the substrate and the enantiomeric excess (ee) of the product are significantly affected by optimization parameters such as temperature, agitation rate, pH, and incubation time. Effects of these parameters on ee and conversion were investigated comprehensively. (R)-1-tetralol ((R)-2), which can be used to treat disorder such as obsessive compulsive, posttraumatic stress, premenstrual dysphoric, and social anxiety, was manufactured in enantiopure form, high yield and gram-scale, using whole-cell biocatalysts of L. paracasei BD101. The 7.04 g of (R)-2 was obtained in optically pure form with 95% yield. Also, to our knowledge, this is the first report on production of (R)-2 using whole-cell biocatalyst in excellent yield, conversion, enantiopure form and gram scale. This is a clean, eco-friendly and cheap method for the synthesis of (R)-2 compared with chemical catalyst.

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“…However, some studies indicate that inclusion bodies produced by fusion of the target enzymes and the coiled-coil domain or aggregation-prone protein in Escherichia coli can retain some enzyme functions (Arié, Miot, Sassoon, & Betton, 2006;Diener, Kopka, Pohl, Jaeger, & Krauss, 2016;Jäger et al, 2018). Now, functional inclusion bodies begin to appear in biocatalysis (Domach, 2015;Kloss et al, 2018aKloss et al, , 2018b. For example, the activity of benzaldehyde lyase in functional inclusion bodies formed by using the domain 3HAMP (histidine kinases, adenylyl cyclases, methylaccepting chemotaxis proteins, and phosphatases) as fusion tags is three times higher than that of soluble enzyme for the synthesis of (R)benzoins (Kloss et al, 2018a).…”

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confidence: 99%

Enzyme assembly guided by SPFH‐induced functional inclusion bodies for enhanced cascade biocatalysis

Jin

Zhang

et al. 2020

Biotech & Bioengineering

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Enzyme clustering into compact agglomerates could accelerate the processing of intermediates to enhance metabolic pathway flux. However, enzyme clustering is still a challenging task due to the lack of universal assembly strategy applicable to all enzymes. Therefore, we proposed an alternative enzyme assembly strategy based on functional inclusion bodies. First, functional inclusion bodies in cells were formed by the fusion expression of stomatin/prohibitin/flotillin/HflK/C (SPFH) domain and enhanced green fluorescent protein, as observed visually and by transmission electron microscopy. The formation of SPFH‐induced functional inclusion bodies enhanced intermolecular polymerization as revealed by further analysis combined with Förster resonance energy transfer and bimolecular fluorescent complimentary. Finally, the functional inclusion bodies significantly improved the enzymatic catalysis in living cells, as proven by the examples with whole‐cell biocatalysis of phenyllactic acid by Escherichia coli, and the production of N‐acetylglucosamine by Bacillus subtilis. Our findings suggest that SPFH‐induced functional inclusion bodies can enhance the cascade reaction of enzymes, to serve as a potential universal strategy for the construction of efficient microbial cell factories.

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Perspectives and Prospects for Whole Cell Catalysis

Cited by 9 publications

References 73 publications

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

Biocatalytic asymmetric synthesis of (R)‐1‐tetralol using Lactobacillus paracasei BD101

Enzyme assembly guided by SPFH‐induced functional inclusion bodies for enhanced cascade biocatalysis

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