Petroleum hydrocarbon bioremediation: sampling and analytical techniques, in situ treatments and commercial microorganisms currently used

Korda, Anna; Santas, Ph.; Tenente, A.; Santas, Regas

doi:10.1007/s002530051115

Cited by 141 publications

(90 citation statements)

References 7 publications

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“…In the case of in situ subsurface bioremediation processes, the greatest challenges relate to engineering of the subsurface environment so that microbes can thrive there and effectively degrade the contaminants present. Biological methods for processing of oily sludges and oil-contaminated soils in landfarming, biopiling/composting, bioventing, and bioreactor configurations have been well documented (30,342,489,652).…”

Section: Treatment Of Contaminated Soils and Sludgesmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,201

640

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Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments

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Section: Treatment Of Contaminated Soils and Sludgesmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,201

640

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“…exist for the remediation of such polluted environments, the use of the latter is continuously 53" increasing due to their much lower costs and environmental friendly nature [3][4][5].…”

Section: "mentioning

confidence: 99%

Allochthonous bioaugmentation in ex situ treatment of crude oil-polluted sediments in the presence of an effective degrading indigenous microbiome

Fodelianakis

Antoniou

Mapelli

et al. 2015

Journal of Hazardous Materials

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28"Oil-polluted sediment bioremediation depends on both physicochemical and 29" biological parameters, but the effect of the latter cannot be evaluated without the optimization 30" of the former. We aimed in optimizing the physicochemical parameters related to 31" biodegradation by applying an ex-situ landfarming set-up combined with biostimulation to 32" oil-polluted sediment, in order to determine the added effect of bioaugmentation by four 33" allochthonous oil-degrading bacterial consortia in relation to the degradation efficiency of the 34" indigenous community. We monitored hydrocarbon degradation, sediment ecotoxicity and 35"hydrolytic activity, bacterial population sizes and bacterial community dynamics, 36"characterizing the dominant taxa through time and at each treatment. We observed no 37" significant differences in total degradation, but increased ecotoxicity between the different 38" treatments receiving both biostimulation and bioaugmentation and the biostimulated-only 39" control. Moreover, the added allochthonous bacteria quickly perished and were rarely 40" detected, their addition inducing minimal shifts in community structure although it altered the 41" distribution of the residual hydrocarbons in two treatments. Therefore, we concluded that 42" biodegradation was mostly performed by the autochthonous populations while 43" bioaugmentation, in contrast to biostimulation, did not enhance the remediation process. Our 44" results indicate that when environmental conditions are optimized, the indigenous 45" microbiome at a polluted site will likely outperform any allochthonous consortium. 46"47"

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“…Biodegradation of hydrocarbons by natural populations of microorganisms is the main process acting in the depuration of hydrocarbon-polluted environments. The mechanism has been extensively studied and reviewed (Atlas, 1984;Leahy and Colwell, 1990;Korda et al, 1997;Kanaly and Harayama, 2000;Vam Hamme et al, 2003;Pinzon-Martinez et al, 2010).…”

Section: Introductionmentioning

confidence: 99%