PHA-L lectin and carbohydrate relationship: conjugation with CdSe/CdS nanoparticles, radiolabeling and in vitro affinities on MCF-7 cells

Kara, Altan; Ünak, Perihan; Selçuki, Cenk; Akca, Ozlet; Medine, E. Ilker; Sakarya, Serhan

doi:10.1007/s10967-013-2783-5

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…It has been reported as chemo-sensitization and radioprotection agent and investigated in various tumor cell lines and chemical induced carcinogenesis [24]. In our another work, the inter- actions between PHA-Lec (phytohemagglutinin-L) surface and sialic acid were calculated by computational tools [22]. These calculations revealed specific interactions between PHA-L and sialic acid.…”

Section: Radioiodinationmentioning

confidence: 94%

“…MTC-QD and MTC-QDs-Lec were labeled with 125 I according to the nucleophilic substitution [22]. Although mannose triflate is a fluorination agent, it can be also used for the nucleophilic iodination.…”

Section: Labeling With 125 Imentioning

confidence: 99%

“…It has been used to label various biomolecules [14][15][16][17][18] such as proteins, peptides, and antibodies [19,20]. Regarding with Lec, V. Album agglutinin [21], phytohemagglutinin-L [22], and Viscum album agglutinin-I Lec [21] were labeled with 125 I via iodogen method to show receptor ligand interactions. Recently, QD nanoparticles were also labeled with some radionuclides and used as dual imaging probes for the targeted therapy [23].…”

Section: Radioiodinationmentioning

confidence: 99%

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Radioiodine labeled CdSe/CdS quantum dots: Lectin targeted dual probes

et al. 2014

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CdSe/CdS quantum dots (QD) were synthesized and bioconjugated with Sambucus nigra agglutinin (SNA) lectin (Lec). Mannose triflate and cysteamine molecules (MTC) were also utilized to prepare MTC-QDs and MTCQDs-Lec probes as well as Lec bound QDs. Afterwards; potential use of these nanoparticles as radiolabeled fluorescence nano-probes for the cell imaging studies has been investigated. Biological activities of 125 I − , 125 I-MTCQDs, MTC-QDs-Lec-125 I, QDs-Lec-125 I and Lec-125 I were examined on various cancer cell lines such as Caco-2, MCF-7 and A-549 in terms of cell incorporation. QDs-Lec-125 I exhibited the highest cell incorporation on whole celllines. In addition, the QDs-Lec-131 I, was used for in vivo imaging. The whole body distribution of the radiolabeled QDs on New Zealand rabbits and Balb C mice were examined by taking dynamic and static images. Radioactivity cleared from the kidneys and the bladder, while significant amount radioactivity was retained in the heart and liver within 24 h.

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Section: Radioiodinationmentioning

confidence: 94%

Section: Labeling With 125 Imentioning

confidence: 99%

Section: Radioiodinationmentioning

confidence: 99%

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Radioiodine labeled CdSe/CdS quantum dots: Lectin targeted dual probes

et al. 2014

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“…In addition to lactose, which enhanced the uptake of the QD‐glycan NPs into cancer cells, the composition of a mixed carbohydrate shell (i.e., lactose and mannose vs. lactose and maltotriose) significantly altered the internalization of NPs . In another study, a secondary radiotracer ( 125 I) was used as the probe conjugated with CdSe/CdS QDs decorated with a sialic acid‐binding lectin . These NPs were able to image breast cancer cells, as revealed by in vitro experiments .…”

Section: Cell/tissue Targetingmentioning

confidence: 98%

Nanotechnology in Glycomics: Applications in Diagnostics, Therapy, Imaging, and Separation Processes

Dosekova

Filip

Bertók

et al. 2016

Medicinal Research Reviews

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This review comprehensively covers the most recent achievements (from 2013) in the successful integration of nanomaterials in the field of glycomics. The first part of the paper addresses the beneficial properties of nanomaterials for the construction of biosensors, bioanalytical devices, and protocols for the detection of various analytes, including viruses and whole cells, together with their key characteristics. The second part of the review focuses on the application of nanomaterials integrated with glycans for various biomedical applications, that is, vaccines against viral and bacterial infections and cancer cells, as therapeutic agents, for in vivo imaging and nuclear magnetic resonance imaging, and for selective drug delivery. The final part of the review describes various ways in which glycan enrichment can be effectively done using nanomaterials, molecularly imprinted polymers with polymer thickness controlled at the nanoscale, with a subsequent analysis of glycans by mass spectrometry. A short section describing an active glycoprofiling by microengines (microrockets) is covered as well.

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“…Kara et al [53] prepared CdSe/CdS QDs, conjugated with PHA-L and labelled with 125 I to study the interface between phytohemagglutinin-L (PHA-L) and sialic acid. Sialic acid is abundant on the breast cancer cell (MCF-7) surface.…”

Section: Lectin Functionalised Qds In Theranostics For Cancermentioning

confidence: 99%

Glyco-functionalised quantum dots and their progress in cancer diagnosis and treatment

Ashree

Wang

Chao

2019

Front. Chem. Sci. Eng.

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Despite all major breakthroughs in recent years of research, we are still unsuccessful to effectively diagnose and treat cancer that has express and metastasizes. Thus, the development of a novel approach for cancer detection and treatment is crucial. Recent progress in Glyconanotechnology has allowed the use of glycans and lectins as bio-functional molecules for many biological and biomedical applications. With the known advantages of quantum dots (QDs) and versatility of carbohydrates and lectins, Glyco-functionalised QD is a new prospect in constructing biomedical imaging platform for cancer behaviour study as well as treatment. In this review, we aim to describe the current utilisation of Glyco-functionalised QDs as well as their future prospective to interpret and confront cancer.

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PHA-L lectin and carbohydrate relationship: conjugation with CdSe/CdS nanoparticles, radiolabeling and in vitro affinities on MCF-7 cells

Cited by 7 publications

References 16 publications

Radioiodine labeled CdSe/CdS quantum dots: Lectin targeted dual probes

Radioiodine labeled CdSe/CdS quantum dots: Lectin targeted dual probes

Nanotechnology in Glycomics: Applications in Diagnostics, Therapy, Imaging, and Separation Processes

Glyco-functionalised quantum dots and their progress in cancer diagnosis and treatment

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