Pertussis: the disease and new diagnostic methods

Friedman, Richard L.

doi:10.1128/cmr.1.4.365

Cited by 64 publications

(45 citation statements)

References 150 publications

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“…Natural infection with B. pertussis is followed by an increase in the concentrations in serum of IgA, IgG, and IgM antibodies to specific antigens as well as to preparations of the whole organism (255,289,293,310,490,519,536,572,601,719,826). Prior to the development of ELISA techniques, the main serologic test for the diagnosis of pertussis was the demonstration of a fourfold increase in agglutinating-antibody titer.…”

Section: Specific Diagnosis Of B Pertussis Infectionsmentioning

confidence: 99%

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

2005

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Bordetella respiratory infections are common in people (B. pertussis) and in animals (B. bronchiseptica). During the last two decades, much has been learned about the virulence determinants, pathogenesis, and immunity of Bordetella. Clinically, the full spectrum of disease due to B. pertussis infection is now understood, and infections in adolescents and adults are recognized as the reservoir for cyclic outbreaks of disease. DTaP vaccines, which are less reactogenic than DTP vaccines, are now in general use in many developed countries, and it is expected that the expansion of their use to adolescents and adults will have a significant impact on reducing pertussis and perhaps decrease the circulation of B. pertussis. Future studies should seek to determine the cause of the unique cough which is associated with Bordetella respiratory infections. It is also hoped that data gathered from molecular Bordetella research will lead to a new generation of DTaP vaccines which provide greater efficacy than is provided by today's vaccines

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Section: Specific Diagnosis Of B Pertussis Infectionsmentioning

confidence: 99%

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

2005

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“…In human pertussis, the from nasal swabs most stages of the disease difficult to culture as the organisms can be isolated frequently during the early and become increasingly paroxysmal stage progresses [15]. In the rat model, the greatest incidence of coughing occurs between days 8 and 14 [4,5], coincident with the time when the bacterial numbers are in rapid decline or the infection has been cleared from the lungs.…”

Section: Resultsmentioning

confidence: 99%

Time-course of infection and responses in a coughing rat model of pertussis

Hall¹,

Parton²,

Wardlaw³

1999

Journal of Medical Microbiology

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Adult female Sprague-Dawley rats were challenged intrabronchially with BordeteZlu pertussis strain 18-323 embedded in fine agarose beads and the time-course of infection and other events was determined. There was a steady decline in the numbers of B. pertussis recovered from the rat lungs, with clearance of the infection in most animals by day 12. Leucocytosis, lung inflammation and an increase in total serum IgE in the rats as a result of the challenge were highest around day 10, which was coincident with the highest incidence of coughing in such animals. IgG and IgA antibodies to the B. pertussis antigens pertussis toxin and filamentous haemagglutinin were not detected until after this period. The coughing rat model of pertussis resembles the human disease in the relationship between the time course of infection and cough production.

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“…A nasopharyngeal swab was obtained frorn each child of this group. The control group consisted of 50 normal children between 3 and 28 months old, from each of whom a nasopharyngeal swab was obtained. Sample processing included culturing in ReganLowe agar and DFA using Bordetellaperiussispolyclonal antibodies and a control strain.…”

Section: Discussionmentioning

confidence: 99%

“…Varios factores diferentes a los anteriormente mencionados contribuyen a la dificultad en el aislamiento de B. pertussis; entre ellos están el sobrecrecimiento en el cultivo de otras especies bacterianas o de hongos de la flora comensal y, por ende, el requerimiento de un medio de cultivo selectivo y, adicionalmente, la falta de experiencia para reconocer el microorganismo (5,8,9).cultivo puede tener unasensibilidad de 80 a 90%, cuando se cumplen las condiciones enumeradas y la ausencia de tratamiento antibiótico previo en el paciente (3).…”

Section: Discussionunclassified

La inmunofluorescencia en el diagnóstico de la tos ferina: experiencia de un laboratorio de referencia

Muñoz¹,

Gómez²,

Agudelo³

1997

biomedica

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ResumenPublicaciones recientes refieren un alto porcentaje de falsos positivos en el diagnóstico de la tos ferina con el empleo de la inmunofluorescencia directa (IFD); por esta razón, se analizaron los datos obtenidos en el Laboratorio de Microbiología del Instituto Nacional de Salud, al emplear esta técnica en el estudio de dos poblaciones infantiles. El primer grupo estaba conformado por267 niños con edades entre 15 días y 7 años, con diagnóstico clínico de síndrome coqueluchoide de etiología por determinar, viral o periussis, y en ellos se tomó un aspirado nasofaríngeo. El grupo control estuvo constituido por 50 niños sanos, entre 3 y 28 meses de edad de quienes se obtuvo una muestra nasofaringea. El procesamiento de las muestras incluyó el cultivo en agar Regan-Lowe y la IFD realizada con el empleo de anticuerpos policlonales anti-Bordetella periussis y una cepa control. Adicionalmente, las muestras del grupo control fueron inocularon en agar sangre de cordero y agar chocolate y los microorganismos recuperados fueron identificados con el empleo de pruebas convencionales. En 36 de las 267 muestras de aspirado nasofaríngeo de los pacientes, se aisló B. periussis y la IFD fue reactiva en 46; en 30 casos las pruebas coincidieron en positividad y en 16 la IFD fue reactiva con el cultivo negativo; esto representó para la IFD una especificidad de 93%. En el grupo control, los cultivos para B. periussis fueron negativos y la IFD fue reactiva con baja intensidad, en 8 (16%) casos, lo que representó una especificidad de 84%. Estos falsos positivos fueron confirmados, por coloración de Gram como Bacillus sp. en 4 casos y, por aislamiento e identificación, como Haernophilus influenzae y Streptococcus pneurnoniae en 2 casos cada uno. Estos datos indican que la IFD puede ser empleada para el diagnóstico de la tosferina, siempre y cuando se tenga especial cuidado en la interpretación de la lectura, basada en la morfología microscópica del microorganismo y en el grado de intensidad de la fluorescencia. Para lograr esto, es indispensable el empleo de cepas control y de personal entrenado en la lectura de la prueba. SummaryRecent publications revealed a high percentage of false positives when the direct fluorescent antibody test (DFA) was used for the diagnosis of pertussis. The aim of this work was to analyse the results obtained in our laboratory when the DFA was used for pertussis diagnosis, in two child-populations. The first group consisted of 267 children, from 15 days to 7 years old, having clinical diagnosis of whooping cough, etiology to be determined. A nasopharyngeal swab was obtained frorn each child of this group. The control group consisted of 50 normal children between 3 and 28 months old, from each of whom a nasopharyngeal swab was obtained. Sample processing included culturing in ReganLowe agar and DFA using Bordetellaperiussispolyclonal antibodies and a control strain.

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Pertussis: the disease and new diagnostic methods

Cited by 64 publications

References 150 publications

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

Time-course of infection and responses in a coughing rat model of pertussis

La inmunofluorescencia en el diagnóstico de la tos ferina: experiencia de un laboratorio de referencia

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