pH-responsive DNA hydrogels with ratiometric fluorescence for accurate detection of miRNA-21

Lai

et al. 2023

Biosensors

Food safety has always been a major global challenge to human health and the effective detection of harmful substances in food can reduce the risk to human health. However, the food industry has been plagued by a lack of effective and sensitive safety monitoring methods due to the tension between the cost and effectiveness of monitoring. DNA-based hydrogels combine the advantages of biocompatibility, programmability, the molecular recognition of DNA molecules, and the hydrophilicity of hydrogels, making them a hotspot in the research field of new nanomaterials. The stimulus response property greatly broadens the function and application range of DNA hydrogel. In recent years, DNA hydrogels based on stimulus-responsive mechanisms have been widely applied in the field of biosensing for the detection of a variety of target substances, including various food contaminants. In this review, we describe the recent advances in the preparation of stimuli-responsive DNA hydrogels, highlighting the progress of its application in food safety detection. Finally, we also discuss the challenges and future application of stimulus-responsive DNA hydrogels.

Section: Stimulus-responsive Dna Hydrogel-based Biosensormentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Lai

et al. 2023

Biosensors

“…Figure C shows the pH responsiveness of the hydrogels in pH 5.0 and 8.0 buffers. Yao et al designed a pH-responsive DNA hydrogel with fluorescence ratios to enhance the accuracy of DNA hydrogel detection. Jeong et al developed a capillary assay method based on a target DNA and a pH responsive DNA hydrogel.…”

Section: Stimulus-responsive Dna Hydrogelsmentioning

confidence: 99%

“…99 Figure 4C shows the pH responsiveness of the hydrogels in pH 5.0 and 8.0 buffers. Yao et al 100 95 Copyright 2022, American Chemical Society. (B) Cyclic transitions of a nucleic acid (1) + (2)-functionalized acrylamide copolymer by pH stimuli.…”

Section: Stimulus-responsive Dna Hydrogelsmentioning

confidence: 99%

Historical Perspectives and Recent Research on DNA Hydrogel-Based Stimuli-Responsive Systems and Bioengineering Applications

Cao

ACS Appl. Polym. Mater.

et al. 2022

Soft hydrogel materials have attracted increasing attention from scientists due to the programmability of nucleic acids. DNA hydrogels are increasingly used in biomedicine due to their natural biocompatibility and degradation properties. Moreover, the addition of various molecules to these hydrogels can enhance the superiority and utility of DNA hydrogels. Therefore, the present study aimed to provide a timely overview of historical perspectives and recent research advances in DNA hydrogels and their applications in bioengineering and stimulus-responsive systems. The state-of-the-art problems and future prospects of DNA hydrogels were also discussed.

Stimuli‐Responsive Self‐Degradable DNA Hydrogels: Design, Synthesis, and Applications

Adv Healthcare Materials

Duan

Liu

et al. 2023

DNA hydrogels play an increasingly important role in biomedicine and bioanalysis applications. Due to their high programmability, multifunctionality and biocompatibility, they are often used as effective carriers for packing drugs, cells, or other bioactive cargoes in vitro and in vivo. However, the stability of the DNA hydrogels prevents their in‐demand rapid release of cargoes to achieve a full therapeutic effect in time. For bioanalysis, the generation of signals sometimes needs the DNA hydrogel to be rapidly degraded when sensing target molecules. To meet these requirements, stimulus‐responsive DNA hydrogels are designed. By responding to different stimuli, self‐degradable DNA hydrogels can switch from gel to solution for quantitative bioanalysis and precision cargo delivery. This review summarizes the recently developed innovative methods for designing stimuli‐responsive self‐degradable DNA hydrogels and showed their applications in the bioanalysis and biomedicines fields. Challenges, as well as prospects, are also discussed.