Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis

Vilček, Štefan; Herring, A. J.; Herring, J. A.; Nettleton, P. F.; Lowings, J. P.; Paton, David

doi:10.1007/bf01321060

Cited by 569 publications

(369 citation statements)

References 43 publications

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“…The fact that pestiviruses are serologically cross-reactive and, at the same time, antigenically and genetically diverse [11], makes this suggestion feasible. It has been observed that, due to the genetic differences, the established pan-pestivirus PCR, using primer-pair 324/326 [16] might fail to detect 'HoBi'-like viruses [13]. Moreover, the serological cross-reactivity complicates differentiation through serological assays, as illustrated by the results of our VN studies.…”

Section: Seramentioning

confidence: 88%

Natural infection of cattle with an atypical `HoBi'-like pestivirus – Implications for BVD control and for the safety of biological products

et al. 2007

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-During a study on Bovine Viral Diarrhoea (BVD) epidemiology in Thailand, a pestivirus was detected in serum from a calf. Comparative nucleotide sequence analysis showed that this virus was closely related to a recently described atypical pestivirus (D32/00_'HoBi') that was first isolated from a batch of foetal calf serum collected in Brazil. The results from virus neutralisation tests performed on sera collected from cattle in the herd of the infected calf, showed that these cattle had markedly higher antibody titres against the atypical pestivirus 'HoBi' than against Bovine Viral Diarrhoea Virus types 1 and 2, or Border Disease Virus. The results also supported, consequently, the results from the molecular analysis, and demonstrated that a 'HoBi'-like pestivirus had been introduced to, and was now circulating in the herd. This study is the first to report a natural infection in cattle with a virus related to this atypical pestivirus, and it suggests that this group of pestiviruses may already be spread in cattle populations. The findings have implications for BVD control and for the biosafety of vaccines and other biological products produced with foetal calf serum. Consequently, these atypical pestiviruses should be included in serological assays, and any diagnostic assay aimed at detection of pestiviruses in biological products or animals should be tested for its ability to detect them.BVDV / pestivirus / control / biosafety

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Section: Seramentioning

confidence: 88%

Natural infection of cattle with an atypical `HoBi'-like pestivirus – Implications for BVD control and for the safety of biological products

et al. 2007

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“…A BVDV Antigen ELISA (HerdCheck BVDV Ag/Serum Plus, IDEXX GmbH, Germany) was performed on all blood samples according to the manufacturer's instructions. All results of the Antigen ELISA were verified by RT-PCR as described by Vilcek et al (1994). Blood samples were drawn by jugular vein puncture two weeks and four weeks after birth for validation of the diagnosis and for further investigations.…”

Section: Methodsmentioning

confidence: 99%

“…RT-PCR was carried out on lysed PBMCs (peripheral blood mononuclear cell pellets) of all sheep blood samples, on the blood sample drawn four weeks after birth and organ samples of the calf (lung, kidney, spleen and liver) available after culling (Krametter-Froetscher et al, 2007a). The primers 324 and 326 (Vilcek et al, 1994) amplify a conserved domain at the 5'-end of the viral genome of 288 bp in length.…”

Section: Pestivirus Investigation In a Mixedmentioning

confidence: 99%

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BVDV control program in Austria - is a monitoring of the BDV status in sheep in Austria necessary?

Krametter-Froetscher¹,

Benetka²,

Rasser³

et al. 2009

Vet. Med. - Czech

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ABSTRACT:In cattle referred to the Clinic of Ruminants at the University of Veterinary Medicine Vienna by local veterinarians from Lower Austria, the number of animals positive for antibodies against pestiviruses decreased from 11.9% in 2004 to 7.4% in 2007. In other Austrian regions the seroprevalence of 17.6% in 2004 dropped to 12.2% in 2007. The seroprevalence rates were considerably higher in older animals than in younger indicating a marked decrease of new infections (8.2% in < 1.5 years old animals, 6.8% in 1.5-4.5 years, 19.8% in 4.5-7.5 years and 33.3% in > 7.5 years). These data nevertheless also demonstrate that new pestivirus infections occur, although at a lower rate. We report the case of a calf persistently infected with Border disease virus-3 (BDV-3) detected in a mixed cattle and sheep farm with the status "BVDV-free". Earlier investigations have shown that BDV-3 is endemic in Austrian sheep populations and seems to be a potential risk factor for the reintroduction of pestiviruses in BVDV free cattle herds. Serological findings among the investigated sheep population showed in four out of nine sheep samples considerably higher titres to the BDV strain Moredun than to the BVDV strain NADL. Seroconversion against pestiviruses was also detected in contact cattle and the mother of the persistently infected calf. Pestivirus specific RNA was neither detected in the blood samples collected from the patients of the Clinic for Ruminants nor in the sheep or cattle investigated on the farm described.

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“…Also, viral RNA was extracted from 915 clinical samples (672 whole bloods, 150 feces, 93 nasal swabs) using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA). RT-PCR procedures were performed in a one-tube system using the pan-pestivirus primer pair V324/V326; this system amplifies a portion of the 5'-UTR of pestivirus genomes (Vilcek et al, 1994). The amplified 297-bp DNA fragment was purified using an Agarose Gel DNA Extraction Kit (INtRON, Daejeon, Korea) and subcloned into the vector pGEM-T (Promega, Madison, WI, USA) according to the manufacturer's instructions.…”

Section: Elisa and Rt-pcrmentioning

confidence: 99%

Serological and virological investigation of pestiviruses in Korean black goats

Oem¹,

Eunyong²,

Byun³

et al. 2012

Korean Journal of Veterinary Service

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Blood samples were collected from 672 goats in 60 farms from five provinces of Korea between November 2009 and August 2011. The prevalence of antibodies to pestiviruses was investigated. The examination for antibodies was performed using an enzyme-linked immunosorbent assay (ELISA) detecting antibodies to the bovine viral diarrhea virus (BVDV) and border disease virus (BDV). All blood samples were screened using reverse transcription-polymerase chain reaction (RT-PCR) with primer pairs specific to common pestivirus genome regions. The observed individual seroprevalence was 1.49% and herd seroprevalence was 11.67%. Also, the specific genomes to pestiviruses were detected in 3 out of the 915 clinical samples (0.45%). Based on the nucleotide sequence data, detected pestiviruses were belonged to two BVDV type-1 and one BVDV type-2. The pestivirus infection has been occurred among Korean black goats. However, our results indicate that the prevalence of pestiviruses in black goats was not significantly higher on farms with cattle.

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Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis

Cited by 569 publications

References 43 publications

Natural infection of cattle with an atypical `HoBi'-like pestivirus – Implications for BVD control and for the safety of biological products

Natural infection of cattle with an atypical `HoBi'-like pestivirus – Implications for BVD control and for the safety of biological products

BVDV control program in Austria - is a monitoring of the BDV status in sheep in Austria necessary?

Serological and virological investigation of pestiviruses in Korean black goats

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