Pharmaceutical and biomedical applications of affinity chromatography: Recent trends and developments

Abstract

Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically-related agent as a stationary phase to selectively retain analytes or to study biological interactions. This review discusses the basic principles behind affinity chromatography and examines recent developments that have occurred in the use of this method for biomedical and pharmaceutical analysis. Techniques based on traditional affinity supports are discussed, but an emphasis is placed on methods in which affinity columns are used as part of HPLC systems or in combination with other analytical methods. General formats for affinity chromatography that are considered include step elution schemes, weak affinity chromatography, affinity extraction and affinity depletion. Specific separation techniques that are examined include lectin affinity chromatography, boronate affinity chromatography, immunoaffinity chromatography, and immobilized metal ion affinity chromatography. Approaches for the study of biological interactions by affinity chromatography are also presented, such as the measurement of equilibrium constants, rate constants, or competition and displacement effects. In addition, related developments in the use of immobilized enzyme reactors, molecularly imprinted polymers, dye ligands and aptamers are briefly considered.

Bromelain: an overview of industrial application and purification strategies

“…Compared with papain and ficin, bromelain is commercially available in the market under name brands such as McCormick and Knorr. Commercial papain, bromelain, and zingibain efficiently hydrolyze a few meat myofibril proteins, such as actomysin, titin, and nebulin, as revealed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) (Hage et al 2012). Sullivan and Calkins (2010) reported that bromelain can improve the sensory evaluation rating and tenderness of meat comparable to other exogenous enzymes.…”

Overview of Albumin and Its Purification Methods

“…The purpose of this method is to selectively purify analytics and inspect the biological interactions between molecules. [82][83][84][85] Affinity chromatography was first introduced for the purpose of purification by Cuatecasas, et al in 1968. 86 There are different types of affinity ligands such as protein, enzyme, enzyme substrate or inhibitor, antibody, antigen, hormone, biomimetic dye and etc.…”

“…Depending on the purpose, selected type of ligand is immobilized within the column and further purification process is done following selective binding. 82 Here we describe some:…”

Proteomic Analysis of Stored Core Oil Palm Trunk (COPT) Sap Identifying Proteins Related to Stress, Disease Resistance and Differential Gene/Protein Expression

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