pH-responsive zwitterionic copolypeptides as charge conversional shielding system for gene carriers

Tian, Huayu; Guo, Zhaopei; Lin, Lin; Zhang, Jiao; Chen, Jie; Gao, Shiqian; Zhu, Xuemei; Chen, Xuesi

doi:10.1016/j.jconrel.2013.11.008

Cited by 99 publications

(77 citation statements)

References 31 publications

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“…In each cell line, the TE of Ga-PEI was higher than Ser-PEI, which also has hydroxyl groups but maintains the amount of amino groups. This indicates that the balance between electron-positive/negative groups, which also called the "hydroxyl effect" [11,28] or the charge screening effect [29,30], is important for the transfection by such types of polycations, especially in the serum circumstance. On the other hand, Gly-PEI also showed good TE, but the relative TE compared to PEI was lower than that obtained without serum.…”

Section: In Vitro Gene Transfectionmentioning

confidence: 99%

Amino Acid-Modified Polyethylenimines with Enhanced Gene Delivery Efficiency and Biocompatibility

et al. 2015

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Abstract:The development of gene delivery vectors with high efficiency and biocompatibility is one of the key points of gene therapy. A series of polycations were prepared from polyethylenimine (PEI) with several amino acids or their analogs. The target polymers have different charge and hydrophilic/hydrophobic properties, which may affect their performance in the gene transfection process. Gel retardation and DLS assays showed that these polymers may condense DNA into nanoparticles with positive zeta potentials and proper sizes for cellular uptake. Luciferase reporter gene transfection results revealed their higher transfection efficiency than PEI; especially in the presence of serum, in which up to 23 times higher efficiency was achieved by employing glycolic acid-grafted PEI. Moreover, it was found that the degree of substitution on PEI has an apparent influence on the transfection, and the balance between electron-positive/negative groups largely affects the delivery process. The higher serum tolerance was also proven by BSA adsorption, flow cytometry and confocal microscopy assays. Results demonstrate that such type of polycations may serve as promising non-viral gene delivery vectors.

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Section: In Vitro Gene Transfectionmentioning

confidence: 99%

Amino Acid-Modified Polyethylenimines with Enhanced Gene Delivery Efficiency and Biocompatibility

et al. 2015

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“…12,40 In this study, the biodegradable material HA with a negative charge was chosen as a shell to shield the positive charge of DDN. The formation of both DDN and HDDN was through electrostatic interaction.…”

Section: Preparation and Characterization Of Hddnmentioning

confidence: 99%

Tumor microenvironment dual-responsive core–shell nanoparticles with hyaluronic acid-shield for efficient co-delivery of doxorubicin and plasmid DNA

Wang¹,

Yu²,

Han³

et al. 2017

IJN

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As the tumor microenvironment (TME) develops, it is critical to take the alterations of pH value, reduction and various enzymes of the TME into consideration when constructing the desirable co-delivery systems. Herein, TME pH and enzyme dual-responsive core–shell nanoparticles were prepared for the efficient co-delivery of chemotherapy drug and plasmid DNA (pDNA). A novel pH-responsive, positively charged drug loading material, doxorubicin (DOX)-4-hydrazinobenzoic acid (HBA)-polyethyleneimine (PEI) conjugate (DOX-HBA-PEI, DHP), was synthesized to fabricate positively charged polyion complex inner core DHP/DNA nanoparticles (DDN). Hyaluronic acid (HA) was an enzyme-responsive shell which could protect the core and enhance the co-delivery efficiency through CD44-mediated endocytosis. The HA-shielded pH and enzyme dual-responsive nanoparticles (HDDN) were spherical with narrow distribution. The particle size of HDDN was 148.3±3.88 nm and the zeta potential was changed to negative (−18.1±2.03 mV), which led to decreased cytotoxicity. The cumulative release of DOX from DHP at pH 5.0 (66.4%) was higher than that at pH 7.4 (30.1%), which indicated the pH sensitivity of DHP. The transfection efficiency of HDDN in 10% serum was equal to that in the absence of serum, while the transfection of DDN was significantly decreased in the presence of 10% serum. Furthermore, cellular uptake studies and co-localization assay showed that HDDN were internalized effectively through CD44-mediated endocytosis in the tumor cells. The efficient co-delivery of DOX and pEGFP was confirmed by fluorescent image taken by laser confocal microscope. It can be concluded that TME dual-responsive HA-shielded core–shell nanoparticles could be considered as a promising platform for the co-delivery of chemotherapy drug and pDNA.

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“…Stimuli-responsive polymers have received extensive attention in biotechnological and biomedical applications, especially in drug delivery systems [1,2] and gene delivery carriers [3,4].…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Temperature- and pH Dually-responsive Poly(β-amino ester)

Lu¹,

Yang²,

Li³

et al. 2017

dtetr

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Stimuli-responsive polymers have undoubtedly been of great interest in the past few decades due to a variety of potential applications in biomedical territory but their non-degradability limits their in vivo applications. Herein, we report a novel dual-stimuli responsive pH-and temperature-responsive poly(β-amino ester). The pH/temperature sensitivities are interrelated and can be easily tuned by the PEG-diacrylate chain length and the percentage of biamines in the feed ratio. These dually-responsive polymers are very useful in drug delivery.

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pH-responsive zwitterionic copolypeptides as charge conversional shielding system for gene carriers

Cited by 99 publications

References 31 publications

Amino Acid-Modified Polyethylenimines with Enhanced Gene Delivery Efficiency and Biocompatibility

Amino Acid-Modified Polyethylenimines with Enhanced Gene Delivery Efficiency and Biocompatibility

Tumor microenvironment dual-responsive core–shell nanoparticles with hyaluronic acid-shield for efficient co-delivery of doxorubicin and plasmid DNA

Biodegradable Temperature- and pH Dually-responsive Poly(β-amino ester)

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pH-responsive zwitterionic copolypeptides as charge conversional shielding system for gene carriers

Cited by 99 publications

References 31 publications

Amino Acid-Modified Polyethylenimines with Enhanced Gene Delivery Efficiency and Biocompatibility

Amino Acid-Modified Polyethylenimines with Enhanced Gene Delivery Efficiency and Biocompatibility

Tumor microenvironment dual-responsive core&ndash;shell nanoparticles with hyaluronic acid-shield for efficient co-delivery of doxorubicin and plasmid DNA

Biodegradable Temperature- and pH Dually-responsive Poly(β-amino ester)

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Tumor microenvironment dual-responsive core–shell nanoparticles with hyaluronic acid-shield for efficient co-delivery of doxorubicin and plasmid DNA