Phage   Interaction with Vibrio Cholerae

Abstract

SUMMARY. The role of temperate phage p in determining the serology and eltor-lytic phage sensitivity in Vibrio cholerae was investigated. The only serological change found in six host strains was a change to roughness. This was accompanied by failure to adsorb several of the lytic phages. Various phage-sensitivity changes were induced by phage fl in two hosts at the post-adsorption level. In strain HP47, three types of progeny were obtained of which one was universally resistant to lytic phages. These untypable lysogens were culturally stable but gave rise to segregants of the rare phage-type 6 on single colony selection.

Phage-induced change of toxigenesis in Vibrio cholerae

“…cholerae strains used were classical biotype strain 569B (serotype Inaba); phage propagating eltor biotype strain MAK757 (serotype Ogawa) ; immunotyping lysogens MAK757(a), MAK757(VcA-1) and MAK757(B) (Siddiqui and Bhattacharyya, 1984) ; K-class phage indicator strains H218 and MAK757.…”

“…Bacteriophages used were lytic typing phages of the eltor and classical sets; temperate K-class phage a and VcA-2, phage p and heteroimmune phage VcA-1 (Siddiqui and Bhattacharyya, 1982and 1984.…”

“…1986; accepted 1 Jul. 1986. its lysogens-has been described-elsewhere (Siddiqui and Ghosh, 1983;Siddiqui and Bhattacharyya, 1984). The cultures and their derivatives were monitored for serotype and biotype stability and their phages characterised by immunotyping.…”

“…The cultures and their derivatives were monitored for serotype and biotype stability and their phages characterised by immunotyping. This was done by spotting phage lysates of the cultures on the three immunotyping lysogens and also testing their sensitivity to the immunotype phages in a similar manner (Siddiqui and Bhattacharyya, 1984).…”

Mutation induced by vibriophage PS166 infection changes biotype and phage type of Vibrio cholerae

“…In recent years, phage-induced changes in V. cholerae have been characterised at the serological level (Ogg et al, 1978;Shigeno, 1982;Siddiqui and Bhattacharyya, 1984). This paper describes mutants of V. cholerae strain MAK757 resistant to a newlyisolated vibrio phage PS166 and their serological characterisation.…”