pH Effects on solubility, zeta potential, and correlation between antibacterial activity and molecular weight of chitosan

Chang, Shun-Hsien; Lin, Hong‐Ting Victor; Wu, Guan-James; Tsai, Guo Jane

doi:10.1016/j.carbpol.2015.07.072

Cited by 298 publications

(129 citation statements)

References 37 publications

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“…It is evident that with the increasing pH value, from 8.5 to 12.5, a predominant alteration in the zeta potential in descending order (39.30 mV to 5.22 mV), indicate a large effect on the stability of the particles in colloidal dispersion, based on size and charge . The cationic CaAl‐LDH nanoparticles precipitated at pH 8.5, experiences an enhanced concentration of the [OH] − ions in the dispersion medium at higher pH values (10.5‐12.5), approaching toward the attainment of the isoelectric point (IEP) for the system slowly and hence, the environment of the maximum instability, leading to the particle coagulation and flocculation, tending to formation of bigger particles, having size in the range 400‐500 nm.…”

Section: Discussionmentioning

confidence: 99%

pH‐dependent facile synthesis of CaAl‐layered double hydroxides and its effect on the growth inhibition of cancer cells

et al. 2018

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The present communication reports a pH‐dependent facile synthesis of calcium aluminum layered double hydroxide (CaAl‐LDH), at 3 different pH conditions of 8.5, 10.5 and 12.5 respectively, by simple coprecipitation route that led to the precipitation of the same in phase pure or in mixed phase form, and their detail characterization. Interestingly, at a pH of 8.5, the precipitate obtained was 100% phase pure CaAl‐LDH, represented as sample A, whereas, at higher pH conditions, a mixed phase comprising 37% of a combination of CaCO3 polymorphs, e.g., calcite and aragonite and 63% of the CaAl‐LDH (at pH 10.5, represented as sample B) and 43% of a combination of calcite and aragonite and the rest 57% being CaAl‐LDH (at pH 12.5, represented as sample C) respectively, were obtained. The increasing trend of the presence of carbonate anion (with reference to calcite and aragonite phases) was estimated by trace level ion‐exchange chromatography study using organic acid column. All the above samples were tested for their anticancer activity in vitro, using cancer cell lines, e.g., human colon carcinoma (HCT116) and human breast adenocarcinoma (MCF7). Interestingly, sample A only exhibited significant growth inhibition of the cancer cells as above‐mentioned. The observation as above was verified using human osteoblast precursor cells (MC3T3) as well, on which, all the samples (sample A, sample B and sample C) exhibited good cellular viability.

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Section: Discussionmentioning

confidence: 99%

pH‐dependent facile synthesis of CaAl‐layered double hydroxides and its effect on the growth inhibition of cancer cells

et al. 2018

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“…This may partly be explained by differences in the cell wall and expression of different surface molecules, in addition to differences in size, molecular weight, and deacetylation of chitosan itself [28]. In this study, the LVC used had a MW of 150 kDa and chitosan of this size has shown good solubility (9.98 mg/mL) in phosphate buffer and good antibacterial effect at pH 6 [29]. The antibacterial activity of dissolved chitosan is dependent on the pH of the solution, and the antibacterial effect is reduced at higher pH due to less positive charged amino groups [30].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Antibiofilm Effect of Low Viscosity Chitosan againstStaphylococcus epidermidis

Dragland

Valen

Stenhagen

et al. 2016

International Journal of Microbiology

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Aim. The aim of this study was to investigate the antibacterial and antibiofilm properties of low viscosity chitosan on S. epidermidis growth and biofilm formation. Methods and Results. The antibacterial and antibiofilm properties were investigated, during both planktonic growth and biofilm formation. This was performed using different concentrations in media and by coating on polystyrene surfaces. In addition, the bactericidal effect was investigated using a modified direct contact test. The results showed that low viscosity chitosan in media had both a bacteriostatic and bactericidal effect on planktonic growth and biofilm formation of S. epidermidis in a concentration dependent manner. Polystyrene discs coated with chitosan reduced both early biofilm formation (6 h) and late biofilm formation (18 h), as confirmed by scanning electron microscopy. The modified direct contact test showed a bactericidal effect. Conclusion. This study demonstrated that low viscosity chitosan has a bacteriostatic and bactericidal activity against S. epidermidis and that the activity is dependent on the amount of chitosan added. In addition, low viscosity chitosan reduced biofilm formation both when added to media and when coated on polystyrene surfaces. Significance and Impact of Study. Low viscosity chitosan could be a contribution to new treatment approaches of biofilm-related infections of S. epidermidis.

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“…[8] CH coating has been shown to significantly improve the texture parameters and sensory scores of shrimp compared with a control sample. [9] The antimicrobial properties of CH have been reported by Genskowsky et al [10] that films containing only CH were effective against some Gram-positive bacteria.…”

mentioning

confidence: 99%

Protein denaturation and oxidation in chilled hairtail (Trichiutus haumela) as affected by electrolyzed oxidizing water and chitosan treatment

Luan

Wang

et al. 2017

International Journal of Food Properties

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The effect of chitosan (CH) coating combined with electrolyzed oxidizing water (EOW) on microbiological and physicochemical of hairtail (Trichiutus haumela) was evaluated during 15 days of storage at −3°C. Hairtail samples treated by EOW combined with 1.5% (w/v) CH were compared with samples soaked in single material. For storage of 15 days, the thiobarbituric acid value of hairtails increased steadily to 1.79, 1.52, 1.48 mg/100g (p < 0.05) in the EOW, CH, and EOW+CH group, respectively. All of these were significantly lower than that of the control group (2.01 ± 0.04 mg malonaldehyde/ 100g; p < 0.05). Microbiological results, total volatile basic nitrogen, pH, and peroxide value also revealed that EOW and CH significantly retarded hairtail spoilage. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis pattern of sarcoplasmic proteins revealed CH+EOW could slow down the protein deterioration of fillet. In this study, CH coating combined with EOW effectively extended the shelf life of hairtail to 6-7 days. ARTICLE HISTORY

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pH Effects on solubility, zeta potential, and correlation between antibacterial activity and molecular weight of chitosan

Cited by 298 publications

References 37 publications

pH‐dependent facile synthesis of CaAl‐layered double hydroxides and its effect on the growth inhibition of cancer cells

pH‐dependent facile synthesis of CaAl‐layered double hydroxides and its effect on the growth inhibition of cancer cells

Antibacterial and Antibiofilm Effect of Low Viscosity Chitosan againstStaphylococcus epidermidis

Protein denaturation and oxidation in chilled hairtail (Trichiutus haumela) as affected by electrolyzed oxidizing water and chitosan treatment

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