Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals

Cabezas, Sheila; Rojas, Gertrudis; Pavón, Alequis; Bernardo, Lídice; Castellanos, Yinet; Álvarez, Mayling; Pupo, Maritza; Guillén, Gerardo; Guzmán, María G.

doi:10.1007/s00705-009-0401-1

Cited by 8 publications

(5 citation statements)

References 32 publications

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“…Phage antibody libraries have been widely used to select antibodies against infectious agents. These include antibodies that discriminate between different strains of Hantavirus71, Dengue virus72, influenza73,74, Ebola75 and Venezuelan equine encephalitis virus76. Given that many of these viruses are classified serologically, the ability to select phage antibodies with similar specificities is not surprising, but unlike antibodies generated by immunization, these have the potential to be used therapeutically.…”

Section: Specificity For Protein Sequences and Conformationsmentioning

confidence: 99%

Beyond natural antibodies: the power of in vitro display technologies

et al. 2011

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In vitro display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some twenty years ago. Since that time a large number of antibodies, some with remarkable properties, have been selected and improved upon using these methods. The first antibodies derived using in vitro display methods are now in the clinic, with many more waiting in the wings. Here we discuss the scope of the technology, some of the powerful antibodies selected, and the future potential in a post-genomic world. Unique advantages offered by in vitro display technologies include the ability to carefully define selection conditions, allowing the derivation of antibodies recognizing predefined epitopes or conformations, the further improvement of selected antibodies, the potential for high throughput applications and the immediate availability of genes encoding the selected antibody. We anticipate that the high throughput potential of these technologies will soon lead to their use to select antibodies against all human proteins.

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Section: Specificity For Protein Sequences and Conformationsmentioning

confidence: 99%

Beyond natural antibodies: the power of in vitro display technologies

et al. 2011

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“…Using an anti-PVIII antibody to detect bound phages interacting with an immobilized partner is thus a very effective amplification system to detect weak interactions. Such potential has been exploited by using whole phages as detector particles in diagnosis (Cabezas et al, 2009). The current work, aimed at detecting the moderate affinity interaction of IL-2 with the isolated alpha chain, as well as the even weaker residual binding of the mutated IL-2 variants, took advantage of this extremely high sensitivity to circumvent our previous failure to measure alpha chain binding to IL-2 variants (unpublished results).…”

Section: Discussionmentioning

confidence: 99%

Molecular dissection of the interactions of an antitumor interleukin‐2‐derived mutein on a phage display‐based platform

Rojas

Carmenate

León

2015

J of Molecular Recognition

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A mutein with stronger antitumor activity and lower toxicity than wild-type human interleukin-2 (IL-2) has been recently described. The rationale behind its design was to reinforce the immunostimulatory potential through the introduction of four mutations that would selectively disrupt the interaction with the IL-2 receptor alpha chain (thought to be critical for both IL-2-driven expansion of T regulatory cells and IL-2-mediated toxic effects). Despite the successful results of the mutein in several tumor models, characterization of its interactions was still to be performed. The current work, based on phage display of IL-2-derived variants, showed the individual contribution of each mutation to the impairment of alpha chain binding. A more sensitive assay, based on the ability of phage-displayed IL-2 variants to induce proliferation of the IL-2-dependent CTLL-2 cell line, revealed differences between the mutated variants. The results validated the mutein design, highlighting the importance of the combined effects of the four mutations. The developed phage display-based platform is robust and sensitive, allows a fast comparative evaluation of multiple variants, and could be broadly used to engineer IL-2 and related cytokines, accelerating the development of cytokine-derived therapeutics.

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“…This adaptable tool fuels innovative strategies for disease detection and the exploration of biomarkers. Once engineered, these modified phages are useful in diverse diagnostic applications, offering sensitive and precise detection of disease-related entities in clinical samples, ranging from minute targets such as viruses to substantial entities such as cancer cells ( Table 1 ) [ 182 , 183 , 184 , 185 , 186 ].…”

Section: Biotechnological Approaches To Bacteriophagementioning

confidence: 99%

The Biotechnological Application of Bacteriophages: What to Do and Where to Go in the Middle of the Post-Antibiotic Era

Jo,

Kwon,

Kim

et al. 2023

Microorganisms

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Amid the escalating challenges of antibiotic resistance, bacterial infections have emerged as a global threat. Bacteriophages (phages), viral entities capable of selectively infecting bacteria, are gaining momentum as promising alternatives to traditional antibiotics. Their distinctive attributes, including host specificity, inherent self-amplification, and potential synergy with antibiotics, render them compelling candidates. Phage engineering, a burgeoning discipline, involves the strategic modification of bacteriophages to enhance their therapeutic potential and broaden their applications. The integration of CRISPR-Cas systems facilitates precise genetic modifications, enabling phages to serve as carriers of functional genes/proteins, thereby enhancing diagnostics, drug delivery, and therapy. Phage engineering holds promise in transforming precision medicine, addressing antibiotic resistance, and advancing diverse applications. Emphasizing the profound therapeutic potential of phages, this review underscores their pivotal role in combatting bacterial diseases and highlights their significance in the post-antibiotic era.

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Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals

Cited by 8 publications

References 32 publications

Beyond natural antibodies: the power of in vitro display technologies

Beyond natural antibodies: the power of in vitro display technologies

Molecular dissection of the interactions of an antitumor interleukin‐2‐derived mutein on a phage display‐based platform

The Biotechnological Application of Bacteriophages: What to Do and Where to Go in the Middle of the Post-Antibiotic Era

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