PFGE characterisation and adhesion ability of Listeria monocytogenes isolates obtained from bovine carcasses and beef processing facilities

Galvão, Newton Nascentes; Chiarini, Eb; Destro, Maria Teresa; Ferreira, Márcia de Aguiar; Nero, Luís Augusto

doi:10.1016/j.meatsci.2012.06.011

Cited by 37 publications

(28 citation statements)

References 40 publications

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“…Essa técnica também pode permitir a identificação de pulsotipos específicos que não puderam ser diferenciados previamente pela sorotipificação (Borucki et al 2004). No Brasil há relatos da utilização desta técnica em cepas de Listeria monocytogenes detectadas em diferentes tipos de alimentos bem como em casos clínicos (Galvão et al 2012, Camargo 2013, Barbosa et al 2015, porém no Distrito Federal essa técnica em cepas de Listeria ainda é pouco usada, restringindo-se à cepas oriundas de casos clínicos.…”

Section: Caracterização Molecular Deunclassified

“…Galvão et al (2012) também identificaram potenciais vias de contaminação e permanência de cepas nos estabelecimentos por ele avaliado, em que um isolado observado em um estabelecimento de processamento foi obtido a partir do produto final (carne moída) e apresentou uma similaridade de 82% com outros seis outros isolados, todos obtidos no mesmo estabelecimento em datas anteriores realizados durante um período de 5 meses.…”

Section: Analises De Pfgeunclassified

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Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil

et al. 2016

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The aim of the study was the analysis of Listeria monocytogenes strains in beef samples as well as slaughterhouse environment, located in the Federal District, promote serotyping by polymerase chain reaction (PCR), perform antibiotic susceptibility and submit the strains to Pulsed-field gel electrophoresis (PFGE). A total of 125 beef samples were analyzed, 45 samples of carcasses swabs and 43 swab samples. It detected 13 strains of Listeria monocytogenes, 11 in beef samples. and 2 in slaughterhouse environment. No carcass swabs strains were isolated. Among the 13 strains of L. monocytogenes six strains of serotype 4b were found, five serotype 1/2c and two strains of serotype 1/2a. Among the 11 strains of L. monocytogenes found in beef, one (9.1%) strain showed resistance to erythromycin, one (9.1%) strain to gentamicin, one to ciprofloxacin (9.1%) and all strains (100%) were resistant to nalidixic acid. The two strains coming from the slaughterhouse drains, all (100%) were resistant to nalidixic acid and Sulfonamides. The analysis by pulsed field gel electrophoresis (PFGE) showed 13 different pulsotypes; they were grouped into three different clonal groups, coincidentally correlated with the three different serotypes found, what suggests a widespread dissemination of these profiles in the Federal District, Brazil.

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Section: Caracterização Molecular Deunclassified

Section: Analises De Pfgeunclassified

Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil

et al. 2016

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“…monocytogenes is a pathogenic bacterium largely disseminated in the environment and has been isolated in soil, water, sewage, vegetables, silages, healthy human and animal feces [20]. Other authors have also detected L. monocytogenes in broiler carcasses during the meat processing stages [21,22,23].…”

Section: Discussionmentioning

confidence: 99%

Total and Thermo-Tolerant Coliforms, Salmonella spp., Escherichia coli, Pseudomonas spp and Listeria monocytogenes in Broilers Chicken Meat Processing Chain in Southern

Pacheco¹,

Bairros²,

Passos³

et al. 2017

JFNR

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Sixty-six samples were collected from a broiler chicken abattoir. Eighteen samples were collected from the retail market in Southern Brazil, where the slaughtered broilers were commercialized, so that the microbiological assessment of the samples in the broiler industrial chain could be undertaken. And eighteen samples submitted to clients´ common home procedures were similarly assessed. All samples were evaluated by quantifying total and thermotolerant coliforms, psycrotrophic bacteria, Pseudomonas spp, Escherichia coli and research for Salmonella spp and Listeria monocytogenes. Results on counts and on the occurrence of microorganisms in samples from specific sites in the broiler processing chain demonstrate the need for a re-assessment of hygiene practices throughout the whole process and educational activities should be direct to all people involved, including consumers.

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“…In Brazil, there have not been any documented outbreaks of listeriosis associated with the consumption of contaminated food. However, L. monocytogenes has been successfully isolated from different food products (NALERIO et al, 2009;BUENO et al, 2010;GALVÃO et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Genotypic profile of Listeria monocytogenes isolated in refrigerated chickens in southern Rio Grande do Sul, Brazil

et al. 2015

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Listeria monocytogenes is of notable concern to the food industry, due to its ubiquitous nature and ability to grow in adverse conditions. This study aimed to determine the genotypic profile of L. monocytogenes strains isolated from refrigerated chickens marketed in the southern part of Rio Grande do Sul, Brazil. The strains of L. monocytogenes isolated were characterized by serotyping and Pulsed Field Gel Electrophoresis (PFGE). Three different serotypes (1/2a, 1/2b and 4e) were evaluated by PFGE, and the macrorestriction patterns utilizing enzymes AscI and ApaI, revealed five different pulsotypes. The presence of such varied genotypic profiles demonstrates the prevalence of L. monocytogenes contamination of chicken processing environments, which combined with ineffective cleaning procedures, allowing the survival, adaptation and proliferation of these pathogens, not only in the processing environment, but also in local grocery stores.

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PFGE characterisation and adhesion ability of Listeria monocytogenes isolates obtained from bovine carcasses and beef processing facilities

Cited by 37 publications

References 40 publications

Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil

Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil

Total and Thermo-Tolerant Coliforms<i>,</i><i> </i><i>Salmonella</i><i> </i><i>spp.,</i><i> </i><i>Escherichia</i><i> </i><i>coli,</i><i> </i><i>Pseudomonas</i><i> </i><i>spp</i><i> </i>and <i>Listeria</i><i> </i><i>monocytogenes</i><i> </i>in Broilers Chicken Meat Processing Chain in Southern

Genotypic profile of Listeria monocytogenes isolated in refrigerated chickens in southern Rio Grande do Sul, Brazil

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