pH-Sensitive fusogenic polymer-modified liposomes as a carrier of antigenic proteins for activation of cellular immunity

Yuba, Eiji; Kojima, Chie; Harada, Atsushi; Tana,; Watarai, Shinobu; Kono, Kenji

doi:10.1016/j.biomaterials.2009.10.006

Cited by 107 publications

(102 citation statements)

References 26 publications

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“…This result suggests that liposomes having polymers of a hyperbranched structure were taken up by cells more efficiently than those with the polymers of a linear structure. We have shown that linear MGluPG-modified liposomes are taken up by DC2.4 cells through their interaction with the cellular scavenger receptors, which recognize carboxylate anions of polymers [18,24]. Negatively charged carboxylate groups of the hyperbranched polymer tend to locate in the peripheral region of the polymer.…”

Section: Preparation Of Ph-sensitive Liposomes Using Hpgmentioning

confidence: 99%

Carboxylated hyperbranched poly(glycidol)s for preparation of pH-sensitive liposomes

Yuba

Harada

Sakanishi

et al. 2011

Journal of Controlled Release

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106

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Previous reports by the authors described intracellular delivery using liposomes modified with various carboxylated poly(glycidol) derivatives. These linear polymer-modified liposomes exhibited pH-dependent membrane fusion behavior in cellular acidic compartments. However, the effect of the backbone structure on membrane fusion activity remains unknown. Therefore, this study specifically investigated the backbone structure to obtain pH-sensitive polymers with much higher fusogenic activity and to reveal the effect of the polymer backbone structure on interaction with the membrane.Hyperbranched poly(glycidol) (HPG) derivatives were prepared as a new type of pH-sensitive polymers and modified liposomes using these polymers. The resultant HPG derivatives exhibited high hydrophobicity and intensive interaction with the membrane concomitantly with the increasing degree of polymerization. Furthermore, HPG derivatives showed stronger interaction with the membrane than linear polymers show. Liposomes modified with HPG derivatives of high DP delivered contents into cytosol of DC2.4 cells, a dendritic cell line, more effectively than the linear polymer-modified liposomes do. Results show that the backbone structure of pH-sensitive polymers affected their pH-sensitivity and interaction with liposomal and cellular membranes.

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Section: Preparation Of Ph-sensitive Liposomes Using Hpgmentioning

confidence: 99%

Carboxylated hyperbranched poly(glycidol)s for preparation of pH-sensitive liposomes

Yuba

Harada

Sakanishi

et al. 2011

Journal of Controlled Release

Self Cite

106

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“…Carrier for antigenic protein: vaccines and nanosensors for neglected diseases Efficient vaccination strategies have been desired for overcoming new pathogens and evolution of resistance by microorganisms (Yuba et al 2010). However, effective vaccines to eliminate pathogens entrenched in the human population, such as malaria, leishmaniasis, measles, acute respiratory infections, and HIV, are still to be developed (Kalkanidis et al 2006;Nagill and Kaur 2010).…”

Section: New Perspectives For Applications Of Proteoliposomesmentioning

confidence: 99%

Proteoliposomes in nanobiotechnology

et al. 2012

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Proteoliposomes are systems that mimic lipid membranes (liposomes) to which a protein has been incorporated or inserted. During the last decade, these systems have gained prominence as tools for biophysical studies on lipid-protein interactions as well as for their biotechnological applications. Proteoliposomes have a major advantage when compared with natural membrane systems, since they can be obtained with a smaller number of lipidic (and protein) components, facilitating the design and interpretation of certain experiments. However, they have the disadvantage of requiring methodological standardization for incorporation of each specific protein, and the need to verify that the reconstitution procedure has yielded the correct orientation of the protein in the proteoliposome system with recovery of its functional activity. In this review, we chose two proteins under study in our laboratory to exemplify the steps necessary for the standardization of the reconstitution of membrane proteins in liposome systems: (1) alkaline phosphatase, a protein with a glycosylphosphatidylinositol anchor, and (2) Na,K-ATPase, an integral membrane protein. In these examples, we focus on the production of the specific proteoliposomes, as well as on their biochemical and biophysical characterization, with emphasis on studies of lipid-protein interactions. We conclude the chapter by highlighting current prospects of this technology for biotechnological applications, including the construction of nanosensors and of a multiprotein nanovesicular biomimetic to study the processes of initiation of skeletal mineralization.

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“…These polymermodified liposomes delivered calcein into cytosol of HeLa cells after internalization via endocytosis and subsequent fusion with membrane of endosome or lysosome (Sakaguchi et al, 2008b). In this sequence Yuba et al (2010) reported pH-sensitive fusogenic liposomes modified with carboxylated poly(glycidol) derivatives, such as polymer-succinylated poly(glycidol) (SucPG) and 3-methylglutarylated poly(glycidol) (MGluPG) that can deliver antigenic proteins ovalbumin (OVA) into the cytosol of DCs. The nasal administration of these liposomes activated the antigen-specific cellular immunity.…”

Section: The Ph-sensitive Liposomes In Vaccine Deliverymentioning

confidence: 99%

A review of mechanistic insight and application of pH-sensitive liposomes in drug delivery

2014

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The pH-sensitive liposomes have been extensively used as an alternative to conventional liposomes in effective intracellular delivery of therapeutics/antigen/DNA/diagnostics to various compartments of the target cell. Such liposomes are destabilized under acidic conditions of the endocytotic pathway as they usually contain pH-sensitive lipid components. Therefore, the encapsulated content is delivered into the intracellular bio-environment through destabilization or its fusion with the endosomal membrane. The therapeutic efficacy of pHsensitive liposomes enables them as biomaterial with commercial utility especially in cancer treatment. In addition, targeting ligands including antibodies can be anchored on the surface of pH-sensitive liposomes to target specific cell surface receptors/antigen present on tumor cells. These vesicles have also been widely explored for antigen delivery and serve as immunological adjuvant to enhance the immune response to antigens. The present review deals with recent research updates on application of pH-sensitive liposomes in chemotherapy/ diagnostics/antigen/gene delivery etc.

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pH-Sensitive fusogenic polymer-modified liposomes as a carrier of antigenic proteins for activation of cellular immunity

Cited by 107 publications

References 26 publications

Carboxylated hyperbranched poly(glycidol)s for preparation of pH-sensitive liposomes

Carboxylated hyperbranched poly(glycidol)s for preparation of pH-sensitive liposomes

Proteoliposomes in nanobiotechnology

A review of mechanistic insight and application of pH-sensitive liposomes in drug delivery

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