Perspectives on improving crop Rubisco by directed evolution

A carboxysome‐based CO2 concentrating mechanism for C3 crop chloroplasts: advances and the road ahead

“…E. coli cell lines have also been used to evolve Rubisco that either possesses a higher catalytic rate, or is more readily foldable (Gleizer et al, 2019;Mueller-Cajar & Whitney, 2008;Wilson et al, 2017;Zhou & Whitney, 2019). This experimental approach, while exciting, is also challenging due to the high rates of 'false-positive' mutants that arise, however the development of new E. coli cell lines will greatly assist work in this field (Gionfriddo et al, 2023).…”

The challenge of engineering Rubisco for improving photosynthesis

“…In plants the RbcL subunit of Rubisco is encoded by the chloroplast DNA (plastome) and is synthesized in the chloroplast stroma, while the nuclear encoded RbcS (1 to 25 paralogs, plant species dependent [3]) is translated in the cytosol and imported into the chloroplast [4,6].…”

“…improve crop yields (food security) [1][2][3][4] and lessen the greenhouse effect of atmospheric CO 2 [5]. Here we discuss two strategies that aim to screen for improved catalytic properties of Rubisco, namely directed evolution (DE) and/or computational protein design.…”

“…The fixation of atmospheric CO in this crucial biological 2 process is mediated by the enzyme Rubisco. The inefficiencies of Rubisco have inspired researchers for decades to explore ways to improve its function with the goal of increasing crop yields [1][2][3][4], and more recently to combat global warming [5]. In this graphical review we highlight the challenges involved in engineering plant Rubisco, with a focus on the extensive chaperone requirement for its biogenesis.…”