Pertumbuhan Planlet Stroberi (Fragaria ananassa D) Var. Dorit pada Beberapa Variasi Media Modifikasi In Vitro di Balai Penelitian Jeruk dan Buah Subtropika (BALITJESTRO)

Nofrianinda, Vida; Yulianti, Farida; Agustina, Eva

doi:10.29080/biotropic.2017.1.1.32-41

Cited by 6 publications

(7 citation statements)

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“…This was presumed by the availability of main macronutrients (Nitrogen (N), Phosphorus (P), Potassium (K)) in the leaf fertilizers that are able to meet the needs of young plants whose cells are still actively dividing. N and P elements are nutrients needed by plants in large quantities (Nofrianinda et al 2017). The element of N has an important role in plant growth, including shoots initiation appearance.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Leaf Fertilizer Media on Multiplication of Barangan Banana (Musa acuminate L.) in vitro

et al. 2021

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Barangan banana (Musa acuminata L.) is a plant that has the biggest contribution to national fruit production. The increasing demand for the banana requires the availability of seedlings, while conventional cultivation is unable to produce healthy, disease-free seedlings in a short time and large amount. In vitro culture is considered to be able to handle the constraints of the conventional seed supply. The purpose of this study was to determine the effect of foliar fertilizers on in vitro multiplication of barangan bananas. The method used was a single factor of Completely Randomized Design (CRD), the addition of leaf fertilizer consisting of five levels of media treatment, media MS (control), and 0.50 ml L-1 (p1); 0.75 ml L-1 (p2); 1.00 ml L-1 (p3); 1.25 ml L-1 (p4); 1.50 ml L-1 (p5) of leaf fertilizer media repeated five times. The data were analyzed with Analysis Variance then followed by Duncan Multiple Ranged Test of 5%. The results showed MS media still gave the best results compared to other leaf fertilizer media. Meanwhile, the leaf fertilizer media used in this study still gave good results on the initial time of buds appearance (p3 treatment), the number of shoots and the number of leaves in the p5 treatment, as well as the height of the plant in the p4 and p5 treatments. Therefore, the leaf fertilizer can be used to substitute MS media

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Section: Discussionmentioning

confidence: 99%

The Influence of Leaf Fertilizer Media on Multiplication of Barangan Banana (Musa acuminate L.) in vitro

et al. 2021

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“…A week-old callus on liquid media remained white or yellowish-white color, then started to turn brown at the 2 nd and 3 rd week (Figure 5, 6, and 7). Browning can be caused by the presence of phenolic compounds, stress due to cutting, or the effect of shaking (Nofrianinda et al, 2017). In liquid media, phenolic compounds are able to dissolve which resulting in the changes of media color into brown.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue culture is a method for isolating part of a plant and maintaining cells or pieces of plant tissue grown on suitable artificial media under aseptic conditions (Nofrianinda et al, 2017). Tissue culture technique is classified into organogenesis and somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

THE EFFECT OF PH AND SUCROSE ON THE EMBRYOGENIC CELLS GROWTH OF SUGAR CANE (Saccharum officinarum) IN LIQUID CULTURE

Aisyah¹,

Widuri²,

Alfina³

et al. 2021

Trop Subtrop Agroecosyst

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Background. Sugarcane (Saccharum officinarum L.) is one of the important commodities in Indonesia as raw material for sugar production. The constraints for sugarcane cultivation are limited availability and poor quality of seedling. The utilization of liquid culture in tissue culture enables to produce good quality seedlings, free of viruses and diseases, and can generate a large quantity in a short time. Liquid culture can produce embryogenic cells faster since embryogenic callus can develop easily in liquid media. Objective. This study determined the appropriate pH and concentration of sucrose in the liquid culture of embryogenic cells. Methodology. This study was conducted in 3 stages of in vitro culture, which were callus induction, solid media proliferation, and liquid media proliferation that using pH and sucrose treatments. Result. The results showed that pH 6.5 + 3% sucrose (A3B1) produced the highest number of callus with the average callus on the first week 29 callus, the second week 8,33 callus and the third week 4,33 callus and the color did not easily turn to brown with color 5Y 8/2. Implications. The protocol was developed that allows embryogenic callus of sugarcane plants to be obtained in liquid media that can be used for embryogenic callus proliferation. Conclusions. pH 6.5 + sucrose 3% (A3B1) was the best combination treatment of pH and sucrose for sugarcane callus proliferation in liquid culture media, which produced the highest number of calluses and color of callus that not easily turned brown.

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“…Ada berbagai media yang dapat digunakan dalam kultur in vitro, Pemilihan medium tergantung dari jenis tanaman yang dipilih. (Nofrianinda et al, 2017). Eksplan (berupa sel, jaringann dan irisan organ) yang ditumbuhkan secara in vitro pada media buatan, juga membutuhkan hara untuk terjadinya morfogenesis dan pertumbuhan (Dwiyani, 2015).…”

Section: Pendahuluanunclassified

PENGARUH KOMBINASI BAP (Benzylamino purin) DAN TDZ (Thidiazuron) TERHADAP PERTUMBUHAN TUNAS TANAMAN PISANG KEPOK TANJUNG (Musa acuminata balbisiana) MELALUI KULTUR IN VITRO

Ayuwira

Hidayat²,

Hendri³

2022

kenanga

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Abstrak : Kultur in vitro merupakan salah satu Teknik yang digunakan untuk mengisolasi berbagai bagian tanaman, seperti protoplas, sel dan jaringan dan organ, yang kemudian ditumbuhkan dalam media secara aseptik. Benih Kepok Tanjung (Musa acuminata balbisiana) masih belum banyak maka dari itu perbanyakan dilakukan secara kultur jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian kombinasi BAP ( Benzylamino purin) dan TDZ ( Thidiazuron ) dalam media tanam terhadap pertumbuhan pisang kepok tanjung (Musa acuminata balbisiana). Penelitian ini dilaksanakan pada tanggal 02 Agustus sampai 15 September 2021 yang bertempat di Laboratorium Kultur Jaringan Dinas Pertanian dan Pangan Aceh Barat Daya. Hasil yang diperoleh dari penelitian ini bahwa pertumbuhan jumlah tunas yang menggunakan zat pengatur tumbuh penggunaan kombinasi BAP (Banzylamino purin) dengan TDZ (Thidiazuron) memiliki pengaruh yang besar terhadap jumlah muncul tunas eksplan dikarenakan BAP dan TDZ dapat memacu dengan cepat perbentukan tunas bila ditambahkan ke media eksplan pisang kepok tanjung (Musa acuminata balbisiana).

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Pertumbuhan Planlet Stroberi (Fragaria ananassa D) Var. Dorit pada Beberapa Variasi Media Modifikasi In Vitro di Balai Penelitian Jeruk dan Buah Subtropika (BALITJESTRO)

Cited by 6 publications

References 0 publications

The Influence of Leaf Fertilizer Media on Multiplication of Barangan Banana (Musa acuminate L.) in vitro

The Influence of Leaf Fertilizer Media on Multiplication of Barangan Banana (Musa acuminate L.) in vitro

THE EFFECT OF PH AND SUCROSE ON THE EMBRYOGENIC CELLS GROWTH OF SUGAR CANE (Saccharum officinarum) IN LIQUID CULTURE

PENGARUH KOMBINASI BAP (Benzylamino purin) DAN TDZ (Thidiazuron) TERHADAP PERTUMBUHAN TUNAS TANAMAN PISANG KEPOK TANJUNG (Musa acuminata balbisiana) MELALUI KULTUR IN VITRO

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