PHA production from the organic fraction of municipal solid waste (OFMSW): Overcoming the inhibitory matrix

Challenges of scaling-up PHA production from waste streams. A review

“…Urban waste treatment plant wastes include carbon sources from both municipal solid and wastewater treatment plants: activated wastewater sludge (Cha et al, 2016;Morgan-Sagastume et al, 2015;Jia et al, 2014), the organic fraction of municipal solid waste leachate (Korkakaki et al, 2016), activated wastewater sludge combined with the organic fraction of municipal solid waste (Basset et al, 2016).…”

“…This treatment is previous to PHA production, so it is called the pre-treatment phase. This phase might be used to increase the carbon sources available (Pittmann and Steinmetz, 2016;Haas et al, 2015;Oh et al, 2015;Kourmentza et al, 2015;Campanari et al, 2014;Ntaikou et al, 2014;Tamis et al, 2014;Valentino et al, 2015b;Pais et al, 2016;Colombo et al, 2016;Amulya et al, 2015;Bera et al, 2015;Pan et al, 2016;Morgan-Sagastume et al, 2015;Jia et al, 2014;Korkakaki et al, 2016;Basset et al, 2016;Chen et al, 2015b;Chen et al, 2016b), dilute the concentration of organic matter (Pittmann and Steinmetz, 2016;Bhati and Mallick, 2016;Alsafadi and Al-Mashaqbeh, 2017;Ntaikou et al, 2014;Amulya et al, 2015;Ray et al, 2016;Jia et al, 2014), regulate the pH (Patel et al, 2015;Kourmentza et al, 2015;Amulya et al, 2015), control the temperature (Kourmentza et al, 2015;Campanari et al, 2014), sterilize waste material (Oh et al, 2015;Kourmentza et al, 2015;Colombo et al, 2016), and/or remove suspended solids (Oh et al, 2015;Haas et al, 2015;Patel et al, 2015;Kourmentza et al, 2015;Campanari et al, 2014;Ntaikou et al, 2014;Valentino et al, 2015a;Amulya et al, ...…”

“…In order to increment the available carbon source, authors have used mostly two (Kourmentza et al, 2015;Campanari et al, 2014;Ntaikou et al, 2014), leachate from urban solid waste (Korkakaki et al, 2016). On the one hand, a clear relation between the waste material and the selected hydrolysis technique was not observed.…”

“…Mixed cultures for PHA production have been proposed due to the fact that they are simpler and less costly than the process with pure cultures, since sterile conditions and infrastructure for an axenic bioprocess are not required (Valentino et al, 2017). Indeed among the different mixed cultures reported in the Literature for the 2014-2016 period, the employment of wastewater activated sludge (WAS) was the most widespread (Tables 2 and 3) (Pittmann and Steinmetz, 2016;Cha et al, 2016;Korkakaki et al, 2016;Palmeiro-Sánchez et al, 2016;Amulya et al, 2016;Chen et al, 2015b;Chen et al, 2016a). WAS obtained from wastewater treatment plants could not be employed directly as culture for PHA production.…”

Survival of the fastest: Selective removal of the side population for enhanced PHA production in a mixed substrate enrichment

“…Nonetheless, when using real wastewater the experimental results indicated that PHA production is primarily associated with the VFA fraction of the wastewater (Coats et al, 2007;Bengtsson et al, 2008;Beccari et al, 2009;Albuquerque et al, 2010;Morgan-Sagastume et al, 2010;Jiang et al, 2012;Tamis et al, 2014a;Morgan-Sagastume et al, 2014, 2015Korkakaki et al, 2016). This correlation is based on the fact that the VFA carbon fraction serves as a PHA precursor and efficiently enriches for a PHA accumulating community.…”